Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/- |
| Location | 1..486 | Replicon | chromosome |
| Accession | MK544947 | ||
| Organism | Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | - |
| Locus tag | Locus_1 | Protein ID | QIP66206.1 |
| Coordinates | 301..486 (+) | Length | 62 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | - |
| Locus tag | Locus_0 | Protein ID | QIP66205.1 |
| Coordinates | 1..273 (+) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Locus_0 | 1..273 | + | 273 | QIP66205.1 | DinJ | Antitoxin |
| Locus_1 | 301..486 | + | 186 | QIP66206.1 | YafQ | Toxin |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 62 a.a. Molecular weight: 6787.85 Da Isoelectric Point: 7.5253
>T10108 QIP66206.1 MK544947:301-486 [Lacticaseibacillus rhamnosus]
MQRQAHVKLASLHDHALKGAHSGERALHIAPDWLLVYKVDAEALILKLLATGTHRDTLNIE
MQRQAHVKLASLHDHALKGAHSGERALHIAPDWLLVYKVDAEALILKLLATGTHRDTLNIE
Download Length: 186 bp
>T10108 MK544947:301-486 [Lacticaseibacillus rhamnosus]
ATGCAGCGTCAAGCTCATGTAAAATTAGCTTCTTTACACGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCATATTGCTCCTGATTGGCTTCTGGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAAAGCTTCTTGCAACAGGCACAC
ACCGCGACACATTGAACATTGAGTAA
ATGCAGCGTCAAGCTCATGTAAAATTAGCTTCTTTACACGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCATATTGCTCCTGATTGGCTTCTGGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAAAGCTTCTTGCAACAGGCACAC
ACCGCGACACATTGAACATTGAGTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10261.88 Da Isoelectric Point: 10.2575
>AT10108 QIP66205.1 MK544947:1-273 [Lacticaseibacillus rhamnosus]
MAKESHIRNWINTITKKRALHVLNRLGLERFSAINMYSKRIGDTGALPFTLEMSFADQLRFAEADVKAGRIKSFKTVGAL
MKDLYNDVDD
MAKESHIRNWINTITKKRALHVLNRLGLERFSAINMYSKRIGDTGALPFTLEMSFADQLRFAEADVKAGRIKSFKTVGAL
MKDLYNDVDD
Download Length: 273 bp
>AT10108 MK544947:1-273 [Lacticaseibacillus rhamnosus]
ATGGCAAAAGAATCCCATATCCGCAATTGGATTAACACAATAACCAAAAAACGAGCTCTCCATGTTCTTAATCGTCTGGG
ACTAGAGAGATTCTCGGCTATTAACATGTATTCGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACTTGAAATGT
CATTCGCCGATCAGCTTCGATTTGCAGAAGCAGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTTGGTGCTTTG
ATGAAGGATTTATACAATGATGTTGACGATTGA
ATGGCAAAAGAATCCCATATCCGCAATTGGATTAACACAATAACCAAAAAACGAGCTCTCCATGTTCTTAATCGTCTGGG
ACTAGAGAGATTCTCGGCTATTAACATGTATTCGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACTTGAAATGT
CATTCGCCGATCAGCTTCGATTTGCAGAAGCAGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTTGGTGCTTTG
ATGAAGGATTTATACAATGATGTTGACGATTGA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6144 | Lactobacillus rhamnosus ATCC 8530 |
75.556 |
100 |
0.756 |
| AT10110 | Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes |
75.556 |
100 |
0.756 |
| AT10109 | Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes |
75.556 |
100 |
0.756 |
| AT6139 | Lactobacillus rhamnosus Lc 705 |
75.556 |
100 |
0.756 |
| AT6239 | Lactobacillus rhamnosus ATCC 8530 |
75.556 |
100 |
0.756 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Alessia Levante et al. (2019) Expression of DinJ-YafQ System of Lactobacillus casei Group Strains in Response to Food Processing Stresses. Microorganisms 7(10):438. [PubMed:31614503]
(2) Alberto Ferrari et al. (2019) Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest. Scientific Reports 9(1):7645. [PubMed:31114007]