178

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/PHD-RelE
Location 425627..426174 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name relE Uniprot ID Q9KM92
Locus tag VC_RS15765 Protein ID WP_000229317.1
Coordinates 425872..426174 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KM93
Locus tag VC_RS15760 Protein ID WP_000861987.1
Coordinates 425627..425884 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15710 (VC_A0479) 420656..421111 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VC_RS15715 (VC_A0480) 421433..421585 + 153 WP_001884520.1 DUF645 family protein -
VC_RS15720 (VC_A0481) 421787..422284 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VC_RS15725 (VC_A0482) 422281..422553 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VC_RS15730 (VC_A0483) 422784..423452 + 669 WP_000043871.1 hypothetical protein -
VC_RS15735 (VC_A0484) 423604..423891 + 288 WP_000426470.1 hypothetical protein -
VC_RS15740 (VC_A0485) 424032..424403 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VC_RS15745 (VC_A0486) 424470..424895 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VC_RS15750 (VC_A0487) 424892..425425 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
VC_RS15760 (VC_A0488) 425627..425884 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
VC_RS15765 (VC_A0489) 425872..426174 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VC_RS15770 (VC_A0490) 426408..427325 + 918 WP_000186333.1 alpha/beta hydrolase -
VC_RS15775 (VC_A0491) 427482..427871 + 390 WP_001081302.1 hypothetical protein -
VC_RS15780 (VC_A0492) 428007..428216 + 210 Protein_466 GNAT family N-acetyltransferase -
VC_RS15785 (VC_A0493) 428335..428772 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VC_RS15790 (VC_A0494) 428836..429054 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VC_RS15795 (VC_A0495) 429229..430101 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VC_RS15800 (VC_A0496) 430251..430850 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VC_RS15805 430907..431011 + 105 WP_099607150.1 acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 428335..428772 437
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11726.68 Da        Isoelectric Point: 5.1972

>T178 WP_000229317.1 NC_002506:425872-426174 [Vibrio cholerae O1 biovar El Tor str. N16961]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Download         Length: 303 bp

>T178 NC_002506:425872-426174 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9647.15 Da        Isoelectric Point: 7.3178

>AT178 WP_000861987.1 NC_002506:425627-425884 [Vibrio cholerae O1 biovar El Tor str. N16961]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Download         Length: 258 bp

>AT178 NC_002506:425627-425884 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T172 Vibrio cholerae O1 biovar El Tor str. N16961

42.683

97.619

0.417
T1008 Escherichia coli O157:H7 str. Sakai

32.632

100

0.337
T6134 Paracoccus aminophilus

32.653

98.99

0.323
T10141 Pseudomonas savastanoi

32.292

98.969

0.32
T6097 Sinorhizobium meliloti 1021

34.831

91.753

0.32
T10119 Escherichia coli

33.721

93.478

0.315
T229 Caulobacter crescentus CB15

34.066

91

0.31
T1006 Escherichia coli O157:H7 str. Sakai

31.183

97.895

0.305

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10100 Pseudomonas aeruginosa PAO1

38.824

100

0.398

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM92

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SLM9

References

(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental


experimental experimental literature