Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | parDE/PHD-RelE |
Location | 322686..323264 | Replicon | chromosome II |
Accession | NC_002506 | ||
Organism | Vibrio cholerae O1 biovar El Tor str. N16961 |
Toxin (Protein)
Gene name | parE | Uniprot ID | O68848 |
Locus tag | VC_RS14830 | Protein ID | WP_001180243.1 |
Coordinates | 322686..323003 (-) | Length | 106 a.a. |
Antitoxin (Protein)
Gene name | parD | Uniprot ID | Q7DCR7 |
Locus tag | VC_RS14835 | Protein ID | WP_000557292.1 |
Coordinates | 323022..323264 (-) | Length | 81 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
VC_RS14800 (VC_A0304) | 318210..318392 | + | 183 | WP_000923340.1 | DUF645 family protein | - |
VC_RS14805 (VC_A0306) | 318746..318928 | + | 183 | WP_000923153.1 | DUF645 family protein | - |
VC_RS14810 (VC_A0307) | 319123..319800 | + | 678 | WP_000254617.1 | HNH endonuclease | - |
VC_RS14815 (VC_A0308) | 319962..321170 | + | 1209 | WP_000272282.1 | deoxyguanosinetriphosphate triphosphohydrolase family protein | - |
VC_RS14820 (VC_A0309) | 321332..322036 | + | 705 | WP_000087610.1 | HNH endonuclease | - |
VC_RS14825 (VC_A0310) | 322194..322532 | + | 339 | WP_000713853.1 | hypothetical protein | - |
VC_RS14830 (VC_A0311) | 322686..323003 | - | 318 | WP_001180243.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
VC_RS14835 (VC_A0312) | 323022..323264 | - | 243 | WP_000557292.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
VC_RS14840 (VC_A0313) | 323508..323894 | + | 387 | WP_001015867.1 | NUDIX hydrolase | - |
VC_RS14845 | 323951..324064 | + | 114 | WP_001900214.1 | hypothetical protein | - |
VC_RS14850 (VC_A0314) | 324013..324294 | + | 282 | WP_001894429.1 | DUF1289 domain-containing protein | - |
VC_RS14860 (VC_A0316) | 324552..325088 | + | 537 | WP_000469482.1 | GNAT family N-acetyltransferase | - |
VC_RS14865 (VC_A0317) | 325225..325740 | + | 516 | WP_001201509.1 | lipocalin family protein | - |
VC_RS14870 (VC_A0318) | 325890..326387 | - | 498 | WP_000982260.1 | GNAT family N-acetyltransferase | - |
VC_RS14875 (VC_A0319) | 326384..326656 | - | 273 | WP_000246253.1 | DUF1778 domain-containing protein | - |
VC_RS14880 | 327049..327174 | + | 126 | WP_001900195.1 | DUF645 family protein | - |
VC_RS14885 | 327190..327228 | + | 39 | WP_106019118.1 | hypothetical protein | - |
VC_RS14890 (VC_A0322) | 327424..327870 | + | 447 | WP_000006157.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
inside | Integron | catB9 | - | 309750..435387 | 125637 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 106 a.a. Molecular weight: 12158.97 Da Isoelectric Point: 9.7495
>T164 WP_001180243.1 NC_002506:c323003-322686 [Vibrio cholerae O1 biovar El Tor str. N16961]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS
Download Length: 318 bp
>T164 NC_002506:c323003-322686 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG
Antitoxin
Download Length: 81 a.a. Molecular weight: 8961.25 Da Isoelectric Point: 5.6630
>AT164 WP_000557292.1 NC_002506:c323264-323022 [Vibrio cholerae O1 biovar El Tor str. N16961]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL
Download Length: 243 bp
>AT164 NC_002506:c323264-323022 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
T170 | Vibrio cholerae O1 biovar El Tor str. N16961 |
100 |
100 |
1 |
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT170 | Vibrio cholerae O1 biovar El Tor str. N16961 |
100 |
100 |
1 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0K9UJR8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | Q7DCR7 |
References
(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030]
(2) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445]
experimental literature