6256

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/RelE(toxin)
Location 15155..15720 Replicon chromosome
Accession NC_011593
Organism Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

Toxin (Protein)


Gene name relE Uniprot ID B7GSH0
Locus tag BLON_RS00060 Protein ID WP_012576472.1
Coordinates 15155..15445 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B7GSH1
Locus tag BLON_RS00065 Protein ID WP_012576473.1
Coordinates 15442..15720 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BLON_RS00040 (Blon_0008) 10364..10930 + 567 WP_012576468.1 DUF3566 domain-containing protein -
BLON_RS00045 (Blon_0009) 11576..12997 + 1422 WP_012576469.1 ATP-binding protein -
BLON_RS00050 (Blon_0010) 13058..13282 - 225 WP_012576470.1 hypothetical protein -
BLON_RS00055 (Blon_0011) 13433..14779 - 1347 WP_012576471.1 NADP-specific glutamate dehydrogenase -
BLON_RS00060 (Blon_0012) 15155..15445 - 291 WP_012576472.1 type II toxin-antitoxin system YafQ family toxin Toxin
BLON_RS00065 (Blon_0013) 15442..15720 - 279 WP_012576473.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BLON_RS13135 (Blon_0014) 16200..16547 + 348 WP_162010634.1 hypothetical protein -
BLON_RS00075 16552..16947 - 396 WP_014484455.1 transposase -
BLON_RS00085 (Blon_0017) 17287..17931 + 645 WP_012576475.1 hypothetical protein -
BLON_RS00095 (Blon_0018) 18353..18544 - 192 WP_126386268.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(6-95)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-70)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10835.59 Da        Isoelectric Point: 10.3137

>T6256 WP_012576472.1 NC_011593:c15445-15155 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MSGIRYTVKTTSRFRKDFKLARRRGLDAGLFKQVVSILSEGGTLPDRYHDHALTGNMTGFRECYITPDLLLVYLIEKDVL
VLTLTRTGTHSGLFGK

Download         Length: 291 bp

>T6256 NC_011593:c15445-15155 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGAGCGGCATCCGGTACACGGTCAAGACCACGTCCAGATTCAGGAAGGACTTCAAACTCGCCAGACGACGCGGACTGGA
CGCCGGCCTTTTCAAACAGGTTGTATCCATTCTGTCCGAGGGCGGCACACTGCCCGACCGGTATCATGACCATGCGCTTA
CAGGCAATATGACCGGCTTCAGGGAGTGCTATATCACACCAGACCTGCTGTTGGTCTATCTCATAGAGAAGGACGTGCTG
GTGCTCACTCTGACCCGAACGGGCACCCATAGTGGCCTTTTCGGAAAATAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10202.63 Da        Isoelectric Point: 6.3216

>AT6256 WP_012576473.1 NC_011593:c15720-15442 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
MSGTTTTVNVRMNAELKKQAEELFGELGMNLTTAVNIFIRQSLRQGGIPFNVSLDEPNRETVSAMLEAKRIARDGNVKGY
RDLNALFKDLDA

Download         Length: 279 bp

>AT6256 NC_011593:c15720-15442 [Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM]
ATGAGCGGAACGACAACCACCGTCAACGTCCGAATGAACGCTGAGCTGAAAAAACAAGCCGAGGAACTGTTCGGGGAGCT
GGGCATGAACCTGACCACCGCAGTCAACATATTCATCCGCCAATCCCTGCGACAGGGCGGCATACCGTTCAACGTCTCGT
TGGATGAGCCGAACAGGGAGACCGTCTCCGCCATGTTGGAGGCGAAGCGAATCGCAAGGGACGGGAACGTGAAGGGCTAC
CGGGACCTGAACGCCTTGTTCAAGGACTTGGATGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10095 Clostridium difficile R20291

59.375

100

0.594
T6286 Campylobacter jejuni subsp. jejuni IA3902

44.444

98.901

0.44
T6127 Helicobacter pylori 26695

44.318

97.778

0.433
T10088 Helicobacter pylori P12

43.333

96.774

0.419
T166 Vibrio cholerae O1 biovar El Tor str. N16961

41.111

100

0.416
T28 Helicobacter pylori 26695

43.21

92.045

0.398
T10136 Pseudomonas savastanoi

38.542

100

0.385
T6290 Escherichia coli BW25113

36.17

100

0.37
T1038 Escherichia coli str. K-12 substr. MG1655

36.17

100

0.37
T10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

35.354

100

0.365
T10108 Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes

52.381

68.852

0.361
T10181 Aggregatibacter actinomycetemcomitans D11S 1

36.905

95.455

0.352
T6144 Lactobacillus rhamnosus ATCC 8530

50

68.852

0.344
T6239 Lactobacillus rhamnosus ATCC 8530

50

68.852

0.344
T10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

50

68.852

0.344
T6139 Lactobacillus rhamnosus Lc 705

50

68.852

0.344
T10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

33.333

100

0.344
T665 Streptococcus mutans UA159

35.227

95.652

0.337
T10070 Tetragenococcus halophilus NBRC 12172

31.25

100

0.319
T10109 Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

35.294

89.474

0.316
T6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

32.258

96.875

0.312
T6289 Bifidobacterium longum subsp. longum JDM301

32.258

96.875

0.312
T10190 Pseudomonas sp. strain ANT_J3

32.222

93.75

0.302

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10095 Clostridium difficile R20291

67.778

97.826

0.663
AT10048 Mycoplasma mycoides subsp. capri str. GM12

38.095

93.333

0.356
AT10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

31.034

97.753

0.303
AT10111 Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

31.034

97.753

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB B7GSH0

Antitoxin

Source ID Structure
AlphaFold DB A0A0A8MHE5

References

(1) Olga Averina et al. (2015) Functional analysis of the type II toxin-antitoxin systems of the MazEF and RelBE families in Bifidobacterium longum subsp. infantis ATCC 15697. Anaerobe 35(Pt B):59-67. [PubMed:26210151] experimental


experimental experimental literature