Detailed information of TA system TA000665

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH-RelE
• Location: 849954..850491
• Replicon: chromosome
• Accession: NC_004350
• Organism: Streptococcus mutans UA159

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8DUM3
• Locus tag: SMU_RS04160
• Protein ID: WP_002262889.1
• Coordinates: 850213..850491 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q8DUM4
• Locus tag: SMU_RS04155
• Protein ID: WP_002262888.1
• Coordinates: 849954..850226 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMU_RS04140 (SMU.891)	845296..846900	+	1605	WP_002262884.1	type I restriction-modification system subunit M	-
SMU_RS04145 (SMU.892)	846893..848704	+	1812	WP_002352261.1	restriction endonuclease subunit S	-
SMU_RS04150 (SMU.893)	848735..849880	+	1146	WP_002262887.1	AAA family ATPase	-
SMU_RS04155 (SMU.895)	849954..850226	+	273	WP_002262888.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SMU_RS04160 (SMU.896)	850213..850491	+	279	WP_002262889.1	type II toxin-antitoxin system YafQ family toxin	Toxin
SMU_RS04165 (SMU.897)	850494..853541	+	3048	WP_002262890.1	type I restriction endonuclease subunit R	-
SMU_RS04170 (SMU.898)	853755..854129	+	375	WP_002262891.1	DUF1149 family protein	-
SMU_RS04175 (SMU.899)	854135..854980	+	846	WP_002262892.1	DegV family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-76)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10722.79 Da
• Isoelectric Point: 10.2475

>T665 WP_002262889.1 NC_004350:850213-850491 [Streptococcus mutans UA159]
MLKIKQTRQFKKSLKKVVKQGKDINKLFAIVELLCQKSELPLALRNHELKGRWRGIRELHIESDWLLAYQVLDDELVLLL
IDTGSHAQMLGM

Nucleotide

• Length: 279 bp

>T665 NC_004350:850213-850491 [Streptococcus mutans UA159]
ATGCTAAAGATTAAGCAAACCAGACAATTTAAGAAATCCTTAAAAAAGGTAGTAAAACAAGGAAAAGATATTAACAAGCT
TTTTGCTATCGTAGAATTATTATGCCAAAAATCAGAACTTCCCTTAGCATTGAGAAATCATGAGTTGAAGGGAAGGTGGA
GAGGCATTAGAGAACTTCATATTGAATCAGACTGGCTGTTAGCCTATCAAGTTTTGGATGACGAACTTGTCTTACTCTTG
ATTGATACAGGTAGTCACGCACAGATGTTAGGAATGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10051.41 Da
• Isoelectric Point: 4.4482

>AT665 WP_002262888.1 NC_004350:849954-850226 [Streptococcus mutans UA159]
MVVKNKANINIKIDLEDKAAADAIFAHMGLTTSAAVNMFIKRVIDDQALPFTPRVKNTLDIALEQAKNDDVETFDSFDDW
KSEMSAYAKD

Nucleotide

• Length: 273 bp

>AT665 NC_004350:849954-850226 [Streptococcus mutans UA159]
ATGGTTGTAAAAAATAAAGCAAATATCAATATCAAAATTGATTTGGAAGATAAAGCAGCAGCGGATGCTATTTTTGCTCA
CATGGGTCTAACAACAAGTGCTGCTGTTAATATGTTTATTAAACGTGTCATTGATGATCAAGCTCTGCCATTTACTCCAA
GAGTAAAAAATACTTTGGACATTGCTTTAGAGCAGGCTAAAAATGATGATGTTGAAACTTTTGATAGTTTTGATGATTGG
AAATCAGAGATGAGTGCCTATGCTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10095	Clostridium difficile R20291	45.455	95.652	0.435
T10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	41.935	100	0.424
T28	Helicobacter pylori 26695	37.778	100	0.386
T10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	46	81.967	0.377
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	34.343	100	0.37
T6127	Helicobacter pylori 26695	36.667	100	0.367
T166	Vibrio cholerae O1 biovar El Tor str. N16961	35.165	100	0.36
T6290	Escherichia coli BW25113	37.079	96.739	0.359
T1038	Escherichia coli str. K-12 substr. MG1655	37.079	96.739	0.359
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	33.333	100	0.359
T6144	Lactobacillus rhamnosus ATCC 8530	42	81.967	0.344
T6139	Lactobacillus rhamnosus Lc 705	42	81.967	0.344
T6239	Lactobacillus rhamnosus ATCC 8530	42	81.967	0.344
T10110	Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes	42	81.967	0.344
T6286	Campylobacter jejuni subsp. jejuni IA3902	36.905	92.308	0.341
T10070	Tetragenococcus halophilus NBRC 12172	38.095	89.362	0.34
T6289	Bifidobacterium longum subsp. longum JDM301	31.633	100	0.337
T6257	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	31.633	100	0.337
T10190	Pseudomonas sp. strain ANT_J3	35.227	95.652	0.337
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	35.227	95.652	0.337
T10136	Pseudomonas savastanoi	34.118	92.391	0.315
T10088	Helicobacter pylori P12	31.818	94.624	0.301

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	36.905	94.382	0.348
AT10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	35.714	94.382	0.337
AT6239	Lactobacillus rhamnosus ATCC 8530	36	83.333	0.3
AT10110	Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes	36	83.333	0.3
AT6139	Lactobacillus rhamnosus Lc 705	36	83.333	0.3
AT6144	Lactobacillus rhamnosus ATCC 8530	36	83.333	0.3

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q8DUM3

Antitoxin

• AlphaFold DB: Q8DUM4

References

(1) Xiao-Lin Tian et al. (2019) ClpP is required for proteolytic regulation of type II toxin-antitoxin systems and persister cell formation in Streptococcus mutans. Access Microbiology 1(8):e000054. [PubMed:32974554]


 experimental literature