Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | relBE/RHH-RelE |
Location | 343243..343886 | Replicon | chromosome II |
Accession | NC_002506 | ||
Organism | Vibrio cholerae O1 biovar El Tor str. N16961 |
Toxin (Protein)
Gene name | relE | Uniprot ID | Q9K2M8 |
Locus tag | VC_RS15045 | Protein ID | WP_000578476.1 |
Coordinates | 343243..343521 (-) | Length | 93 a.a. |
Antitoxin (Protein)
Gene name | relB | Uniprot ID | Q9L991 |
Locus tag | VC_RS15050 | Protein ID | WP_000381183.1 |
Coordinates | 343518..343802 (-) | Length | 95 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
VC_RS14995 (VC_A0340) | 338321..338827 | + | 507 | WP_000393074.1 | hypothetical protein | - |
VC_RS15000 (VC_A0341) | 338984..339370 | + | 387 | WP_000703163.1 | VOC family protein | - |
VC_RS15005 (VC_A0342) | 339627..339830 | + | 204 | WP_001911580.1 | hypothetical protein | - |
VC_RS15010 (VC_A0343) | 340025..340276 | + | 252 | WP_001890111.1 | TIGR03643 family protein | - |
VC_RS15015 | 340249..340398 | + | 150 | WP_071908333.1 | DUF3265 domain-containing protein | - |
VC_RS15020 (VC_A0344) | 340545..340970 | + | 426 | WP_000415750.1 | hypothetical protein | - |
VC_RS15025 (VC_A0345) | 341176..341799 | + | 624 | WP_000247070.1 | DUF1349 domain-containing protein | - |
VC_RS15030 (VC_A0346) | 342012..342491 | + | 480 | WP_000009888.1 | lecithin retinol acyltransferase family protein | - |
VC_RS15035 (VC_A0347) | 342679..343092 | + | 414 | WP_000049420.1 | VOC family protein | - |
VC_RS15045 (VC_A0348) | 343243..343521 | - | 279 | WP_000578476.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | Toxin |
VC_RS15050 (VC_A0349) | 343518..343802 | - | 285 | WP_000381183.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
VC_RS15055 (VC_A0350) | 343997..344512 | + | 516 | WP_001201520.1 | lipocalin family protein | - |
VC_RS15060 (VC_A0351) | 344577..345173 | + | 597 | WP_000255434.1 | hypothetical protein | - |
VC_RS15065 (VC_A0353) | 345564..345746 | + | 183 | WP_000923182.1 | DUF645 family protein | - |
VC_RS15070 (VC_A0354) | 345946..346419 | + | 474 | WP_001161076.1 | GrpB family protein | - |
VC_RS15075 | 346434..346526 | + | 93 | WP_014378730.1 | DUF3265 domain-containing protein | - |
VC_RS15080 (VC_A0355) | 346568..347194 | + | 627 | WP_000365424.1 | LysE family translocator | - |
VC_RS15085 (VC_A0356) | 347317..348015 | + | 699 | WP_001890502.1 | hypothetical protein | - |
VC_RS15090 | 348025..348135 | + | 111 | WP_079857360.1 | DUF3265 domain-containing protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
inside | Integron | catB9 | - | 309750..435387 | 125637 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10819.59 Da Isoelectric Point: 5.1651
>T168 WP_000578476.1 NC_002506:c343521-343243 [Vibrio cholerae O1 biovar El Tor str. N16961]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND
Download Length: 279 bp
>T168 NC_002506:c343521-343243 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10901.25 Da Isoelectric Point: 8.0775
>AT168 WP_000381183.1 NC_002506:c343802-343518 [Vibrio cholerae O1 biovar El Tor str. N16961]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA
Download Length: 285 bp
>AT168 NC_002506:c343802-343518 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
T179 | Vibrio cholerae O1 biovar El Tor str. N16961 |
100 |
100 |
1 |
T705 | Vibrio parahaemolyticus RIMD 2210633 |
84.783 |
100 |
0.848 |
T6097 | Sinorhizobium meliloti 1021 |
38.462 |
98.913 |
0.38 |
T10119 | Escherichia coli |
34.783 |
100 |
0.348 |
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT179 | Vibrio cholerae O1 biovar El Tor str. N16961 |
100 |
100 |
1 |
AT705 | Vibrio parahaemolyticus RIMD 2210633 |
90.217 |
97.872 |
0.883 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | Q9K2M8 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A067BL33 |
References
(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030]
(2) Evelyne Krin et al. (2023) Systematic transcriptome analysis allows the identification of new type I and type II Toxin/Antitoxin systems located in the superintegron of Vibrio cholerae. Research in Microbiology 174(1-2):103997. [PubMed:36347445]
experimental literature