Detailed information of TA system TA000166

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH-RelE
• Location: 328018..328558
• Replicon: chromosome II
• Accession: NC_002506
• Organism: Vibrio cholerae O1 biovar El Tor str. N16961

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A151JGK8
• Locus tag: VC_RS14895
• Protein ID: WP_000277238.1
• Coordinates: 328018..328287 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KML3
• Locus tag: VC_RS14900
• Protein ID: WP_001258569.1
• Coordinates: 328280..328558 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC_RS14835 (VC_A0312)	323022..323264	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC_RS14840 (VC_A0313)	323508..323894	+	387	WP_001015867.1	NUDIX hydrolase	-
VC_RS14845	323951..324064	+	114	WP_001900214.1	hypothetical protein	-
VC_RS14850 (VC_A0314)	324013..324294	+	282	WP_001894429.1	DUF1289 domain-containing protein	-
VC_RS14860 (VC_A0316)	324552..325088	+	537	WP_000469482.1	GNAT family N-acetyltransferase	-
VC_RS14865 (VC_A0317)	325225..325740	+	516	WP_001201509.1	lipocalin family protein	-
VC_RS14870 (VC_A0318)	325890..326387	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VC_RS14875 (VC_A0319)	326384..326656	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
VC_RS14880	327049..327174	+	126	WP_001900195.1	DUF645 family protein	-
VC_RS14885	327190..327228	+	39	WP_106019118.1	hypothetical protein	-
VC_RS14890 (VC_A0322)	327424..327870	+	447	WP_000006157.1	hypothetical protein	-
VC_RS14895 (VC_A0323)	328018..328287	-	270	WP_000277238.1	type II toxin-antitoxin system YafQ family toxin	Toxin
VC_RS14900 (VC_A0324)	328280..328558	-	279	WP_001258569.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
VC_RS14905 (VC_A0325)	328844..329122	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
VC_RS14910 (VC_A0327)	329374..329580	+	207	Protein_292	DUF3709 domain-containing protein	-
VC_RS14915	329780..329881	+	102	WP_001921603.1	hypothetical protein	-
VC_RS14920 (VC_A0328)	329871..330257	+	387	WP_000703163.1	VOC family protein	-
VC_RS14925 (VC_A0329)	330452..330703	+	252	WP_001890111.1	TIGR03643 family protein	-
VC_RS14930	330676..330825	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
VC_RS14935 (VCA0329a)	330917..331159	+	243	WP_000107461.1	hypothetical protein	-
VC_RS14940 (VCA0329b)	331411..331689	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
VC_RS18625	332064..332207	+	144	Protein_299	DUF645 family protein	-
VC_RS18630	332261..332299	+	39	WP_082798268.1	hypothetical protein	-
VC_RS14950 (VC_A0330)	332511..332978	+	468	WP_001289288.1	OsmC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_012667 Vibrio cholerae MJ-1236 Integron TA023781, TA023782, TA023783, TA023784, TA023785, TA023786, TA023787, TA023788, TA023789, TA023790, TA023791, TA023792, TA023793, TA023794, TA023795 958376..1050596 0.006 View NC_016446 Vibrio cholerae O1 str. 2010EL-1786 Integron TA025705, TA025706, TA025707, TA025708, TA025709, TA025710, TA025711, TA025712, TA025713, TA025714, TA025715, TA025716, TA025717, TA025718, TA025719, TA025720, TA025721, TA025722 36195..135928 0.004 View NC_016945 Vibrio cholerae IEC224 Integron TA026143, TA026144, TA026145, TA026146, TA026147, TA026148, TA026149, TA026150, TA026151, TA026152, TA026153, TA026154, TA026155, TA026156, TA026157, TA026158, TA026159, TA026160 309717..435206 0 View NZ_AP024548 Vibrio cholerae strain BCH-01536 Integron TA038617, TA038618, TA038619, TA038620, TA038621, TA038622, TA038623, TA038624, TA038625, TA038626, TA038627, TA038628, TA038629, TA038630, TA038631, TA038632 11978..113648 0.004 View NZ_AP024554 Vibrio cholerae strain IDH-03329 Integron TA038668, TA038669, TA038670, TA038671, TA038672, TA038673, TA038674, TA038675, TA038676, TA038677, TA038678, TA038679, TA038680, TA038681, TA038682, TA038683, TA038684 213119..312856 0.004 View NZ_CP006948 Vibrio cholerae O1 str. KW3 Integron TA045802, TA045803, TA045804, TA045805, TA045806, TA045807, TA045808, TA045809, TA045810, TA045811, TA045812, TA045813, TA045814, TA045815, TA045816, TA045817, TA045818, TA045819 309725..410760 0.004 View NZ_CP007635 Vibrio cholerae strain 2012EL-2176 Integron TA046749, TA046750, TA046751, TA046752, TA046753, TA046754, TA046755, TA046756, TA046757, TA046758, TA046759, TA046760, TA046761, TA046762, TA046763, TA046764, TA046765, TA046766 36117..137166 0.004 View NZ_CP009041 Vibrio cholerae O1 biovar El Tor strain FJ147 Integron TA048145, TA048146, TA048147, TA048148, TA048149, TA048150, TA048151, TA048152, TA048153, TA048154, TA048155, TA048156, TA048157, TA048158, TA048159, TA048160, TA048161, TA048162 309780..409516 0.004 View NZ_CP012666 Vibrio cholerae strain ICDC-VC661 Integron TA056924, TA056909, TA056910, TA056911, TA056912, TA056913, TA056914, TA056915, TA056916, TA056917, TA056918, TA056919, TA056920, TA056921, TA056922, TA056923, TA056925 309725..399516 0.005 View NZ_CP013302 Vibrio cholerae strain C5 Integron TA058213, TA058214, TA058215, TA058216, TA058217, TA058218, TA058219, TA058220, TA058221, TA058222, TA058223, TA058224, TA058225, TA058226, TA058227, TA058228, TA058229, TA058230, TA058231, TA058232, TA058233, TA058234, TA058235 309774..468910 0.005 View NZ_CP013306 Vibrio cholerae strain CRC1106 Integron TA058258, TA058259, TA058260, TA058261, TA058262, TA058263, TA058264, TA058265, TA058266, TA058267, TA058268, TA058269, TA058270, TA058271, TA058272, TA058273, TA058274, TA058275, TA058276, TA058277, TA058278, TA058279, TA058280 309821..463174 0.006 View NZ_CP013310 Vibrio cholerae strain E1162 Integron TA058301, TA058302, TA058303, TA058304, TA058305, TA058306, TA058307, TA058308, TA058309, TA058310, TA058311, TA058312, TA058313, TA058314, TA058315, TA058316, TA058317, TA058318, TA058319, TA058320, TA058321, TA058322, TA058323 440614..596283 0.005 View NZ_CP013314 Vibrio cholerae strain E9120 Integron TA058348, TA058349, TA058350, TA058351, TA058352, TA058353, TA058354, TA058355, TA058356, TA058357, TA058358, TA058359, TA058360, TA058361, TA058362, TA058363, TA058364, TA058365, TA058366, TA058367, TA058368, TA058369, TA058370 309780..468088 0.005 View NZ_CP013316 Vibrio cholerae strain M2140 Integron TA058372, TA058373, TA058374, TA058375, TA058376, TA058377, TA058378, TA058379, TA058380, TA058381, TA058382, TA058383, TA058384, TA058385, TA058386, TA058387, TA058388, TA058389 93061..221594 0.008 View NZ_CP013318 Vibrio cholerae strain NCTC5395 Integron TA058391, TA058392, TA058393, TA058394, TA058395, TA058396, TA058397, TA058398, TA058399, TA058400, TA058401, TA058402, TA058403, TA058404, TA058405, TA058406, TA058407, TA058408, TA058409, TA058410, TA058411 343365..496457 0.009 View NZ_CP020407 Vibrio cholerae strain FDAARGOS_223 Integron TA074614, TA074615, TA074616, TA074617, TA074618, TA074619, TA074620, TA074621, TA074622, TA074623, TA074624, TA074625, TA074626, TA074627, TA074628, TA074629, TA074630, TA074631 749463..875103 0 View NZ_CP024163 Vibrio cholerae strain E7946 Integron TA087016, TA087017, TA087018, TA087019, TA087020, TA087021, TA087022, TA087023, TA087024, TA087025, TA087026, TA087027, TA087028, TA087029, TA087030, TA087031, TA087032, TA087033 309306..433568 0 View NZ_CP024868 Vibrio cholerae strain A1552 Integron TA088996, TA088997, TA088987, TA088988, TA088989, TA088990, TA088991, TA088992, TA088993, TA088994, TA088995, TA088998, TA088999, TA089000, TA089001, TA089002, TA089003, TA089004 309797..433602 0 View NZ_CP025937 Vibrio cholerae strain A1552 Integron TA092502, TA092503, TA092504, TA092505, TA092506, TA092507, TA092508, TA092509, TA092510, TA092511, TA092512, TA092513, TA092514, TA092515, TA092516, TA092517, TA092518, TA092519 309147..432952 0 View NZ_CP026648 Vibrio cholerae O1 biovar El Tor strain HC1037 Integron TA095391, TA095375, TA095376, TA095377, TA095378, TA095379, TA095380, TA095381, TA095382, TA095383, TA095384, TA095385, TA095386, TA095387, TA095388, TA095389, TA095390, TA095392 36123..135858 0.004 View NZ_CP028828 Vibrio cholerae strain N16961 Integron TA104260, TA104261, TA104247, TA104248, TA104249, TA104250, TA104251, TA104252, TA104253, TA104254, TA104255, TA104256, TA104257, TA104258, TA104259, TA104262, TA104263, TA104264 312057..437697 0 View NZ_CP028895 Vibrio cholerae strain A1552 Integron TA104335, TA104336, TA104337, TA104338, TA104339, TA104340, TA104341, TA104342, TA104343, TA104344, TA104345, TA104346, TA104347, TA104348, TA104349, TA104350, TA104351, TA104352 311994..435799 0 View NZ_CP033513 Vibrio cholerae strain E4 Integron TA113805, TA113806, TA113807, TA113808, TA113794, TA113795, TA113796, TA113797, TA113798, TA113799, TA113800, TA113801, TA113802, TA113803, TA113804, TA113809, TA113810, TA113811 127139..226875 0.004 View NZ_CP040173 Vibrio cholerae strain VC1374 Integron TA125036, TA125037, TA125038, TA125039, TA125040, TA125041, TA125042, TA125043, TA125044, TA125045, TA125046, TA125047, TA125048, TA125049, TA125050, TA125051, TA125052 887669..977464 0.005 View NZ_CP046841 Vibrio cholerae strain F9993 Integron TA145056, TA145057, TA145058, TA145059, TA145060, TA145061, TA145062, TA145063, TA145064, TA145065, TA145066, TA145067, TA145068, TA145069, TA145070, TA145071, TA145072 322432..412236 0.005 View NZ_CP047060 Vibrio cholerae isolate CTMA_1441 Integron TA145398, TA145399, TA145392, TA145393, TA145394, TA145395, TA145396, TA145397, TA145400, TA145401, TA145402, TA145403, TA145404, TA145405, TA145406, TA145407, TA145408, TA145409 311040..410783 0.004 View NZ_CP047296 Vibrio cholerae C6706 Integron TA145820, TA145815, TA145816, TA145817, TA145818, TA145819, TA145803, TA145804, TA145805, TA145806, TA145807, TA145808, TA145809, TA145810, TA145811, TA145812, TA145813, TA145814, TA145821 311985..435789 0 View NZ_CP047298 Vibrio cholerae O1 biovar El Tor strain C6709 Integron TA145823, TA145824, TA145825, TA145826, TA145827, TA145828, TA145829, TA145830, TA145831, TA145832, TA145833, TA145834, TA145835, TA145836, TA145837, TA145838, TA145839, TA145840, TA145841 311981..435786 0 View NZ_CP047300 Vibrio cholerae O1 biovar El Tor strain P27459 Integron TA145843, TA145844, TA145845, TA145846, TA145847, TA145848, TA145849, TA145850, TA145851, TA145852, TA145853, TA145854, TA145855, TA145856, TA145857, TA145858, TA145859, TA145860, TA145861 312006..438916 0 View NZ_CP047302 Vibrio cholerae O1 biovar El Tor strain DRC-193A Integron TA145864, TA145865, TA145866, TA145867, TA145868, TA145869, TA145870, TA145871, TA145872, TA145873, TA145874, TA145875, TA145876, TA145877, TA145878, TA145879, TA145880, TA145881 311015..410760 0.004 View NZ_CP047304 Vibrio cholerae O1 biovar El Tor strain E7946 Integron TA145883, TA145884, TA145885, TA145886, TA145887, TA145888, TA145889, TA145890, TA145891, TA145892, TA145893, TA145894, TA145895, TA145896, TA145897, TA145898, TA145899, TA145900, TA145901 312112..436375 0 View NZ_CP064351 Vibrio cholerae C6706 Integron TA180865, TA180866, TA180867, TA180868, TA180869, TA180870, TA180871, TA180872, TA180873, TA180874, TA180875, TA180876, TA180877, TA180878, TA180879, TA180880, TA180881, TA180882 378284..502088 0 View NZ_CP072848 Vibrio cholerae strain A1552 Integron TA198989, TA198978, TA198979, TA198980, TA198981, TA198982, TA198983, TA198984, TA198985, TA198986, TA198987, TA198988, TA198990, TA198991, TA198992, TA198993, TA198994 311994..435799 0 View NZ_CP072850 Vibrio cholerae MO10 Integron TA198998, TA198999, TA199000, TA199001, TA199002, TA199003, TA199004, TA199005, TA199006, TA199007, TA199008, TA199009, TA199010, TA199011, TA199012, TA199013 312008..401813 0.005 View NZ_CP080466 Vibrio cholerae strain ICDC-VC3741 Integron TA211906, TA211907, TA211908, TA211909, TA211910, TA211911, TA211912, TA211913, TA211914, TA211915, TA211916, TA211917, TA211918, TA211919, TA211920, TA211921, TA211922 933056..1032792 0.004 View NZ_CP090379 Vibrio cholerae strain BY369 Integron TA230515, TA230516, TA230517, TA230518, TA230519, TA230520, TA230521, TA230522, TA230523, TA230524, TA230525, TA230526, TA230527, TA230528, TA230529, TA230530 309725..399516 0.005 View NZ_CP090400 Vibrio cholerae strain PanChol Integron TA230635, TA230636, TA230637, TA230638, TA230639, TA230640, TA230641, TA230642, TA230643, TA230644, TA230645, TA230646, TA230647, TA230648, TA230649, TA230650, TA230651 836019..937068 0.004 View NZ_CP102928 Vibrio cholerae strain N1252 Integron TA254465, TA254466, TA254467, TA254468, TA254469, TA254470, TA254471, TA254472, TA254473, TA254474, TA254475, TA254476, TA254477, TA254478, TA254479, TA254480, TA254481 718932..819981 0.004 View NZ_LT906615 Vibrio cholerae O1 biovar El Tor str. N16961 Integron TA293792, TA293793, TA293794, TA293795, TA293796, TA293797, TA293798, TA293799, TA293800, TA293801, TA293802, TA293803, TA293804, TA293805, TA293806, TA293807, TA293808, TA293809 309746..435385 0 View NZ_LT907990 Vibrio cholerae strain A19 Integron TA293821, TA293822, TA293823, TA293824, TA293825, TA293826, TA293827, TA293828, TA293829, TA293830, TA293831, TA293832, TA293833, TA293834, TA293835, TA293836, TA293837, TA293838 309746..435385 0 View NZ_LT992487 Vibrio cholerae strain 4295STDY6534216 Integron TA294348, TA294349, TA294350, TA294351, TA294352, TA294353, TA294354, TA294355, TA294356, TA294357, TA294358, TA294359, TA294360, TA294361, TA294362, TA294363, TA294364 897901..987709 0.005 View NZ_LT992489 Vibrio cholerae strain 4295STDY6534232 Integron TA294368, TA294369, TA294370, TA294371, TA294372, TA294373, TA294374, TA294375, TA294376, TA294377, TA294378, TA294379, TA294380, TA294381, TA294382, TA294383, TA294384 379701..469509 0.005 View NZ_LT992493 Vibrio cholerae strain 4295STDY6534248 Integron TA294406, TA294407, TA294408, TA294409, TA294410, TA294411, TA294412, TA294413, TA294414, TA294415, TA294416, TA294417, TA294418, TA294419, TA294420, TA294421, TA294422 202848..292656 0.005 View NZ_OW443148 Vibrio cholerae strain CNRVC190243 isolate YE-NCPHL-19014-PI Integron TA295386, TA295387, TA295388, TA295389, TA295390, TA295391, TA295392, TA295393, TA295394, TA295395, TA295396, TA295397, TA295398, TA295399, TA295400, TA295401, TA295402 354985..456034 0.004	inside	Integron	catB9	-	309750..435387	125637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10406.11 Da
• Isoelectric Point: 9.5084

>T166 WP_000277238.1 NC_002506:c328287-328018 [Vibrio cholerae O1 biovar El Tor str. N16961]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Nucleotide

• Length: 270 bp

>T166 NC_002506:c328287-328018 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGTGATTTATTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10088.58 Da
• Isoelectric Point: 6.0728

>AT166 WP_001258569.1 NC_002506:c328558-328280 [Vibrio cholerae O1 biovar El Tor str. N16961]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Nucleotide

• Length: 279 bp

>AT166 NC_002506:c328558-328280 [Vibrio cholerae O1 biovar El Tor str. N16961]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATAAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10095	Clostridium difficile R20291	47.778	100	0.483
T6286	Campylobacter jejuni subsp. jejuni IA3902	47.778	100	0.483
T28	Helicobacter pylori 26695	44.944	100	0.455
T6127	Helicobacter pylori 26695	41.758	100	0.427
T10181	Aggregatibacter actinomycetemcomitans D11S 1	44.578	94.318	0.42
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	41.111	100	0.416
T10088	Helicobacter pylori P12	39.13	100	0.404
T10070	Tetragenococcus halophilus NBRC 12172	38.636	98.876	0.382
T665	Streptococcus mutans UA159	35.165	100	0.36
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	31.633	100	0.348
T1038	Escherichia coli str. K-12 substr. MG1655	35.632	97.753	0.348
T6290	Escherichia coli BW25113	35.632	97.753	0.348
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	30.612	100	0.337
T10136	Pseudomonas savastanoi	33.333	97.753	0.326
T10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	30	100	0.303

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10136	Pseudomonas savastanoi	35	86.957	0.304

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A151JGK8

Antitoxin

• AlphaFold DB: A0A151KTP9

References

(1) Naeem Iqbal et al. (2015) Comprehensive Functional Analysis of the 18 Vibrio cholerae N16961 Toxin-Antitoxin Systems Substantiates Their Role in Stabilizing the Superintegron. Journal of Bacteriology 197(13):2150-9. [PubMed:25897030]


 experimental literature