Detailed information of TA system TA230644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 876838..877453
• Replicon: chromosome
• Accession: NZ_CP090400
• Organism: Vibrio cholerae strain PanChol

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: LZM82_RS17625
• Protein ID: WP_001162670.1
• Coordinates: 877166..877453 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZM82_RS17620
• Protein ID: WP_001232701.1
• Coordinates: 876838..877155 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZM82_RS17570 (LZM82_17570)	872247..872708	-	462	WP_001911578.1	lipocalin family protein	-
LZM82_RS17575 (LZM82_17575)	872899..873375	-	477	WP_001047180.1	GNAT family N-acetyltransferase	-
LZM82_RS17580 (LZM82_17580)	873403..873513	-	111	WP_071917832.1	DUF3265 domain-containing protein	-
LZM82_RS17585 (LZM82_17585)	873510..874439	-	930	WP_000335802.1	hypothetical protein	-
LZM82_RS17590 (LZM82_17590)	874559..875005	-	447	WP_000128977.1	hypothetical protein	-
LZM82_RS17595 (LZM82_17595)	875158..876162	-	1005	WP_000964925.1	LD-carboxypeptidase	-
LZM82_RS17600 (LZM82_17600)	876350..876466	-	117	WP_001889170.1	hypothetical protein	-
LZM82_RS17605 (LZM82_17605)	876429..876554	-	126	WP_001944767.1	DUF645 family protein	-
LZM82_RS17610 (LZM82_17610)	876582..876692	-	111	Protein_790	acetyltransferase	-
LZM82_RS17615 (LZM82_17615)	876731..876841	-	111	WP_134814058.1	DUF3265 domain-containing protein	-
LZM82_RS17620 (LZM82_17620)	876838..877155	-	318	WP_001232701.1	HigA family addiction module antitoxin	Antitoxin
LZM82_RS17625 (LZM82_17625)	877166..877453	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZM82_RS17630 (LZM82_17630)	877494..877607	-	114	WP_001894955.1	hypothetical protein	-
LZM82_RS17635 (LZM82_17635)	877720..877900	-	181	Protein_795	DUF645 family protein	-
LZM82_RS17640 (LZM82_17640)	878325..878558	-	234	WP_032482776.1	DUF3709 domain-containing protein	-
LZM82_RS17645 (LZM82_17645)	878579..878772	-	194	Protein_797	hypothetical protein	-
LZM82_RS17650 (LZM82_17650)	878961..879179	-	219	WP_001917086.1	DUF3709 domain-containing protein	-
LZM82_RS17655 (LZM82_17655)	879191..879400	-	210	WP_001900246.1	DUF3709 domain-containing protein	-
LZM82_RS17660 (LZM82_17660)	879489..880142	-	654	WP_000226874.1	hypothetical protein	-
LZM82_RS17665 (LZM82_17665)	880199..880708	-	510	WP_000405987.1	GNAT family N-acetyltransferase	-
LZM82_RS17670 (LZM82_17670)	880950..881192	+	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LZM82_RS17675 (LZM82_17675)	881211..881528	+	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZM82_RS17680 (LZM82_17680)	881722..881874	-	153	Protein_804	DUF645 family protein	-
LZM82_RS17685 (LZM82_17685)	881935..882110	-	176	Protein_805	acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	835643..943910	108267
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA000164, TA000169, TA000166, TA000167, TA000179, TA000170, TA000171, TA000172, TA000173, TA000174, TA000175, TA000176, TA000177, TA000178, TA000168, TA000165, TA010030, TA010035 309750..435387 0.004	inside	Integron	-	-	836019..937068	101049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T230644 WP_001162670.1 NZ_CP090400:c877453-877166 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T230644 NZ_CP125223:c2389019-2388837 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATAGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACGT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT230644 WP_001232701.1 NZ_CP090400:c877155-876838 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT230644 NZ_CP125223:c2388744-2388340 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: A0A0F2HI36