10095

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/-
Location 2055623..2056188 Replicon chromosome
Accession NC_013316
Organism Clostridium difficile R20291

Toxin (Protein)


Gene name relE Uniprot ID C7H6P5
Locus tag CDR20291_RS09590 Protein ID WP_002596328.1
Coordinates 2055623..2055913 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C7H6P4
Locus tag CDR20291_RS09595 Protein ID WP_005924829.1
Coordinates 2055910..2056188 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDR20291_RS09560 (CDR20291_1753) 2051219..2051680 + 462 WP_012816193.1 hypothetical protein -
CDR20291_RS09565 (CDR20291_1754) 2052125..2052538 + 414 WP_012816194.1 hypothetical protein -
CDR20291_RS09570 (CDR20291_1755) 2052640..2053092 + 453 WP_012816195.1 sigma-24 (feci) -
CDR20291_RS09575 (CDR20291_1756) 2053103..2053564 + 462 WP_009296689.1 sigma-70 family RNA polymerase sigma factor -
CDR20291_RS09580 (CDR20291_1757) 2053593..2053883 + 291 WP_024739841.1 DUF3847 domain-containing protein -
CDR20291_RS09585 (CDR20291_1758) 2054070..2055515 + 1446 WP_104732839.1 MobA/MobL family protein -
CDR20291_RS09590 (CDR20291_1759) 2055623..2055913 - 291 WP_002596328.1 type II toxin-antitoxin system YafQ family toxin Toxin
CDR20291_RS09595 (CDR20291_1760) 2055910..2056188 - 279 WP_005924829.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CDR20291_RS09600 (CDR20291_1761) 2056315..2056713 + 399 WP_012816198.1 cysteine-rich VLP domain-containing protein -
CDR20291_RS09605 (CDR20291_1762) 2056827..2057561 + 735 WP_012816199.1 phage replisome organizer N-terminal domain-containing protein -
CDR20291_RS09610 (CDR20291_1763) 2057558..2058421 + 864 WP_104732840.1 ATP-binding protein -
CDR20291_RS09615 2058465..2058659 + 195 WP_104732841.1 transposon-encoded TnpW family protein -
CDR20291_RS09620 (CDR20291_1764) 2058737..2059102 + 366 WP_104732842.1 hypothetical protein -
CDR20291_RS09625 2059197..2059823 + 627 Protein_1842 TraM recognition domain-containing protein -
CDR20291_RS09630 (CDR20291_1765) 2059876..2060253 + 378 WP_009891894.1 TnpV protein -
CDR20291_RS09635 2060267..2060548 + 282 WP_009891893.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2043677..2141157 97480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-90)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11112.87 Da        Isoelectric Point: 8.0630

>T10095 WP_002596328.1 NC_013316:c2055913-2055623 [Clostridioides difficile R20291]
MKETKYTVKYTTSFKKDYKRAIKRGLKIELLEQVVALLAMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Download         Length: 291 bp

>T10095 NC_013316:c2055913-2055623 [Clostridioides difficile R20291]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10247.66 Da        Isoelectric Point: 4.4692

>AT10095 WP_005924829.1 NC_013316:c2056188-2055910 [Clostridioides difficile R20291]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETVAAMLEAERIAKDPSVKGY
NDLDELFADLKR

Download         Length: 279 bp

>AT10095 NC_013316:c2056188-2055910 [Clostridioides difficile R20291]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6256 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

59.375

100

0.594
T166 Vibrio cholerae O1 biovar El Tor str. N16961

47.778

100

0.483
T6286 Campylobacter jejuni subsp. jejuni IA3902

45.652

100

0.462
T10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

43.434

100

0.448
T10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

42.424

100

0.437
T665 Streptococcus mutans UA159

45.455

95.652

0.435
T6127 Helicobacter pylori 26695

44.318

97.778

0.433
T28 Helicobacter pylori 26695

45.122

93.182

0.42
T10190 Pseudomonas sp. strain ANT_J3

42.857

94.792

0.406
T10088 Helicobacter pylori P12

41.111

96.774

0.398
T6290 Escherichia coli BW25113

40.449

96.739

0.391
T1038 Escherichia coli str. K-12 substr. MG1655

40.449

96.739

0.391
T10136 Pseudomonas savastanoi

42.529

90.625

0.385
T10070 Tetragenococcus halophilus NBRC 12172

39.13

97.872

0.383
T10108 Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes

53.488

70.492

0.377
T10181 Aggregatibacter actinomycetemcomitans D11S 1

39.286

95.455

0.375
T6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

36.17

97.917

0.354
T6289 Bifidobacterium longum subsp. longum JDM301

36.17

97.917

0.354
T10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

48.837

70.492

0.344
T6139 Lactobacillus rhamnosus Lc 705

48.837

70.492

0.344
T6144 Lactobacillus rhamnosus ATCC 8530

48.837

70.492

0.344
T6239 Lactobacillus rhamnosus ATCC 8530

48.837

70.492

0.344
T10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

34.483

91.579

0.316

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT6256 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

67.778

97.826

0.663
AT10048 Mycoplasma mycoides subsp. capri str. GM12

39.08

96.667

0.378
AT10190 Pseudomonas sp. strain ANT_J3

32.941

97.701

0.322
AT10136 Pseudomonas savastanoi

34.118

92.391

0.315
AT6290 Escherichia coli BW25113

29.885

100

0.302
AT1038 Escherichia coli str. K-12 substr. MG1655

29.885

100

0.302

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M4XBM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M4XB19

References

(1) R Álvarez et al. (2020) Evaluation of functionality of type II toxin-antitoxin systems of Clostridioides difficile R20291. Microbiological Research 239:126539. [PubMed:32622285] experimental


experimental experimental literature