105647

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-DinJ
Location 2069188..2069753 Replicon chromosome
Accession NZ_CP029423
Organism Clostridioides difficile R20291

Toxin (Protein)


Gene name relE Uniprot ID C7H6P5
Locus tag CDIF27147_RS09720 Protein ID WP_002596328.1
Coordinates 2069188..2069478 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C7H6P4
Locus tag CDIF27147_RS09725 Protein ID WP_005924829.1
Coordinates 2069475..2069753 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDIF27147_RS09690 2064784..2065245 + 462 WP_012816193.1 hypothetical protein -
CDIF27147_RS09695 2065690..2066103 + 414 WP_012816194.1 hypothetical protein -
CDIF27147_RS09700 2066205..2066657 + 453 WP_012816195.1 sigma-24 (feci) -
CDIF27147_RS09705 2066668..2067129 + 462 WP_009296689.1 sigma-70 family RNA polymerase sigma factor -
CDIF27147_RS09710 2067158..2067448 + 291 WP_024739841.1 DUF3847 domain-containing protein -
CDIF27147_RS09715 2067635..2069080 + 1446 WP_104732839.1 MobA/MobL family protein -
CDIF27147_RS09720 2069188..2069478 - 291 WP_002596328.1 type II toxin-antitoxin system YafQ family toxin Toxin
CDIF27147_RS09725 2069475..2069753 - 279 WP_005924829.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CDIF27147_RS09730 2069880..2070278 + 399 WP_012816198.1 cysteine-rich VLP domain-containing protein -
CDIF27147_RS09735 2070392..2071126 + 735 WP_012816199.1 phage replisome organizer N-terminal domain-containing protein -
CDIF27147_RS09740 2071123..2071986 + 864 WP_104732840.1 ATP-binding protein -
CDIF27147_RS09745 2072030..2072224 + 195 WP_104732841.1 transposon-encoded TnpW family protein -
CDIF27147_RS09750 2072302..2072667 + 366 WP_104732842.1 hypothetical protein -
CDIF27147_RS09755 2072762..2073388 + 627 Protein_1838 TraM recognition domain-containing protein -
CDIF27147_RS09760 2073441..2073818 + 378 WP_009891894.1 TnpV protein -
CDIF27147_RS09765 2073832..2074113 + 282 WP_009891893.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2057242..2154722 97480


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-90)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11112.87 Da        Isoelectric Point: 8.0630

>T105647 WP_002596328.1 NZ_CP029423:c2069478-2069188 [Clostridioides difficile R20291]
MKETKYTVKYTTSFKKDYKRAIKRGLKIELLEQVVALLAMGEPLPDKNRDHDLSGDWAGHRECHILPDWLLVYRIEDDVL
VLTLARTGTHSDLFGK

Download         Length: 291 bp

>T105647 NZ_CP029423:c2069478-2069188 [Clostridioides difficile R20291]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10247.66 Da        Isoelectric Point: 4.4692

>AT105647 WP_005924829.1 NZ_CP029423:c2069753-2069475 [Clostridioides difficile R20291]
MAGNTTNISIRMDADLKAQADALFTELGMNLTTAFNIFVRQSLREGGIPFEVRLEQPNKETVAAMLEAERIAKDPSVKGY
NDLDELFADLKR

Download         Length: 279 bp

>AT105647 NZ_CP029423:c2069753-2069475 [Clostridioides difficile R20291]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M4XBM1

Antitoxin

Source ID Structure
AlphaFold DB A0A1M4XB19

References