Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/- |
Location | 1..565 | Replicon | chromosome |
Accession | MK544943 | ||
Organism | Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes |
Toxin (Protein)
Gene name | yafQ | Uniprot ID | - |
Locus tag | Locus_1 | Protein ID | QIP66198.1 |
Coordinates | 263..565 (+) | Length | 101 a.a. |
Antitoxin (Protein)
Gene name | dinJ | Uniprot ID | - |
Locus tag | Locus_0 | Protein ID | QIP66197.1 |
Coordinates | 1..270 (+) | Length | 90 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
Locus_0 | 1..270 | + | 270 | QIP66197.1 | DinJ | Antitoxin |
Locus_1 | 263..565 | + | 303 | QIP66198.1 | YafQ | Toxin |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 101 a.a. Molecular weight: 11383.23 Da Isoelectric Point: 10.4225
>T10112 QIP66198.1 MK544943:263-565 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGGWLLIYKIK
QQDLILTLVRTGSHHNLLGK
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGGWLLIYKIK
QQDLILTLVRTGSHHNLLGK
Download Length: 303 bp
>T10112 MK544943:263-565 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGGCTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGGTAAATAG
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGGCTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGGTAAATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 9996.71 Da Isoelectric Point: 7.2026
>AT10112 QIP66197.1 MK544943:1-270 [Lacticaseibacillus paracasei]
MAATKKETRLNIRIDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV
MAATKKETRLNIRIDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV
Download Length: 270 bp
>AT10112 MK544943:1-270 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTATTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAGGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACACCT
GATGACATGTGGAGAGACTTGAATGTATAG
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTATTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAGGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACACCT
GATGACATGTGGAGAGACTTGAATGTATAG
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT10111 | Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes |
98.876 |
100 |
0.989 |
AT665 | Streptococcus mutans UA159 |
36.905 |
94.382 |
0.348 |
AT6144 | Lactobacillus rhamnosus ATCC 8530 |
34.146 |
92.135 |
0.315 |
AT6239 | Lactobacillus rhamnosus ATCC 8530 |
34.146 |
92.135 |
0.315 |
AT10110 | Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes |
34.146 |
92.135 |
0.315 |
AT6139 | Lactobacillus rhamnosus Lc 705 |
34.146 |
92.135 |
0.315 |
AT6256 | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 |
31.034 |
97.753 |
0.303 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|
Antitoxin
Source | ID | Structure |
---|
References
(1) Alessia Levante et al. (2019) Expression of DinJ-YafQ System of Lactobacillus casei Group Strains in Response to Food Processing Stresses. Microorganisms 7(10):438. [PubMed:31614503]
(2) Alberto Ferrari et al. (2019) Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest. Scientific Reports 9(1):7645. [PubMed:31114007]
experimental literature