168

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) relBE/RHH-RelE
Location 343243..343886 Replicon chromosome II
Accession NC_002506
Organism Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag VC_RS15045 Protein ID WP_000578476.1
Coordinates 343243..343521 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag VC_RS15050 Protein ID WP_000381183.1
Coordinates 343518..343802 (-) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC_RS14995 (VC_A0340) 338321..338827 + 507 WP_000393074.1 hypothetical protein -
VC_RS15000 (VC_A0341) 338984..339370 + 387 WP_000703163.1 VOC family protein -
VC_RS15005 (VC_A0342) 339627..339830 + 204 WP_001911580.1 hypothetical protein -
VC_RS15010 (VC_A0343) 340025..340276 + 252 WP_001890111.1 TIGR03643 family protein -
VC_RS15015 340249..340398 + 150 WP_071908333.1 DUF3265 domain-containing protein -
VC_RS15020 (VC_A0344) 340545..340970 + 426 WP_000415750.1 hypothetical protein -
VC_RS15025 (VC_A0345) 341176..341799 + 624 WP_000247070.1 DUF1349 domain-containing protein -
VC_RS15030 (VC_A0346) 342012..342491 + 480 WP_000009888.1 lecithin retinol acyltransferase family protein -
VC_RS15035 (VC_A0347) 342679..343092 + 414 WP_000049420.1 VOC family protein -
VC_RS15045 (VC_A0348) 343243..343521 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VC_RS15050 (VC_A0349) 343518..343802 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VC_RS15055 (VC_A0350) 343997..344512 + 516 WP_001201520.1 lipocalin family protein -
VC_RS15060 (VC_A0351) 344577..345173 + 597 WP_000255434.1 hypothetical protein -
VC_RS15065 (VC_A0353) 345564..345746 + 183 WP_000923182.1 DUF645 family protein -
VC_RS15070 (VC_A0354) 345946..346419 + 474 WP_001161076.1 GrpB family protein -
VC_RS15075 346434..346526 + 93 WP_014378730.1 DUF3265 domain-containing protein -
VC_RS15080 (VC_A0355) 346568..347194 + 627 WP_000365424.1 LysE family translocator -
VC_RS15085 (VC_A0356) 347317..348015 + 699 WP_001890502.1 hypothetical protein -
VC_RS15090 348025..348135 + 111 WP_079857360.1 DUF3265 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integron catB9 - 309750..435387 125637


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T168 WP_000578476.1 NC_002506:c343521-343243 [Vibrio cholerae O1 biovar El Tor str. N16961]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T168 NC_002506:c343521-343243 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT168 WP_000381183.1 NC_002506:c343802-343518 [Vibrio cholerae O1 biovar El Tor str. N16961]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT168 NC_002506:c343802-343518 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T179 Vibrio cholerae O1 biovar El Tor str. N16961

100

100

1

T705 Vibrio parahaemolyticus RIMD 2210633

84.783

100

0.848

T6097 Sinorhizobium meliloti 1021

38.462

98.913

0.38

T10119 Escherichia coli

34.783

100

0.348


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT179 Vibrio cholerae O1 biovar El Tor str. N16961

100

100

1

AT705 Vibrio parahaemolyticus RIMD 2210633

90.217

97.872

0.883


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
AlphaFold DB Q9K2M8


Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References


(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030] experimental

(2) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445] experimental


experimental experimental literature