Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-DinJ
Location 2065639..2066204 Replicon chromosome
Accession NZ_CP026591
Organism Clostridioides difficile strain CD-17-01474

Toxin (Protein)


Gene name relE Uniprot ID C7H6P5
Locus tag C3L34_RS10015 Protein ID WP_002596328.1
Coordinates 2065639..2065929 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C7H6P4
Locus tag C3L34_RS10020 Protein ID WP_005924829.1
Coordinates 2065926..2066204 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3L34_RS09980 2061235..2061696 + 462 WP_012816193.1 hypothetical protein -
C3L34_RS09990 2062141..2062554 + 414 WP_012816194.1 hypothetical protein -
C3L34_RS09995 2062656..2063108 + 453 WP_012816195.1 sigma-24 (feci) -
C3L34_RS10000 2063119..2063580 + 462 WP_009296689.1 sigma-70 family RNA polymerase sigma factor -
C3L34_RS10005 2063609..2063899 + 291 WP_024739841.1 DUF3847 domain-containing protein -
C3L34_RS10010 2064086..2065531 + 1446 WP_104732839.1 MobA/MobL family protein -
C3L34_RS10015 2065639..2065929 - 291 WP_002596328.1 type II toxin-antitoxin system YafQ family toxin Toxin
C3L34_RS10020 2065926..2066204 - 279 WP_005924829.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
C3L34_RS10025 2066331..2066729 + 399 WP_012816198.1 cysteine-rich VLP domain-containing protein -
C3L34_RS10030 2066843..2067577 + 735 WP_012816199.1 phage replisome organizer N-terminal domain-containing protein -
C3L34_RS10035 2067574..2068437 + 864 WP_104732840.1 ATP-binding protein -
C3L34_RS10040 2068481..2068675 + 195 WP_104732841.1 transposon-encoded TnpW family protein -
C3L34_RS10045 2068753..2069118 + 366 WP_104732842.1 hypothetical protein -
C3L34_RS10050 2069213..2069839 + 627 Protein_1839 TraM recognition domain-containing protein -
C3L34_RS10055 2069892..2070269 + 378 WP_009891894.1 TnpV protein -
C3L34_RS10060 2070283..2070564 + 282 WP_009891893.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2053693..2151173 97480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-90)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11112.87 Da        Isoelectric Point: 8.0630

>T95210 WP_002596328.1 NZ_CP026591:c2065929-2065639 [Clostridioides difficile]
MKETKYTVKYTTSFKKDYKRAIKRGLKIELLEQVVALLAMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Download         Length: 291 bp

>T95210 NZ_CP026591:c2065929-2065639 [Clostridioides difficile]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10247.66 Da        Isoelectric Point: 4.4692

>AT95210 WP_005924829.1 NZ_CP026591:c2066204-2065926 [Clostridioides difficile]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETVAAMLEAERIAKDPSVKGY
NDLDELFADLKR

Download         Length: 279 bp

>AT95210 NZ_CP026591:c2066204-2065926 [Clostridioides difficile]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M4XBM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M4XB19

References