176

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) /RHH-GNAT
Location 421787..422553 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag VC_RS15720 Protein ID WP_000982260.1
Coordinates 421787..422284 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag VC_RS15725 Protein ID WP_000246253.1
Coordinates 422281..422553 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS15675 (VC_A0473) 417649..417955 + 307 Protein_446 CatB-related O-acetyltransferase -
VC_RS15680 (VC_A0474) 418092..418784 + 693 WP_001047169.1 GNAT family N-acetyltransferase -
VC_RS15685 (VC_A0475) 418784..419185 + 402 WP_000351248.1 type II toxin-antitoxin system death-on-curing family toxin -
VC_RS15690 419155..419298 + 144 WP_071908341.1 DUF3265 domain-containing protein -
VC_RS15695 (VC_A0476) 419373..419786 + 414 WP_000049417.1 VOC family protein -
VC_RS15700 (VC_A0477) 420000..420251 + 252 WP_001250179.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15705 (VC_A0478) 420241..420528 + 288 WP_000221354.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15710 (VC_A0479) 420656..421111 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VC_RS15715 (VC_A0480) 421433..421585 + 153 WP_001884520.1 DUF645 family protein -
VC_RS15720 (VC_A0481) 421787..422284 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
VC_RS15725 (VC_A0482) 422281..422553 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
VC_RS15730 (VC_A0483) 422784..423452 + 669 WP_000043871.1 hypothetical protein -
VC_RS15735 (VC_A0484) 423604..423891 + 288 WP_000426470.1 hypothetical protein -
VC_RS15740 (VC_A0485) 424032..424403 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VC_RS15745 (VC_A0486) 424470..424895 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VC_RS15750 (VC_A0487) 424892..425425 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
VC_RS15760 (VC_A0488) 425627..425884 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VC_RS15765 (VC_A0489) 425872..426174 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
VC_RS15770 (VC_A0490) 426408..427325 + 918 WP_000186333.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 416601..417581 980
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T176 WP_000982260.1 NC_002506:c422284-421787 [Vibrio cholerae O1 biovar El Tor str. N16961]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T176 NC_002506:c422284-421787 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT176 WP_000246253.1 NC_002506:c422553-422281 [Vibrio cholerae O1 biovar El Tor str. N16961]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT176 NC_002506:c422553-422281 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T165 Vibrio cholerae O1 biovar El Tor str. N16961

100

100

1
T10120 Klebsiella pneumoniae subsp. pneumoniae HS11286

32.716

98.182

0.321

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT165 Vibrio cholerae O1 biovar El Tor str. N16961

100

100

1
AT10120 Klebsiella pneumoniae subsp. pneumoniae HS11286

32.927

93.182

0.307
AT200 Escherichia coli O157:H7 str. EDL933

33.333

92.045

0.307

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References

(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental


experimental experimental literature