Detailed information of TA system TA010110

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/-
• Location: 1..486
• Replicon: chromosome
• Accession: MK544948
• Organism: Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: Locus_1
• Protein ID: QIP66208.1
• Coordinates: 301..486 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: Locus_0
• Protein ID: QIP66207.1
• Coordinates: 1..273 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	1..273	+	273	QIP66207.1	DinJ	Antitoxin
Locus_1	301..486	+	186	QIP66208.1	YafQ	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-57)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-65)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6781.86 Da
• Isoelectric Point: 7.4698

>T10110 QIP66208.1 MK544948:301-486 [Lacticaseibacillus rhamnosus]
MQRQGHVKLASLHDHALKGAHSGERALRVAPDWLLVYKVDAEALILMLLATGTHRDTLNIE

Nucleotide

• Length: 186 bp

>T10110 MK544948:301-486 [Lacticaseibacillus rhamnosus]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10005.62 Da
• Isoelectric Point: 9.5939

>AT10110 QIP66207.1 MK544948:1-273 [Lacticaseibacillus rhamnosus]
MTKKSRISVRIDTKTKERALHVLNSMGLDISSAINMYLKRIGDTGALPFTPPMSFVDQLQVAEADVKAGRIKSFKTVDAL
MKDLYSDVDD

Nucleotide

• Length: 273 bp

>AT10110 MK544948:1-273 [Lacticaseibacillus rhamnosus]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6139	Lactobacillus rhamnosus Lc 705	100	100	1
T6144	Lactobacillus rhamnosus ATCC 8530	100	100	1
T6239	Lactobacillus rhamnosus ATCC 8530	100	100	1
T10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	93.443	100	0.934
T10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	93.443	100	0.934
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	46	81.967	0.377
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	44	81.967	0.361
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	50	68.852	0.344
T665	Streptococcus mutans UA159	42	81.967	0.344
T10095	Clostridium difficile R20291	48.837	70.492	0.344
T10070	Tetragenococcus halophilus NBRC 12172	40.816	80.328	0.328
T28	Helicobacter pylori 26695	44.444	73.77	0.328
T6290	Escherichia coli BW25113	42.222	73.77	0.311
T6286	Campylobacter jejuni subsp. jejuni IA3902	42.222	73.77	0.311
T1038	Escherichia coli str. K-12 substr. MG1655	42.222	73.77	0.311
T10190	Pseudomonas sp. strain ANT_J3	39.583	78.689	0.311

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6139	Lactobacillus rhamnosus Lc 705	100	100	1
AT6144	Lactobacillus rhamnosus ATCC 8530	100	100	1
AT6239	Lactobacillus rhamnosus ATCC 8530	100	100	1
AT10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	90	100	0.9
AT10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	75.556	100	0.756
AT10136	Pseudomonas savastanoi	33.333	96.667	0.322
AT10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	34.146	92.135	0.315
AT10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	32.927	92.135	0.303
AT665	Streptococcus mutans UA159	36	83.333	0.3

Multiple sequence alignment

References

(1) Alessia Levante et al. (2019) Expression of DinJ-YafQ System of Lactobacillus casei Group Strains in Response to Food Processing Stresses. Microorganisms 7(10):438. [PubMed:31614503]

(2) Alberto Ferrari et al. (2019) Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest. Scientific Reports 9(1):7645. [PubMed:31114007]


 experimental literature