Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | relBE/RHH-RelE |
Location | 512909..513394 | Replicon | chromosome |
Accession | NC_013199 | ||
Organism | Lactobacillus rhamnosus Lc 705 |
Toxin (Protein)
Gene name | relE | Uniprot ID | C2K0Y1 |
Locus tag | LC705_RS14280 | Protein ID | WP_005691636.1 |
Coordinates | 512909..513094 (-) | Length | 62 a.a. |
Antitoxin (Protein)
Gene name | relB | Uniprot ID | A0A508YYT1 |
Locus tag | LC705_RS02415 | Protein ID | WP_014571130.1 |
Coordinates | 513122..513394 (-) | Length | 91 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
LC705_RS02390 (LC705_00502) | 507916..508605 | + | 690 | WP_005691628.1 | rhomboid family intramembrane serine protease | - |
LC705_RS02395 (LC705_00504) | 509097..509705 | - | 609 | WP_005691630.1 | hypothetical protein | - |
LC705_RS02405 (LC705_00506) | 510152..511027 | + | 876 | WP_024305787.1 | XRE family transcriptional regulator | - |
LC705_RS02410 (LC705_00507) | 511270..512685 | - | 1416 | WP_005691634.1 | DNA polymerase III subunit epsilon | - |
LC705_RS14280 (LC705_00508) | 512909..513094 | - | 186 | WP_005691636.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | Toxin |
LC705_RS02415 (LC705_00509) | 513122..513394 | - | 273 | WP_014571130.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
LC705_RS02420 (LC705_00510) | 513956..514204 | + | 249 | WP_005691639.1 | hypothetical protein | - |
LC705_RS02425 (LC705_00511) | 514447..514740 | + | 294 | WP_005684269.1 | metalloregulator ArsR/SmtB family transcription factor | - |
LC705_RS02430 (LC705_00512) | 514822..515985 | + | 1164 | WP_005691640.1 | MFS transporter | - |
LC705_RS02435 (LC705_00513) | 516139..516714 | + | 576 | WP_014571131.1 | DNA-3-methyladenine glycosylase I | - |
LC705_RS02440 (LC705_00514) | 516857..517453 | + | 597 | WP_005691643.1 | IS607 family transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
inside | Genomic island | - | - | 509097..519119 | 10022 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 62 a.a. Molecular weight: 6781.86 Da Isoelectric Point: 7.4698
>T6139 WP_005691636.1 NC_013199:c513094-512909 [Lactobacillus rhamnosus Lc 705]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE
Download Length: 186 bp
>T6139 NC_013199:c513094-512909 [Lactobacillus rhamnosus Lc 705]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10005.62 Da Isoelectric Point: 9.5939
>AT6139 WP_014571130.1 NC_013199:c513394-513122 [Lactobacillus rhamnosus Lc 705]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD
Download Length: 273 bp
>AT6139 NC_013199:c513394-513122 [Lactobacillus rhamnosus Lc 705]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT6239 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
AT10110 | Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes |
100 |
100 |
1 |
AT6144 | Lactobacillus rhamnosus ATCC 8530 |
100 |
100 |
1 |
AT10109 | Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes |
90 |
100 |
0.9 |
AT10108 | Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes |
75.556 |
100 |
0.756 |
AT10136 | Pseudomonas savastanoi |
33.333 |
96.667 |
0.322 |
AT10112 | Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes |
34.146 |
92.135 |
0.315 |
AT10111 | Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes |
32.927 |
92.135 |
0.303 |
AT665 | Streptococcus mutans UA159 |
36 |
83.333 |
0.3 |
Multiple sequence alignment
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7X2J6C9 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A508YYT1 |
References
(1) K M Klimina et al. (2013) Identification and characterization of toxin-antitoxin systems in strains of Lactobacillus rhamnosus isolated from humans. Anaerobe 22:82-9. [PubMed:23727113]
experimental literature