Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 513085..513570 Replicon chromosome
Accession NC_017491
Organism Lactobacillus rhamnosus ATCC 8530

Toxin (Protein)


Gene name relE Uniprot ID C2K0Y1
Locus tag LRHK_RS13845 Protein ID WP_005691636.1
Coordinates 513085..513270 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A508YYT1
Locus tag LRHK_RS02420 Protein ID WP_014571130.1
Coordinates 513298..513570 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRHK_RS02395 (LRHK_518) 508092..508781 + 690 WP_005691628.1 rhomboid family intramembrane serine protease -
LRHK_RS02400 (LRHK_520) 509273..509881 - 609 WP_005691630.1 hypothetical protein -
LRHK_RS02410 (LRHK_522) 510328..511203 + 876 WP_024305787.1 XRE family transcriptional regulator -
LRHK_RS02415 (LRHK_523) 511446..512861 - 1416 WP_005691634.1 DNA polymerase III subunit epsilon -
LRHK_RS13845 (LRHK_524) 513085..513270 - 186 WP_005691636.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
LRHK_RS02420 (LRHK_525) 513298..513570 - 273 WP_014571130.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LRHK_RS02425 (LRHK_526) 514132..514380 + 249 WP_005691639.1 hypothetical protein -
LRHK_RS02430 (LRHK_527) 514623..514916 + 294 WP_005684269.1 metalloregulator ArsR/SmtB family transcription factor -
LRHK_RS02435 (LRHK_528) 514998..516161 + 1164 WP_005691640.1 MFS transporter -
LRHK_RS02440 (LRHK_529) 516315..516890 + 576 WP_014571131.1 DNA-3-methyladenine glycosylase I -
LRHK_RS02445 (LRHK_530) 517033..517629 + 597 WP_005691643.1 IS607 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 509273..519295 10022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-57)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-65)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6781.86 Da        Isoelectric Point: 7.4698

>T6239 WP_005691636.1 NC_017491:c513270-513085 [Lactobacillus rhamnosus ATCC 8530]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Download         Length: 186 bp

>T6239 NC_017491:c513270-513085 [Lactobacillus rhamnosus ATCC 8530]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10005.62 Da        Isoelectric Point: 9.5939

>AT6239 WP_014571130.1 NC_017491:c513570-513298 [Lactobacillus rhamnosus ATCC 8530]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Download         Length: 273 bp

>AT6239 NC_017491:c513570-513298 [Lactobacillus rhamnosus ATCC 8530]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.


Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2J6C9

Antitoxin

Source ID Structure
AlphaFold DB A0A508YYT1

References

(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113] experimental


experimental experimental literature