20642

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1249119..1249699 Replicon plasmid pSymA
Accession NC_003037
Organism Sinorhizobium meliloti 1021

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag SM_RS30750 Protein ID WP_010968151.1
Coordinates 1249316..1249699 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag SM_RS30745 Protein ID WP_013851145.1
Coordinates 1249119..1249319 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM_RS30720 (SMa2223) 1244238..1245344 + 1107 WP_010968147.1 FAD-binding oxidoreductase -
SM_RS30725 1245334..1245648 + 315 WP_015445520.1 (2Fe-2S)-binding protein -
SM_RS30730 (SMa2225) 1245645..1247021 + 1377 WP_010968148.1 NAD(P)/FAD-dependent oxidoreductase -
SM_RS30735 (SMa2227) 1247113..1247886 - 774 WP_010968149.1 UTRA domain-containing protein -
SM_RS30740 (SMa2229) 1248077..1248604 - 528 WP_080590796.1 TIGR02444 family protein -
SM_RS30745 1249119..1249319 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SM_RS30750 (SMa2231) 1249316..1249699 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
SM_RS30755 (SMa2233) 1250151..1250930 - 780 WP_010968152.1 hypothetical protein -
SM_RS30760 (SMa2235) 1251156..1252820 + 1665 WP_015445521.1 hypothetical protein -
SM_RS30765 (SMa2237) 1253110..1254114 - 1005 WP_010968154.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - htpB 1..1354226 1354226


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T20642 WP_010968151.1 NC_003037:1249316-1249699 [Sinorhizobium meliloti 1021]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T20642 NC_003037:1249316-1249699 [Sinorhizobium meliloti 1021]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT20642 WP_013851145.1 NC_003037:1249119-1249319 [Sinorhizobium meliloti 1021]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT20642 NC_003037:1249119-1249319 [Sinorhizobium meliloti 1021]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References