Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/- |
| Location | 475253..475841 | Replicon | chromosome |
| Accession | NC_011498 | ||
| Organism | Helicobacter pylori P12 | ||
Toxin (Protein)
| Gene name | TfiT | Uniprot ID | M3P300 |
| Locus tag | HPP12_RS02330 | Protein ID | WP_000921941.1 |
| Coordinates | 475560..475841 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | TfiA | Uniprot ID | B6JL30 |
| Locus tag | HPP12_RS02325 | Protein ID | WP_000073118.1 |
| Coordinates | 475253..475567 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HPP12_RS02305 (HPP12_0448) | 470785..471441 | + | 657 | WP_000587389.1 | ParA family protein | - |
| HPP12_RS02310 (HPP12_0449) | 471523..471807 | + | 285 | WP_000394639.1 | hypothetical protein | - |
| HPP12_RS02315 (HPP12_0450) | 471839..473017 | + | 1179 | WP_001044521.1 | hypothetical protein | - |
| HPP12_RS02320 (HPP12_0451) | 473042..474955 | - | 1914 | WP_001162729.1 | relaxase/mobilization nuclease domain-containing protein | - |
| HPP12_RS02325 (HPP12_0452) | 475253..475567 | + | 315 | WP_000073118.1 | hypothetical protein | Antitoxin |
| HPP12_RS02330 (HPP12_0453) | 475560..475841 | + | 282 | WP_000921941.1 | YafQ family addiction module toxin | Toxin |
| HPP12_RS02335 (HPP12_0454) | 476105..477832 | - | 1728 | WP_001208212.1 | type IV secretory system conjugative DNA transfer family protein | - |
| HPP12_RS02340 (HPP12_0455) | 477833..478391 | - | 559 | Protein_452 | hypothetical protein | - |
| HPP12_RS02345 (HPP12_0456) | 478392..478682 | - | 291 | WP_000159813.1 | hypothetical protein | - |
| HPP12_RS02350 (HPP12_0457) | 478679..479134 | - | 456 | WP_000795435.1 | hypothetical protein | - |
| HPP12_RS02355 (HPP12_0458) | 479131..480072 | - | 942 | WP_000625138.1 | ATPase, T2SS/T4P/T4SS family | - |
| HPP12_RS02360 (HPP12_0459) | 480072..480371 | - | 300 | WP_000660141.1 | hypothetical protein | - |
| HPP12_RS02365 (HPP12_0460) | 480368..480631 | - | 264 | WP_000564645.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | - | 449944..533667 | 83723 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 10891.80 Da Isoelectric Point: 9.5726
>T10088 WP_000921941.1 NC_011498:475560-475841 [Helicobacter pylori P12]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK
Download Length: 282 bp
>T10088 NC_011498:475560-475841 [Helicobacter pylori P12]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAGAGCGA
TGTTACAAGCGTTGTTGATTGCCTAAAAGAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGCGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCTGGGCGTATTACAGAGAGCGA
TGTTACAAGCGTTGTTGATTGCCTAAAAGAACAAAAACCACTCCAACAAAAATATTGCGACCATGCTTTGAGCGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12199.67 Da Isoelectric Point: 5.7670
>AT10088 WP_000073118.1 NC_011498:475253-475567 [Helicobacter pylori P12]
MSNVITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKRSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA
MSNVITDYSQYNEKQLHNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKRSQEKEPTPSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA
Download Length: 315 bp
>AT10088 NC_011498:475253-475567 [Helicobacter pylori P12]
ATGTCAAATGTTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACGTTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA
ATGTCAAATGTTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAAG
TTTTAGCCCAAAAACGTTCTCAAGAAAAAGAACCCACACCAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCATAATGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6127 | Helicobacter pylori 26695 |
50.549 |
100 |
0.511 |
| T28 | Helicobacter pylori 26695 |
47.253 |
100 |
0.489 |
| T6256 | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 |
43.333 |
96.774 |
0.419 |
| T6286 | Campylobacter jejuni subsp. jejuni IA3902 |
40.659 |
100 |
0.407 |
| T166 | Vibrio cholerae O1 biovar El Tor str. N16961 |
39.13 |
100 |
0.404 |
| T10095 | Clostridium difficile R20291 |
41.111 |
96.774 |
0.398 |
| T10070 | Tetragenococcus halophilus NBRC 12172 |
35.484 |
100 |
0.355 |
| T10181 | Aggregatibacter actinomycetemcomitans D11S 1 |
44.928 |
78.409 |
0.352 |
| T10136 | Pseudomonas savastanoi |
34.524 |
90.323 |
0.312 |
| T10190 | Pseudomonas sp. strain ANT_J3 |
32.184 |
93.548 |
0.301 |
| T10109 | Lacticaseibacillus rhamnosus strain 2360 DinJ and YafQ genes |
31.111 |
96.774 |
0.301 |
| T665 | Streptococcus mutans UA159 |
31.818 |
94.624 |
0.301 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Kwadwo Boampong et al. (2020) Rapid growth inhibitory activity of a YafQ-family endonuclease toxin of the Helicobacter pylori tfs4 integrative and conjugative element. Scientific Reports 10(1):18171. [PubMed:33097748]