Detailed information of TA system TA058258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 325571..326337
• Replicon: chromosome
• Accession: NZ_CP013306
• Organism: Vibrio cholerae strain CRC1106

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A233HKB4
• Locus tag: ASZ81_RS15730
• Protein ID: WP_000982256.1
• Coordinates: 325571..326068 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ81_RS15735
• Protein ID: WP_000246254.1
• Coordinates: 326065..326337 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ81_RS15695	320971..321510	+	540	WP_053035032.1	phosphoglycerate mutase family protein	-
ASZ81_RS15700	321645..322070	+	426	WP_000693748.1	hypothetical protein	-
ASZ81_RS20905	322127..322237	+	111	WP_106746893.1	iron transporter	-
ASZ81_RS15705	322224..322583	+	360	WP_001071527.1	VOC family protein	-
ASZ81_RS21005	322725..323158	+	434	Protein_277	GNAT family N-acetyltransferase	-
ASZ81_RS15715	323295..324221	+	927	WP_000377813.1	hypothetical protein	-
ASZ81_RS20370	324544..324697	+	154	Protein_279	DUF645 family protein	-
ASZ81_RS15725	324926..325429	+	504	WP_001158950.1	DinB family protein	-
ASZ81_RS15730	325571..326068	-	498	WP_000982256.1	GNAT family N-acetyltransferase	Toxin
ASZ81_RS15735	326065..326337	-	273	WP_000246254.1	DUF1778 domain-containing protein	Antitoxin
ASZ81_RS15740	326725..326892	+	168	WP_053035038.1	DUF645 family protein	-
ASZ81_RS15745	327100..327447	+	348	WP_000933413.1	hypothetical protein	-
ASZ81_RS15755	328132..328314	+	183	WP_000923340.1	DUF645 family protein	-
ASZ81_RS15760	328529..329068	+	540	WP_000099372.1	hypothetical protein	-
ASZ81_RS15765	329191..329706	+	516	WP_000090011.1	hypothetical protein	-
ASZ81_RS15770	329841..330377	+	537	WP_000644491.1	nucleotidyltransferase family protein	-
ASZ81_RS15775	330846..330956	+	111	WP_071908335.1	DUF3265 domain-containing protein	-
ASZ81_RS15785	331169..331321	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304741..463550	158809
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.006	inside	Integron	-	-	309821..463174	153353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18539.23 Da
• Isoelectric Point: 8.7753

>T58258 WP_000982256.1 NZ_CP013306:c326068-325571 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Nucleotide

• Length: 498 bp

>T58258 NZ_CP013306:c326068-325571 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCATTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACACCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCTTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCGCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCTGTTGTGATCGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCTGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9685.19 Da
• Isoelectric Point: 6.2988

>AT58258 WP_000246254.1 NZ_CP013306:c326337-326065 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVANAKLKL
ASERYESKTQ

Nucleotide

• Length: 273 bp

>AT58258 NZ_CP013306:c326337-326065 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCGGCAATTGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGCAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Structures

Toxin

• AlphaFold DB: A0A233HKB4