10111

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/-
Location 1..565 Replicon chromosome
Accession MK544944
Organism Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag Locus_1 Protein ID QIP66200.1
Coordinates 263..565 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID -
Locus tag Locus_0 Protein ID QIP66199.1
Coordinates 1..270 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_0 1..270 + 270 QIP66199.1 DinJ Antitoxin
Locus_1 263..565 + 303 QIP66200.1 YafQ Toxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T10111 QIP66200.1 MK544944:263-565 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T10111 MK544944:263-565 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT10111 QIP66199.1 MK544944:1-270 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT10111 MK544944:1-270 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT10112 Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes

98.876

100

0.989
AT665 Streptococcus mutans UA159

35.714

94.382

0.337
AT6139 Lactobacillus rhamnosus Lc 705

32.927

92.135

0.303
AT10110 Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes

32.927

92.135

0.303
AT6239 Lactobacillus rhamnosus ATCC 8530

32.927

92.135

0.303
AT6144 Lactobacillus rhamnosus ATCC 8530

32.927

92.135

0.303
AT6256 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222

31.034

97.753

0.303

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Alessia Levante et al. (2019) Expression of DinJ-YafQ System of Lactobacillus casei Group Strains in Response to Food Processing Stresses. Microorganisms 7(10):438. [PubMed:31614503] experimental

(2) Alberto Ferrari et al. (2019) Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest. Scientific Reports 9(1):7645. [PubMed:31114007] experimental


experimental experimental literature