Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1247456..1248036 Replicon plasmid pSymA
Accession NC_020527
Organism Sinorhizobium meliloti 2011

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag SM2011_RS06090 Protein ID WP_010968151.1
Coordinates 1247653..1248036 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag SM2011_RS06085 Protein ID WP_013851145.1
Coordinates 1247456..1247656 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM2011_RS06060 1242575..1243681 + 1107 WP_010968147.1 FAD-binding oxidoreductase -
SM2011_RS06065 1243671..1243985 + 315 WP_015445520.1 (2Fe-2S)-binding protein -
SM2011_RS06070 1243982..1245358 + 1377 WP_010968148.1 NAD(P)/FAD-dependent oxidoreductase -
SM2011_RS06075 1245450..1246223 - 774 WP_014528246.1 UTRA domain-containing protein -
SM2011_RS06080 1246414..1246941 - 528 WP_080590796.1 TIGR02444 family protein -
SM2011_RS06085 1247456..1247656 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SM2011_RS06090 1247653..1248036 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
SM2011_RS06095 1248488..1249267 - 780 WP_010968152.1 hypothetical protein -
SM2011_RS06100 1249493..1251157 + 1665 WP_015445521.1 hypothetical protein -
SM2011_RS06105 1251447..1252451 - 1005 WP_010968154.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - htpB 1..1352561 1352561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T29036 WP_010968151.1 NC_020527:1247653-1248036 [Sinorhizobium meliloti 2011]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T29036 NC_020527:1247653-1248036 [Sinorhizobium meliloti 2011]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT29036 WP_013851145.1 NC_020527:1247456-1247656 [Sinorhizobium meliloti 2011]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT29036 NC_020527:1247456-1247656 [Sinorhizobium meliloti 2011]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References