Detailed information of TA system TA010190

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 2228..2795
• Replicon: plasmid pA3J1
• Accession: NZ_KY498026
• Organism: Pseudomonas sp. strain ANT_J3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HXI37_RS00015
• Protein ID: WP_176704754.1
• Coordinates: 2228..2572 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HXI37_RS00020
• Protein ID: WP_176704758.1
• Coordinates: 2532..2795 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXI37_RS00005 (pA3J1_p01)	1..1236	-	1236	WP_176704752.1	TRAFs-binding domain-containing protein	-
HXI37_RS00010 (pA3J1_p02)	1610..2224	+	615	WP_176704753.1	recombinase family protein	-
HXI37_RS00015 (pA3J1_p03)	2228..2572	-	345	WP_176704754.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HXI37_RS00020 (pA3J1_p04)	2532..2795	-	264	WP_176704758.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HXI37_RS00025 (pA3J1_p05)	3216..3860	+	645	WP_176704755.1	AAA family ATPase	-
HXI37_RS00030 (pA3J1_p06)	3853..4131	+	279	WP_176704756.1	hypothetical protein	-
HXI37_RS00035 (pA3J1_p07)	4295..5041	+	747	WP_176704746.1	replication initiation protein	-
HXI37_RS00040 (pA3J1_p08)	5888..6199	-	312	WP_176704747.1	hypothetical protein	-
HXI37_RS00045 (pA3J1_p09)	6196..6519	-	324	WP_255255456.1	hypothetical protein	-
HXI37_RS00050 (pA3J1_p10)	6656..7765	+	1110	WP_176704748.1	MobQ family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9794	9794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (22-113)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12770.54 Da
• Isoelectric Point: 8.5069

>T10190 WP_176704754.1 NZ_KY498026:c2572-2228 [Pseudomonas sp.]
MSCSTNLKKTAASKRASLPRAADYTKSFIKDWERLSRSGRYDMNRLKEAMLLLIANDAPLGAEWLDHPLKGDWAGHRECH
VGGDFLLAYKTDDSGKTGLIIFVRTGTHAELFNE

Nucleotide

• Length: 345 bp

>T10190 NZ_KY498026:c2572-2228 [Pseudomonas sp.]
ATGAGTTGTTCGACGAACTTGAAAAAAACAGCCGCCAGTAAACGAGCAAGCCTGCCCAGGGCAGCTGATTACACCAAATC
ATTCATCAAAGACTGGGAGCGCCTATCCCGTTCAGGCCGGTACGACATGAACCGGCTCAAAGAAGCCATGCTGCTGCTGA
TTGCTAACGATGCTCCGCTCGGTGCTGAATGGCTCGACCACCCTCTCAAGGGAGACTGGGCAGGGCATCGTGAGTGCCAT
GTCGGTGGCGACTTCCTGCTGGCCTACAAAACCGATGACAGCGGTAAAACGGGTTTAATCATTTTTGTCCGCACCGGCAC
TCACGCTGAACTGTTCAACGAGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9461.86 Da
• Isoelectric Point: 6.4632

>AT10190 WP_176704758.1 NZ_KY498026:c2795-2532 [Pseudomonas sp.]
MLHIRVDDNLKAQATTALGAMGLTMADAVRLLLHRVVADQAFPLELKVPNAATRAAMSEADAIAKARNARFATADELFDE
LEKNSRQ

Nucleotide

• Length: 264 bp

>AT10190 NZ_KY498026:c2795-2532 [Pseudomonas sp.]
ATGCTGCACATCCGAGTTGACGACAACCTCAAAGCGCAAGCAACCACAGCGCTTGGAGCTATGGGGCTTACAATGGCTGA
CGCTGTTCGTTTGCTTCTACATCGTGTCGTTGCTGATCAGGCGTTCCCGCTCGAGCTTAAAGTCCCGAATGCTGCGACCC
GTGCGGCTATGTCTGAAGCCGATGCAATTGCTAAAGCTCGCAATGCTCGCTTTGCCACTGCAGATGAGTTGTTCGACGAA
CTTGAAAAAAACAGCCGCCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T1038	Escherichia coli str. K-12 substr. MG1655	45.263	100	0.467
T6290	Escherichia coli BW25113	45.263	100	0.467
T10136	Pseudomonas savastanoi	46.154	98.113	0.453
T28	Helicobacter pylori 26695	41.379	98.864	0.409
T10095	Clostridium difficile R20291	42.857	94.792	0.406
T6289	Bifidobacterium longum subsp. longum JDM301	37.755	100	0.381
T6257	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	37.755	100	0.381
T6127	Helicobacter pylori 26695	38.636	97.778	0.378
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	37.113	97	0.36
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	36.082	97	0.35
T665	Streptococcus mutans UA159	35.227	95.652	0.337
T10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	35.955	93.684	0.337
T10110	Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes	39.583	78.689	0.311
T6239	Lactobacillus rhamnosus ATCC 8530	39.583	78.689	0.311
T6144	Lactobacillus rhamnosus ATCC 8530	39.583	78.689	0.311
T6139	Lactobacillus rhamnosus Lc 705	39.583	78.689	0.311
T10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	39.583	78.689	0.311
T10191	Synechococcus sp. CB0101	23.009	100	0.31
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	32.222	93.75	0.302
T10088	Helicobacter pylori P12	32.184	93.548	0.301

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT10136	Pseudomonas savastanoi	38.272	93.103	0.356
AT1038	Escherichia coli str. K-12 substr. MG1655	38.961	89.535	0.349
AT6290	Escherichia coli BW25113	38.961	89.535	0.349
AT6289	Bifidobacterium longum subsp. longum JDM301	34.524	96.552	0.333
AT10095	Clostridium difficile R20291	32.941	97.701	0.322

Multiple sequence alignment

References

(1) Krzysztof Romaniuk et al. (2017) Molecular characterization of the pA3J1 plasmid from the psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_J3. Plasmid 92:49-56. [PubMed:28802584]


 experimental literature