Detailed information of TA system TA145810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 369827..370442
• Replicon: chromosome
• Accession: NZ_CP047296
• Organism: Vibrio cholerae C6706

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: GTF72_RS15590
• Protein ID: WP_001162670.1
• Coordinates: 369827..370114 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTF72_RS15595
• Protein ID: WP_001232701.1
• Coordinates: 370125..370442 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTF72_RS15550	365463..365558	+	96	WP_001907607.1	DUF645 family protein	-
GTF72_RS15555	365752..366069	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GTF72_RS15560	366088..366330	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GTF72_RS15565	366572..367081	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
GTF72_RS15570	367138..367791	+	654	WP_000226874.1	hypothetical protein	-
GTF72_RS15575	368101..368319	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
GTF72_RS15580	368722..368955	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
GTF72_RS15585	369380..369560	+	181	Protein_365	DUF645 family protein	-
GTF72_RS15590	369827..370114	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTF72_RS15595	370125..370442	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
GTF72_RS15600	370439..370549	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
GTF72_RS15605	370726..370851	+	126	WP_001944767.1	DUF645 family protein	-
GTF72_RS15610	370867..370905	+	39	WP_106019119.1	hypothetical protein	-
GTF72_RS15615	371129..371869	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
GTF72_RS15620	372074..372904	+	831	WP_000056616.1	abortive infection family protein	-
GTF72_RS15625	372961..373443	+	483	WP_001895003.1	hypothetical protein	-
GTF72_RS15630	373890..374765	+	876	WP_000259044.1	hypothetical protein	-
GTF72_RS15635	374895..375350	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	306905..436165	129260
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0	inside	Integron	-	-	311985..435789	123804

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T145810 WP_001162670.1 NZ_CP047296:369827-370114 [Vibrio cholerae C6706]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T145810 NZ_CP062700:1080203-1080538 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT145810 WP_001232701.1 NZ_CP047296:370125-370442 [Vibrio cholerae C6706]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT145810 NZ_CP062700:1079955-1080203 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36