Detailed information of TA system TA006097

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH-RelE
• Location: 258225..258823
• Replicon: plasmid pSymA
• Accession: NC_003037
• Organism: Sinorhizobium meliloti 1021

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: SM_RS26010
• Protein ID: WP_010967243.1
• Coordinates: 258530..258823 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: SM_RS26005
• Protein ID: WP_010967242.1
• Coordinates: 258225..258533 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM_RS25990 (SMa0467)	253863..254879	+	1017	WP_010967239.1	ABC transporter permease	-
SM_RS25995 (SMa0469)	254876..255718	+	843	WP_015445407.1	ABC transporter permease	-
SM_RS26000 (SMa0470)	255715..257562	+	1848	WP_015445408.1	ABC transporter ATP-binding protein	-
SM_RS26005 (SMa0471)	258225..258533	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SM_RS26010 (SMa0473)	258530..258823	+	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
SM_RS26015 (SMa0475)	259012..260349	+	1338	Protein_258	ISNCY-like element ISRm17 family transposase	-
SM_RS26020 (SMa0478)	260764..261963	-	1200	WP_041169958.1	NAD-dependent formate dehydrogenase	-
SM_RS26025	262378..263128	+	751	Protein_260	RepB plasmid partition	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_020527 Sinorhizobium meliloti 2011 Conjugative plasmid TA029033, TA029034, TA029035, TA029036 1..1352561 0 View NZ_CP021798 Sinorhizobium meliloti strain USDA1106 Conjugative plasmid TA077440, TA077441, TA077442, TA077443 1..1363628 0	inside	Conjugative plasmid	-	htpB	1..1354226	1354226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T6097 WP_010967243.1 NC_003037:258530-258823 [Sinorhizobium meliloti 1021]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T6097 NC_003037:258530-258823 [Sinorhizobium meliloti 1021]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT6097 WP_010967242.1 NC_003037:258225-258533 [Sinorhizobium meliloti 1021]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT6097 NC_003037:258225-258533 [Sinorhizobium meliloti 1021]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10119	Escherichia coli	43.011	100	0.435
T6134	Paracoccus aminophilus	43.158	97.938	0.423
T10139	Pseudomonas savastanoi	40.23	95.604	0.385
T179	Vibrio cholerae O1 biovar El Tor str. N16961	38.462	98.913	0.38
T168	Vibrio cholerae O1 biovar El Tor str. N16961	38.462	98.913	0.38
T10143	Pseudomonas savastanoi	38.043	100	0.38
T705	Vibrio parahaemolyticus RIMD 2210633	36.264	98.913	0.359
T1006	Escherichia coli O157:H7 str. Sakai	33.696	96.842	0.326
T178	Vibrio cholerae O1 biovar El Tor str. N16961	34.831	91.753	0.32
T180	Pseudomonas aeruginosa PAO1	33.721	92.473	0.312
T229	Caulobacter crescentus CB15	34.483	89.691	0.309

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT1038	Escherichia coli str. K-12 substr. MG1655	56.863	59.302	0.337
AT6290	Escherichia coli BW25113	56.863	59.302	0.337
AT1041	Escherichia coli str. K-12 substr. MG1655	34.667	94.937	0.329

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1

References

(1) Branislava Milunovic et al. (2014) Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti. Journal of Bacteriology 196(4):811-24. [PubMed:24317400]


 experimental literature