Detailed information of TA system
Experimentally validatedOverview
TA module
Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/RHH-RelE |
Location | 245961..246502 | Replicon | chromosome |
Accession | NC_000913 | ||
Organism | Escherichia coli str. K-12 substr. MG1655 |
Toxin (Protein)
Gene name | yafQ | Uniprot ID | Q47149 |
Locus tag | b0225 | Protein ID | NP_414760.1 |
Coordinates | 245961..246239 (-) | Length | 93 a.a. |
Antitoxin (Protein)
Gene name | dinJ | Uniprot ID | Q47150 |
Locus tag | b0226 | Protein ID | NP_414761.1 |
Coordinates | 246242..246502 (-) | Length | 87 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
b0222 | 243543..244121 | + | 579 | NP_414757.1 | D-sedoheptulose 7-phosphate isomerase | - |
b0223 | 244327..245094 | + | 768 | NP_414758.1 | putative glutamine amidotransferase YafJ | - |
b0224 | 245065..245805 | - | 741 | NP_414759.1 | peptidoglycan meso-diaminopimelic acid protein amidase A | - |
b0225 | 245961..246239 | - | 279 | NP_414760.1 | ribosome-dependent mRNA interferase toxin YafQ | Toxin |
b0226 | 246242..246502 | - | 261 | NP_414761.1 | antitoxin/DNA-binding transcriptional repressor DinJ | Antitoxin |
b0227 | 246712..247461 | + | 750 | NP_414762.1 | NlpC/P60 family protein YafL | - |
b0228 | 247637..248134 | + | 498 | NP_414763.1 | REP-associated tyrosine transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10846.62 Da Isoelectric Point: 9.9941
>T1038 NP_414760.1 NC_000913:c246239-245961 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG
Download Length: 279 bp
>T1038 NC_000913:c246239-245961 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9405.80 Da Isoelectric Point: 5.0758
>AT1038 NP_414761.1 NC_000913:c246502-246242 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI
Download Length: 261 bp
>AT1038 NC_000913:c246502-246242 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Multiple sequence alignment
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|---|---|---|---|
AT6290 | Escherichia coli BW25113 |
100 |
100 |
1 |
AT10190 | Pseudomonas sp. strain ANT_J3 |
38.961 |
89.535 |
0.349 |
AT6097 | Sinorhizobium meliloti 1021 |
56.863 |
59.302 |
0.337 |
AT10136 | Pseudomonas savastanoi |
35.366 |
95.349 |
0.337 |
AT10095 | Clostridium difficile R20291 |
29.885 |
100 |
0.302 |
Multiple sequence alignment
References
(1) Ajchareeya Ruangprasert et al. (2014) Mechanisms of toxin inhibition and transcriptional repression by Escherichia coli DinJ-YafQ. The Journal of Biological Chemistry 289(30):20559-69. [PubMed:24898247]
(2) Meredith H Prysak et al. (2009) Bacterial toxin YafQ is an endoribonuclease that associates with the ribosome and blocks translation elongation through sequence-specific and frame-dependent mRNA cleavage. Molecular Microbiology 71(5):1071-87. [PubMed:19210620]
(3) Rūta Motiejūnaite et al. (2007) Escherichia coli dinJ-yafQ genes act as a toxin-antitoxin module. FEMS Microbiology Letters 268(1):112-9. [PubMed:17263853]
(4) Ilana Kolodkin-Gal et al. (2009) A differential effect of E. coli toxin-antitoxin systems on cell death in liquid media and biofilm formation. PloS One 4(8):e6785. [PubMed:19707553]
experimental literature