10070

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/-
Location 523515..524034 Replicon chromosome
Accession NC_016052
Organism Tetragenococcus halophilus NBRC 12172

Toxin (Protein)


Gene name yafQ Uniprot ID A0A2H6D1U4
Locus tag TEH_RS02455 Protein ID WP_014123908.1
Coordinates 523750..524034 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A2H6CTG1
Locus tag TEH_RS02450 Protein ID WP_014123907.1
Coordinates 523515..523760 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TEH_RS02430 (TEH_05070) 519235..520593 + 1359 WP_014123903.1 transposase -
TEH_RS02435 (TEH_05080) 520709..521356 - 648 WP_014123904.1 pyroglutamyl-peptidase I -
TEH_RS02440 (TEH_05090) 521683..522603 - 921 WP_014123905.1 DUF979 domain-containing protein -
TEH_RS02445 (TEH_05100) 522587..523294 - 708 WP_014123906.1 DUF969 domain-containing protein -
TEH_RS02450 (TEH_05110) 523515..523760 + 246 WP_014123907.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
TEH_RS02455 (TEH_05120) 523750..524034 + 285 WP_014123908.1 type II toxin-antitoxin system YafQ family toxin Toxin
TEH_RS02460 (TEH_05130) 524283..526148 + 1866 WP_014123909.1 beta-glucoside-specific PTS transporter subunit IIABC -
TEH_RS02465 (TEH_05140) 526170..527642 + 1473 WP_014123910.1 glycoside hydrolase family 1 protein -
TEH_RS02470 (TEH_05150) 527664..528503 + 840 WP_014123911.1 PRD domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(11-93)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-65)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11711.52 Da        Isoelectric Point: 10.0281

>T10070 WP_014123908.1 NC_016052:523750-524034 [Tetragenococcus halophilus NBRC 12172]
MKNNPKYKPAFERKFKKHYKNMLKGGRYKKEDFEKVYWKLLYDEQLEPRYNDHPLVNRKPERDLHIKPDWLLIYKYDGEF
VRFIDTGTHADLFK

Download         Length: 285 bp

>T10070 NC_016052:523750-524034 [Tetragenococcus halophilus NBRC 12172]
ATGAAGAATAATCCTAAATATAAGCCAGCTTTTGAGAGAAAATTTAAGAAGCATTATAAAAATATGCTTAAGGGTGGTCG
TTATAAAAAAGAGGACTTTGAAAAAGTTTATTGGAAACTTTTATATGATGAACAACTGGAACCAAGATACAACGATCATC
CTTTGGTAAATCGAAAACCTGAACGTGATTTGCATATCAAGCCAGATTGGTTGTTGATTTATAAATACGATGGTGAGTTT
GTCCGGTTTATCGATACGGGCACTCATGCCGATCTCTTTAAATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9519.59 Da        Isoelectric Point: 4.1475

>AT10070 WP_014123907.1 NC_016052:523515-523760 [Tetragenococcus halophilus NBRC 12172]
MTNQVHFRIDEEDKEKFRIVMQRVGLEPNEAYRIFVKRAIEVGGIPFEVSDPSSQLEEAIKSQDYIEFEDGKEGLDWLNE
E

Download         Length: 246 bp

>AT10070 NC_016052:523515-523760 [Tetragenococcus halophilus NBRC 12172]
ATGACGAATCAAGTACACTTTAGGATAGATGAAGAGGATAAAGAAAAGTTTAGAATTGTGATGCAACGTGTAGGTTTAGA
GCCTAACGAAGCTTATCGGATCTTTGTTAAAAGGGCAATCGAAGTTGGAGGAATCCCGTTCGAAGTTTCGGATCCATCTT
CTCAATTAGAGGAGGCCATTAAAAGCCAGGACTATATTGAATTTGAAGACGGTAAGGAAGGCTTGGATTGGTTAAATGAA
GAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H6D1U4

Antitoxin

Source ID Structure
AlphaFold DB A0A2H6CTG1

References

(1) Xiaotong Luo et al. (2021) Characterization of DinJ-YafQ toxin-antitoxin module in Tetragenococcus halophilus: activity, interplay, and evolution. Applied Microbiology And Biotechnology 105(9):3659-3672. [PubMed:33877415] experimental


experimental experimental literature