77442

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 830441..831021 Replicon plasmid psymA
Accession NZ_CP021798
Organism Sinorhizobium meliloti strain USDA1106

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag CDO30_RS22410 Protein ID WP_010968151.1
Coordinates 830441..830824 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag CDO30_RS22415 Protein ID WP_013851145.1
Coordinates 830821..831021 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDO30_RS22395 826026..827030 + 1005 WP_010968154.1 hypothetical protein -
CDO30_RS22400 827320..828984 - 1665 WP_015445521.1 hypothetical protein -
CDO30_RS22405 829210..829989 + 780 WP_010968152.1 hypothetical protein -
CDO30_RS22410 830441..830824 - 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
CDO30_RS22415 830821..831021 - 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CDO30_RS22420 831536..832063 + 528 WP_080590796.1 TIGR02444 family protein -
CDO30_RS22425 832254..833027 + 774 WP_014528246.1 UTRA domain-containing protein -
CDO30_RS22430 833119..834495 - 1377 WP_010968148.1 NAD(P)/FAD-dependent oxidoreductase -
CDO30_RS22435 834492..834806 - 315 WP_015445520.1 (2Fe-2S)-binding protein -
CDO30_RS22440 834796..835902 - 1107 WP_010968147.1 FAD-binding oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - htpB 1..1363628 1363628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T77442 WP_010968151.1 NZ_CP021798:c830824-830441 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T77442 NZ_CP021798:c830824-830441 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT77442 WP_013851145.1 NZ_CP021798:c831021-830821 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT77442 NZ_CP021798:c831021-830821 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References