10136

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) relBE/-
Location 23533..24109 Replicon plasmid pPsv48A
Accession NC_019265
Organism Pseudomonas savastanoi

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag PSA3335_RS00100 Protein ID WP_015060653.1
Coordinates 23533..23853 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID H1ZWU0
Locus tag PSA3335_RS00105 Protein ID WP_015060654.1
Coordinates 23780..24109 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSA3335_RS00070 (PSA3335_A0014) 19110..19796 - 687 WP_244657733.1 hypothetical protein -
PSA3335_RS00075 (PSA3335_A0015) 20376..20657 - 282 WP_004666803.1 hypothetical protein -
PSA3335_RS00080 (PSA3335_A0016) 20657..21340 - 684 WP_002556051.1 AAA family ATPase -
PSA3335_RS00085 (PSA3335_A0017) 21678..21935 + 258 Protein_16 TraR/DksA family transcriptional regulator -
PSA3335_RS00090 (PSA3335_A0065) 21973..22194 + 222 WP_015060651.1 hypothetical protein -
PSA3335_RS00095 (PSA3335_A0018) 22526..23146 - 621 WP_005747119.1 recombinase family protein -
PSA3335_RS00100 (PSA3335_A0019) 23533..23853 - 321 WP_015060653.1 type II toxin-antitoxin system YafQ family toxin Toxin
PSA3335_RS00105 (PSA3335_A0020) 23780..24109 - 330 WP_015060654.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
PSA3335_RS00110 (PSA3335_A0021) 24464..25693 - 1230 WP_082301212.1 IS91 family transposase -
PSA3335_RS00115 25743..25955 - 213 WP_244657730.1 hypothetical protein -
PSA3335_RS00120 (PSA3335_A0023) 25997..26899 + 903 WP_168550530.1 hypothetical protein -
PSA3335_RS00125 27083..27316 + 234 WP_053481311.1 hypothetical protein -
PSA3335_RS00130 (PSA3335_A0024) 28241..28945 - 705 WP_015060656.1 isopentenyl transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..80123 80123
- inside IScluster/Tn - - 24464..39528 15064


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(22-105)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(9-70)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12336.19 Da        Isoelectric Point: 9.8612

>T10136 WP_015060653.1 NC_019265:c23853-23533 [Pseudomonas savastanoi pv. savastanoi]
MAKKPARPANLKRAPLPKRTAYTPEFKKSWKRHNDAGRQPMTEARDVMRMLWEGDTLPAQYLDHELQGEWAGNRECHIRG
DFLLVYQVTKTDVIFVDIGTHAELFK

Download         Length: 321 bp

>T10136 NC_019265:c23853-23533 [Pseudomonas savastanoi pv. savastanoi]
ATGGCAAAGAAGCCCGCAAGACCGGCAAATCTAAAGCGCGCCCCGTTGCCAAAGAGAACGGCGTACACGCCTGAATTTAA
GAAATCATGGAAGCGGCACAATGATGCCGGCCGGCAGCCTATGACCGAAGCCAGGGATGTGATGAGAATGCTTTGGGAGG
GTGATACGTTGCCGGCTCAGTACCTAGATCACGAATTGCAAGGCGAATGGGCGGGTAACCGAGAATGCCATATCCGAGGA
GATTTCTTACTGGTCTACCAGGTTACGAAAACAGATGTGATTTTTGTTGATATAGGCACTCATGCAGAGCTGTTCAAATG
A

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12129.91 Da        Isoelectric Point: 9.3061

>AT10136 WP_015060654.1 NC_019265:c24109-23780 [Pseudomonas savastanoi pv. savastanoi]
MTALLKNTDVRCRIEEDLKERATAVLSACGLSLSDAMRLFLRQVVETRGLPFEVKAPSEKTARALAQAREIRSRFDSIDE
LFEDLDGKEARKTGKSKARPVAKENGVHA

Download         Length: 330 bp

>AT10136 NC_019265:c24109-23780 [Pseudomonas savastanoi pv. savastanoi]
ATGACAGCACTACTGAAAAATACCGACGTGCGTTGCCGCATCGAAGAAGATTTGAAAGAGCGAGCTACTGCAGTGCTGAG
CGCTTGCGGCCTGAGCCTGAGCGATGCGATGCGTCTCTTTCTTCGTCAAGTTGTGGAAACCAGGGGGCTCCCTTTTGAAG
TGAAGGCGCCTTCCGAGAAAACGGCTCGAGCTTTGGCTCAAGCCCGTGAGATCCGCAGTCGGTTCGACTCGATTGACGAA
TTGTTCGAGGATCTAGATGGCAAAGAAGCCCGCAAGACCGGCAAATCTAAAGCGCGCCCCGTTGCCAAAGAGAACGGCGT
ACACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT10190 Pseudomonas sp. strain ANT_J3

38.272

93.103

0.356
AT1038 Escherichia coli str. K-12 substr. MG1655

35.366

95.349

0.337
AT6290 Escherichia coli BW25113

35.366

95.349

0.337
AT6239 Lactobacillus rhamnosus ATCC 8530

33.333

96.667

0.322
AT6139 Lactobacillus rhamnosus Lc 705

33.333

96.667

0.322
AT10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

33.333

96.667

0.322
AT6144 Lactobacillus rhamnosus ATCC 8530

33.333

96.667

0.322
AT10095 Clostridium difficile R20291

34.118

92.391

0.315
AT6257 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

33.735

93.258

0.315
AT166 Vibrio cholerae O1 biovar El Tor str. N16961

35

86.957

0.304
AT6289 Bifidobacterium longum subsp. longum JDM301

32.927

92.135

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB H1ZWU0

References

(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866] experimental


experimental experimental literature