Detailed information of TA system TA006127

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/-
• Location: 945691..946264
• Replicon: chromosome
• Accession: NC_000915
• Organism: Helicobacter pylori 26695

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O25552
• Locus tag: HP_RS04350
• Protein ID: WP_000955518.1
• Coordinates: 945691..945963 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O25553
• Locus tag: HP_RS04355
• Protein ID: WP_001134157.1
• Coordinates: 945977..946264 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP_RS04330 (HP0888)	942347..943114	-	768	WP_000242349.1	ABC transporter ATP-binding protein	-
HP_RS04335 (HP0889)	943114..944094	-	981	WP_000921458.1	iron ABC transporter permease	-
HP_RS04340 (HP0890)	944087..944935	-	849	WP_000504124.1	SDR family oxidoreductase	-
HP_RS04345 (HP0891)	945075..945599	+	525	WP_001135603.1	acyl-CoA thioesterase	-
HP_RS04350 (HP0892)	945691..945963	-	273	WP_000955518.1	type II toxin-antitoxin system mRNA interferase toxin, HP0892 family	Toxin
HP_RS04355 (HP0893)	945977..946264	-	288	WP_001134157.1	type II toxin-antitoxin system antitoxin	Antitoxin
HP_RS04360 (HP0894)	946345..946611	-	267	WP_000916169.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
HP_RS04365 (HP0895)	946592..946969	-	378	WP_001134075.1	hypothetical protein	-
HP_RS04370 (HP0896)	947580..949706	-	2127	WP_000753173.1	Hop family adhesin BabB	-
HP_RS04385	950510..950773	-	264	WP_015056067.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10412.36 Da
• Isoelectric Point: 9.9607

>T6127 WP_000955518.1 NC_000915:c945963-945691 [Helicobacter pylori 26695]
MLTIETSKKFDKDLKILVKNGFDLKLLYKVVGNLATEQPLAPKYKDHPLKGGLKDFRECHLKPDLLLVYQIKKQENTLFL
VRLGSHSELF

Nucleotide

• Length: 273 bp

>T6127 NC_000915:c945963-945691 [Helicobacter pylori 26695]
ATGCTGACGATTGAAACCAGTAAAAAATTTGATAAGGATCTTAAAATTCTTGTTAAAAACGGGTTTGATTTAAAGCTTTT
GTATAAAGTGGTTGGAAATTTAGCCACAGAGCAACCCCTAGCTCCCAAATACAAAGACCACCCACTCAAAGGCGGTTTAA
AAGATTTTAGGGAATGCCACTTAAAACCGGATTTATTGCTTGTCTATCAAATTAAAAAACAAGAAAACACCCTCTTTTTA
GTAAGGTTAGGCAGTCATAGCGAGCTGTTTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11356.14 Da
• Isoelectric Point: 10.4454

>AT6127 WP_001134157.1 NC_000915:c946264-945977 [Helicobacter pylori 26695]
MPNTTNKDYTKYSQRQLFSFLNSIKTKQKRALEKLKEIQAQKQRIKKALQFKALNLTENGYTIEEEREILARAKDTKNRL
CFKSIEDFKKHCENL

Nucleotide

• Length: 288 bp

>AT6127 NC_000915:c946264-945977 [Helicobacter pylori 26695]
ATGCCAAACACCACCAACAAAGACTACACAAAATACAGCCAAAGACAGCTTTTTAGTTTTTTAAATTCTATCAAGACCAA
GCAAAAAAGAGCGTTAGAAAAATTGAAAGAAATCCAAGCTCAAAAACAAAGGATTAAAAAAGCACTCCAATTTAAAGCGC
TAAACTTAACCGAAAATGGATACACCATAGAAGAAGAGCGAGAAATCTTAGCAAGGGCTAAGGACACTAAGAACCGCCTT
TGTTTTAAAAGCATAGAGGATTTTAAGAAGCATTGTGAAAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T28	Helicobacter pylori 26695	52.222	100	0.534
T10088	Helicobacter pylori P12	50.549	100	0.511
T6286	Campylobacter jejuni subsp. jejuni IA3902	47.727	97.778	0.467
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	44.318	97.778	0.433
T10095	Clostridium difficile R20291	44.318	97.778	0.433
T166	Vibrio cholerae O1 biovar El Tor str. N16961	41.758	100	0.427
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	36.735	100	0.4
T10181	Aggregatibacter actinomycetemcomitans D11S 1	40.23	98.864	0.398
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	35.714	100	0.389
T10190	Pseudomonas sp. strain ANT_J3	38.636	97.778	0.378
T665	Streptococcus mutans UA159	36.667	100	0.367
T1038	Escherichia coli str. K-12 substr. MG1655	37.5	97.778	0.367
T6290	Escherichia coli BW25113	37.5	97.778	0.367
T10070	Tetragenococcus halophilus NBRC 12172	34.118	94.444	0.322
T10109	Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes	31.461	98.889	0.311
T6257	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	28.866	100	0.311
T6289	Bifidobacterium longum subsp. longum JDM301	28.866	100	0.311

Multiple sequence alignment

Structures

Toxin

• PDB: 2OTR
• PDB: 4NRN

Antitoxin

• AlphaFold DB: A0A1Y3E1W9

References

(1) Kyung-Doo Han et al. (2013) Identification of chromosomal HP0892-HP0893 toxin-antitoxin proteins in Helicobacter pylori and structural elucidation of their protein-protein interaction. The Journal of Biological Chemistry 288(8):6004-13. [PubMed:23297406]

(2) Hookang Im et al. (2014) Crystal structure of toxin HP0892 from Helicobacter pylori with two Zn(II) at 1.8 Å resolution. Protein Science : A Publication of The Protein Society 23(6):819-32. [PubMed:24677509]


 experimental literature
 protein structure literature