Detailed information of TA system TA006289

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 1181402..1181998
• Replicon: chromosome
• Accession: NC_014169
• Organism: Bifidobacterium longum subsp. longum JDM301

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: BLJ_RS05005
• Protein ID: WP_003831807.1
• Coordinates: 1181402..1181695 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7U4H5P9
• Locus tag: BLJ_RS05010
• Protein ID: WP_038425943.1
• Coordinates: 1181699..1181968 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLJ_RS04975 (BLJ_0984)	1176838..1177494	+	657	WP_013140724.1	ATP-binding cassette domain-containing protein	-
BLJ_RS04980 (BLJ_0985)	1177497..1178225	+	729	WP_013140725.1	hypothetical protein	-
BLJ_RS04985	1178222..1178845	+	624	WP_016462919.1	hypothetical protein	-
BLJ_RS04990 (BLJ_0987)	1179049..1179924	+	876	WP_013140727.1	EamA family transporter	-
BLJ_RS04995 (BLJ_0988)	1180048..1180410	+	363	WP_013140728.1	hypothetical protein	-
BLJ_RS05005 (BLJ_0989)	1181402..1181695	-	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BLJ_RS05010 (BLJ_0990)	1181699..1181968	-	270	WP_038425943.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLJ_RS05015 (BLJ_0991)	1182153..1183322	-	1170	WP_013140730.1	ABC transporter permease	-
BLJ_RS05020 (BLJ_0992)	1183319..1183999	-	681	WP_013140731.1	ABC transporter ATP-binding protein	-
BLJ_RS05025 (BLJ_0993)	1183996..1184886	-	891	WP_013140732.1	hypothetical protein	-
BLJ_RS05030 (BLJ_0994)	1184883..1185398	-	516	WP_013140733.1	hypothetical protein	-
BLJ_RS05035 (BLJ_0995)	1185524..1186219	+	696	WP_003829410.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP008885 Bifidobacterium longum strain BXY01 Genomic island TA047845 1153427..1196819 0	inside	Genomic island	-	-	1151969..1195361	43392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T6289 WP_003831807.1 NC_014169:c1181695-1181402 [Bifidobacterium longum subsp. longum JDM301]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T6289 NC_014169:c1181695-1181402 [Bifidobacterium longum subsp. longum JDM301]
ATGCTCAAACCGGATTACACAGCGGCATTCCAGCGAGATATCAAGAAACTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9830.14 Da
• Isoelectric Point: 5.0790

>AT6289 WP_038425943.1 NC_014169:c1181968-1181699 [Bifidobacterium longum subsp. longum JDM301]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVRERRIPLNLSYTTVPPATMEALDEAKNRLADGRPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT6289 NC_014169:c1181968-1181699 [Bifidobacterium longum subsp. longum JDM301]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAGGCGCATTCCCCTAAACCTTTCCTATACGA
CCGTGCCGCCTGCAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCGACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6257	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	100	100	1
T10190	Pseudomonas sp. strain ANT_J3	37.755	100	0.381
T28	Helicobacter pylori 26695	34.737	100	0.375
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	36.458	98.969	0.361
T10095	Clostridium difficile R20291	36.17	97.917	0.354
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	35.417	98.969	0.351
T665	Streptococcus mutans UA159	31.633	100	0.337
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	32.258	96.875	0.312
T6127	Helicobacter pylori 26695	28.866	100	0.311
T10070	Tetragenococcus halophilus NBRC 12172	29.897	100	0.309

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6257	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	96.629	100	0.966
AT10190	Pseudomonas sp. strain ANT_J3	34.524	96.552	0.333
AT10181	Aggregatibacter actinomycetemcomitans D11S 1	34.524	94.382	0.326
AT10136	Pseudomonas savastanoi	32.927	92.135	0.303

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: W6EX94

Antitoxin

• AlphaFold DB: A0A7U4H5P9

References

(1) Yanxia Wei et al. (2016) Functional characterization of RelBE toxin-antitoxin system in probiotic Bifidobacterium longum JDM301. Acta Biochimica Et Biophysica Sinica 48(8):741-9. [PubMed:27451444]


 experimental literature