Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 946345..946969 | Replicon | chromosome |
| Accession | NC_000915 | ||
| Organism | Helicobacter pylori 26695 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O25554 |
| Locus tag | HP_RS04360 | Protein ID | WP_000916169.1 |
| Coordinates | 946345..946611 (-) | Length | 89 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | O25555 |
| Locus tag | HP_RS04365 | Protein ID | WP_001134075.1 |
| Coordinates | 946592..946969 (-) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HP_RS04330 (HP0888) | 942347..943114 | - | 768 | WP_000242349.1 | ABC transporter ATP-binding protein | - |
| HP_RS04335 (HP0889) | 943114..944094 | - | 981 | WP_000921458.1 | iron ABC transporter permease | - |
| HP_RS04340 (HP0890) | 944087..944935 | - | 849 | WP_000504124.1 | SDR family oxidoreductase | - |
| HP_RS04345 (HP0891) | 945075..945599 | + | 525 | WP_001135603.1 | acyl-CoA thioesterase | - |
| HP_RS04350 (HP0892) | 945691..945963 | - | 273 | WP_000955518.1 | type II toxin-antitoxin system mRNA interferase toxin, HP0892 family | - |
| HP_RS04355 (HP0893) | 945977..946264 | - | 288 | WP_001134157.1 | type II toxin-antitoxin system antitoxin | - |
| HP_RS04360 (HP0894) | 946345..946611 | - | 267 | WP_000916169.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | Toxin |
| HP_RS04365 (HP0895) | 946592..946969 | - | 378 | WP_001134075.1 | hypothetical protein | Antitoxin |
| HP_RS04370 (HP0896) | 947580..949706 | - | 2127 | WP_000753173.1 | Hop family adhesin BabB | - |
| HP_RS04385 | 950510..950773 | - | 264 | WP_015056067.1 | hypothetical protein | - |
| HP_RS04390 (HP0898) | 950740..951852 | - | 1113 | WP_000109824.1 | hydrogenase formation protein HypD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 89 a.a. Molecular weight: 10344.18 Da Isoelectric Point: 8.0602
>T28 WP_000916169.1 NC_000915:c946611-946345 [Helicobacter pylori 26695]
VLKLNLKKSFQKDFDKLLLNGFDDSVLNEVILTLRKKEPLDPQFQDHALKGKWKPFRECHIKPDVLLVYLVKDDELILLR
LGSHSELF
VLKLNLKKSFQKDFDKLLLNGFDDSVLNEVILTLRKKEPLDPQFQDHALKGKWKPFRECHIKPDVLLVYLVKDDELILLR
LGSHSELF
Download Length: 267 bp
>T28 NC_000915:c946611-946345 [Helicobacter pylori 26695]
GTGTTGAAGCTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTGCTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCCTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATTAAGCCTGATGTTTTGCTTGTGTATTTAGTGAAAGATGATGAACTGATTTTGTTAAGG
TTAGGCAGTCATAGCGAGCTGTTTTAA
GTGTTGAAGCTCAATCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTGCTTTTGAATGGGTTTGATGATAGCGTTTT
GAATGAAGTCATTCTAACCTTAAGAAAAAAAGAACCGCTAGATCCACAATTTCAAGATCATGCCTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATTAAGCCTGATGTTTTGCTTGTGTATTTAGTGAAAGATGATGAACTGATTTTGTTAAGG
TTAGGCAGTCATAGCGAGCTGTTTTAA
Antitoxin
Download Length: 126 a.a. Molecular weight: 14713.74 Da Isoelectric Point: 9.0068
>AT28 WP_001134075.1 NC_000915:c946969-946592 [Helicobacter pylori 26695]
MPNTTAKKDYTKYSKKQLFNLIHQLERKIKKMQNDRISFKEKMAKELEKRDQNFKDKIDALNELLQKISQAFDDKRDCCL
GHEIPNIETQQAMRDVGNKETDLIVEDFSSYSNERKRALGVEAQS
MPNTTAKKDYTKYSKKQLFNLIHQLERKIKKMQNDRISFKEKMAKELEKRDQNFKDKIDALNELLQKISQAFDDKRDCCL
GHEIPNIETQQAMRDVGNKETDLIVEDFSSYSNERKRALGVEAQS
Download Length: 378 bp
>AT28 NC_000915:c946969-946592 [Helicobacter pylori 26695]
ATGCCTAACACCACCGCCAAAAAAGACTACACAAAATACAGCAAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAATTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGTCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCATGAGATCCCAAACATTGAAACGCAACAAGCCATGAGAGATGTAGGTAACAAAGAGACAGATTTGATTGTTGAGGA
TTTTTCTAGTTACAGCAATGAAAGAAAAAGGGCTTTAGGTGTTGAAGCTCAATCTTAA
ATGCCTAACACCACCGCCAAAAAAGACTACACAAAATACAGCAAAAAACAGCTTTTTAATTTAATCCATCAATTAGAGCG
AAAAATCAAAAAGATGCAAAATGATAGAATTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGAAAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCGTTAAATGAACTCTTGCAAAAAATCAGTCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCATGAGATCCCAAACATTGAAACGCAACAAGCCATGAGAGATGTAGGTAACAAAGAGACAGATTTGATTGTTGAGGA
TTTTTCTAGTTACAGCAATGAAAGAAAAAGGGCTTTAGGTGTTGAAGCTCAATCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 1Z8M | |
| PDB | 4LSY | |
| PDB | 4LTT | |
| PDB | 4LS4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2J9KIQ1 |
References
(1) Ae-Ran Kwon et al. (2012) Structural and biochemical characterization of HP0315 from Helicobacter pylori as a VapD protein with an endoribonuclease activity. Nucleic Acids Research 40(9):4216-28. [PubMed:22241770]
(2) Chinar Pathak et al. (2013) Crystal structure of apo and copper bound HP0894 toxin from Helicobacter pylori 26695 and insight into mRNase activity. Biochimica Et Biophysica Acta 1834(12):2579-90. [PubMed:24060809]
(3) Julija Armalyte et al. (2012) Characterization of Escherichia coli dinJ-yafQ toxin-antitoxin system using insights from mutagenesis data. Journal of Bacteriology 194(6):1523-32. [PubMed:22247505]
(4) Kyung-Doo Han et al. (2011) Functional identification of toxin-antitoxin molecules from Helicobacter pylori 26695 and structural elucidation of the molecular interactions. The Journal of Biological Chemistry 286(6):4842-53. [PubMed:21123184]