Detailed information of TA system TA058323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 593931..594490
• Replicon: chromosome
• Accession: NZ_CP013310
• Organism: Vibrio cholerae strain E1162

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q9K2M8
• Locus tag: ASZ87_RS17815
• Protein ID: WP_000578476.1
• Coordinates: 593931..594209 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9L991
• Locus tag: ASZ87_RS17820
• Protein ID: WP_000381183.1
• Coordinates: 594206..594490 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ87_RS17765	589231..589668	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ87_RS17770	589732..589950	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ87_RS17780	590125..590997	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
ASZ87_RS17785	591147..591746	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
ASZ87_RS20835	591803..591907	+	105	WP_099607150.1	acetyltransferase	-
ASZ87_RS17790	591938..592207	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
ASZ87_RS17795	592201..592722	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
ASZ87_RS20840	593021..593146	+	126	WP_001905700.1	DUF645 family protein	-
ASZ87_RS17810	593397..593792	+	396	WP_001000867.1	hypothetical protein	-
ASZ87_RS17815	593931..594209	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
ASZ87_RS17820	594206..594490	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
ASZ87_RS17825	594684..595199	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
ASZ87_RS17830	595383..595796	+	414	WP_000049420.1	VOC family protein	-
ASZ87_RS17845	596351..597528	+	1178	WP_086010879.1	IS3-like element ISVch4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	435534..596659	161125
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.005	inside	Integron	-	-	440614..596283	155669
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	589231..589668	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10819.59 Da
• Isoelectric Point: 5.1651

>T58323 WP_000578476.1 NZ_CP013310:c594209-593931 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Nucleotide

• Length: 279 bp

>T58323 NZ_CP013310:c594209-593931 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10901.25 Da
• Isoelectric Point: 8.0775

>AT58323 WP_000381183.1 NZ_CP013310:c594490-594206 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Nucleotide

• Length: 285 bp

>AT58323 NZ_CP013310:c594490-594206 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Structures

Toxin

• AlphaFold DB: Q9K2M8

Antitoxin

• AlphaFold DB: A0A067BL33