Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | fic-relB/- |
| Location | 892992..894044 | Replicon | chromosome |
| Accession | NZ_CP001668 | ||
| Organism | Mycoplasma mycoides subsp. capri str. GM12 | ||
Toxin (Protein)
| Gene name | Fic | Uniprot ID | A0A7Z7PMX0 |
| Locus tag | MMCAP2_RS03710 | Protein ID | WP_020862961.1 |
| Coordinates | 892992..893564 (+) | Length | 191 a.a. |
Antitoxin (Protein)
| Gene name | RelB | Uniprot ID | A0A7Z7PM07 |
| Locus tag | MMCAP2_RS03715 | Protein ID | WP_020862962.1 |
| Coordinates | 893772..894044 (+) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MMCAP2_RS03705 (MMCAP2_0751) | 889581..892622 | - | 3042 | WP_020862960.1 | viral A-type inclusion protein | - |
| MMCAP2_RS03710 (MMCAP2_0752) | 892992..893564 | + | 573 | WP_020862961.1 | Fic family protein | Toxin |
| MMCAP2_RS03715 (MMCAP2_0753) | 893772..894044 | + | 273 | WP_020862962.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| MMCAP2_RS03720 (MMCAP2_0754) | 894083..894988 | - | 906 | WP_038594791.1 | DNA adenine methylase | - |
| MMCAP2_RS03725 (MMCAP2_0755) | 894981..896069 | - | 1089 | WP_020862963.1 | DNA adenine methylase | - |
| MMCAP2_RS03730 (MMCAP2_0756) | 896186..898225 | + | 2040 | WP_020862964.1 | AlwI family type II restriction endonuclease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 191 a.a. Molecular weight: 22036.60 Da Isoelectric Point: 8.4325
>T10048 WP_020862961.1 NZ_CP001668:892992-893564 [Mycoplasma mycoides subsp. capri str. GM12]
MKANFLEEEFEINLSVKHLLELDKNLLNTFEIGTFKGLSQIHSYMFKDIFDFNGQIRNVNISKNNSMFCLARYLKQNLEI
IDNMKHDTFDQIIDKYVEMNICHPFREGNGRSMRILDLILKKQLNVVVNTNINKDEYLLAMINSLIDSTNLKLLIKNNLT
NKITDRNVYIKSIIKSYEYEGFKINIK
MKANFLEEEFEINLSVKHLLELDKNLLNTFEIGTFKGLSQIHSYMFKDIFDFNGQIRNVNISKNNSMFCLARYLKQNLEI
IDNMKHDTFDQIIDKYVEMNICHPFREGNGRSMRILDLILKKQLNVVVNTNINKDEYLLAMINSLIDSTNLKLLIKNNLT
NKITDRNVYIKSIIKSYEYEGFKINIK
Download Length: 573 bp
>T10048 NZ_CP001668:892992-893564 [Mycoplasma mycoides subsp. capri str. GM12]
ATGAAAGCTAATTTTTTAGAAGAAGAATTTGAGATTAATTTATCTGTTAAACACTTATTAGAGTTATGAGATAAAAATTT
ATTAAATACATTTGAAATAGGTACTTTTAAAGGTTTAAGTCAAATACATTCTTATATGTTTAAAGATATATTTGACTTTA
ATGGACAAATTAGAAATGTTAATATCTCTAAAAATAACTCTATGTTTTGTTTAGCAAGATATTTAAAACAAAACTTAGAA
ATTATTGATAATATGAAACATGATACATTTGATCAGATCATAGATAAATATGTTGAAATGAATATTTGTCATCCATTTAG
AGAAGGTAATGGCAGATCTATGAGAATTTGATTAGATTTAATATTAAAAAAACAACTAAATGTAGTTGTTAATTGAACTA
ATATAAATAAAGATGAGTACTTATTAGCAATGATTAATTCTTTAATTGATTCAACTAATCTTAAATTACTAATTAAAAAC
AACTTAACAAATAAGATTACTGATAGAAATGTATATATTAAAAGTATTATTAAATCTTATGAGTATGAAGGTTTTAAAAT
AAATATTAAATAA
ATGAAAGCTAATTTTTTAGAAGAAGAATTTGAGATTAATTTATCTGTTAAACACTTATTAGAGTTATGAGATAAAAATTT
ATTAAATACATTTGAAATAGGTACTTTTAAAGGTTTAAGTCAAATACATTCTTATATGTTTAAAGATATATTTGACTTTA
ATGGACAAATTAGAAATGTTAATATCTCTAAAAATAACTCTATGTTTTGTTTAGCAAGATATTTAAAACAAAACTTAGAA
ATTATTGATAATATGAAACATGATACATTTGATCAGATCATAGATAAATATGTTGAAATGAATATTTGTCATCCATTTAG
AGAAGGTAATGGCAGATCTATGAGAATTTGATTAGATTTAATATTAAAAAAACAACTAAATGTAGTTGTTAATTGAACTA
ATATAAATAAAGATGAGTACTTATTAGCAATGATTAATTCTTTAATTGATTCAACTAATCTTAAATTACTAATTAAAAAC
AACTTAACAAATAAGATTACTGATAGAAATGTATATATTAAAAGTATTATTAAATCTTATGAGTATGAAGGTTTTAAAAT
AAATATTAAATAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10451.07 Da Isoelectric Point: 8.4491
>AT10048 WP_020862962.1 NZ_CP001668:893772-894044 [Mycoplasma mycoides subsp. capri str. GM12]
MKKVNVNIKMDPEIKKQASLLFKEFGMTMSSAINLFVKTAVEQNDIPFEYNREITNKETLEAFKEGERLLADKNAKRYSS
FKEILDSLDK
MKKVNVNIKMDPEIKKQASLLFKEFGMTMSSAINLFVKTAVEQNDIPFEYNREITNKETLEAFKEGERLLADKNAKRYSS
FKEILDSLDK
Download Length: 273 bp
>AT10048 NZ_CP001668:893772-894044 [Mycoplasma mycoides subsp. capri str. GM12]
ATGAAAAAAGTAAATGTCAATATTAAAATGGATCCTGAAATTAAAAAACAGGCTAGTTTATTATTTAAAGAATTTGGTAT
GACCATGTCTTCTGCTATTAATCTATTTGTGAAAACTGCTGTTGAACAAAACGACATTCCATTTGAATATAATAGAGAGA
TAACTAACAAAGAAACTTTAGAAGCCTTTAAAGAAGGTGAACGTCTTTTAGCTGATAAAAACGCAAAACGTTACTCAAGT
TTTAAAGAAATATTAGATTCATTAGATAAATAA
ATGAAAAAAGTAAATGTCAATATTAAAATGGATCCTGAAATTAAAAAACAGGCTAGTTTATTATTTAAAGAATTTGGTAT
GACCATGTCTTCTGCTATTAATCTATTTGTGAAAACTGCTGTTGAACAAAACGACATTCCATTTGAATATAATAGAGAGA
TAACTAACAAAGAAACTTTAGAAGCCTTTAAAGAAGGTGAACGTCTTTTAGCTGATAAAAACGCAAAACGTTACTCAAGT
TTTAAAGAAATATTAGATTCATTAGATAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10095 | Clostridium difficile R20291 |
39.08 |
96.667 |
0.378 |
| AT6256 | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 |
38.095 |
93.333 |
0.356 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Z7PMX0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7Z7PM07 |
References
(1) Virginia Hill et al. (2021) Minimalistic mycoplasmas harbor different functional toxin-antitoxin systems. PLoS Genetics 17(10):e1009365. [PubMed:34673769]