Detailed information of TA system TA010109

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/-
• Location: 1..544
• Replicon: chromosome
• Accession: MK544946
• Organism: Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: Locus_1
• Protein ID: QIP66204.1
• Coordinates: 257..544 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: Locus_0
• Protein ID: QIP66203.1
• Coordinates: 1..273 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	1..273	+	273	QIP66203.1	DinJ	Antitoxin
Locus_1	257..544	+	288	QIP66204.1	YafQ	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-67)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10869.72 Da
• Isoelectric Point: 10.6953

>T10109 QIP66204.1 MK544946:257-544 [Lacticaseibacillus rhamnosus]
MMLTINRTRTFKRQFKHLLRQGKDMTKLATAIDTLQRQDRVKLASLHDHALKGAHSGERALHVAPDWLLVYKVDAEALIL
MLLATGTHRDTLNIE

Nucleotide

• Length: 288 bp

>T10109 MK544946:257-544 [Lacticaseibacillus rhamnosus]
ATGATGTTGACGATTAATCGCACGCGCACTTTCAAACGACAATTTAAACATCTTCTTAGGCAAGGTAAAGATATGACGAA
ACTTGCAACTGCAATTGATACTTTGCAGCGTCAAGATCGTGTGAAATTAGCTTCTTTACACGACCATGCTTTAAAGGGTG
CTCACAGCGGCGAACGAGCATTGCATGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTA
ATGCTTCTTGCAACAGGCACACACCGCGATACATTGAACATTGAGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10073.49 Da
• Isoelectric Point: 5.1154

>AT10109 QIP66203.1 MK544946:1-273 [Lacticaseibacillus rhamnosus]
METKSRISVRIDTKTKERALHVLNSMGLDMSSAINMYLKRIGDTGELPFTPEMSFADQLQAAEADVKAGRTKSFKTVDAL
MKDLYNDVDD

Nucleotide

• Length: 273 bp

>AT10109 MK544946:1-273 [Lacticaseibacillus rhamnosus]
ATGGAAACAAAATCCCGTATCAGCGTGCGAATTGATACCAAAACCAAAGAACGGGCTCTCCATGTCCTCAATAGCATGGG
ACTAGATATGTCCTCAGCTATTAACATGTATTTGAAACGCATTGGTGACACTGGTGAGTTGCCATTTACACCTGAAATGT
CGTTCGCCGATCAGCTTCAAGCTGCAGAAGCTGATGTTAAAGCGGGACGAACTAAGAGCTTCAAGACCGTTGATGCCCTG
ATGAAGGATTTATACAATGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6239	Lactobacillus rhamnosus ATCC 8530	93.443	100	0.934
T6139	Lactobacillus rhamnosus Lc 705	93.443	100	0.934
T10110	Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes	93.443	100	0.934
T6144	Lactobacillus rhamnosus ATCC 8530	93.443	100	0.934
T10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	91.803	100	0.918
T665	Streptococcus mutans UA159	41.935	100	0.424
T10111	Lacticaseibacillus paracasei strain 4366 DinJ and YafQ genes	39.394	100	0.411
T10112	Lacticaseibacillus paracasei strain 2333 DinJ and YafQ genes	38.384	100	0.4
T28	Helicobacter pylori 26695	37.079	100	0.375
T10190	Pseudomonas sp. strain ANT_J3	35.955	93.684	0.337
T6286	Campylobacter jejuni subsp. jejuni IA3902	34.118	93.407	0.319
T10095	Clostridium difficile R20291	34.483	91.579	0.316
T6256	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	35.294	89.474	0.316
T1038	Escherichia coli str. K-12 substr. MG1655	34.118	92.391	0.315
T6290	Escherichia coli BW25113	34.118	92.391	0.315
T6127	Helicobacter pylori 26695	31.461	98.889	0.311
T166	Vibrio cholerae O1 biovar El Tor str. N16961	30	100	0.303
T10088	Helicobacter pylori P12	31.111	96.774	0.301

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT6139	Lactobacillus rhamnosus Lc 705	90	100	0.9
AT10110	Lacticaseibacillus rhamnosus strain 1019 DinJ and YafQ genes	90	100	0.9
AT6144	Lactobacillus rhamnosus ATCC 8530	90	100	0.9
AT6239	Lactobacillus rhamnosus ATCC 8530	90	100	0.9
AT10108	Lacticaseibacillus rhamnosus strain 1473 DinJ and YafQ genes	75.556	100	0.756

Multiple sequence alignment

References

(1) Alessia Levante et al. (2019) Expression of DinJ-YafQ System of Lactobacillus casei Group Strains in Response to Food Processing Stresses. Microorganisms 7(10):438. [PubMed:31614503]

(2) Alberto Ferrari et al. (2019) Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest. Scientific Reports 9(1):7645. [PubMed:31114007]


 experimental literature