Detailed information of TA system TA077443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 953310..953917
• Replicon: plasmid psymA
• Accession: NZ_CP021798
• Organism: Sinorhizobium meliloti strain USDA1106

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDO30_RS23075
• Protein ID: WP_017264738.1
• Coordinates: 953663..953917 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: H0G1X1
• Locus tag: CDO30_RS23070
• Protein ID: WP_003530441.1
• Coordinates: 953310..953666 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDO30_RS23050	948817..950310	+	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-
CDO30_RS23055	950327..951298	+	972	WP_010968048.1	ABC transporter permease	-
CDO30_RS23060	951301..952251	+	951	WP_010968047.1	ABC transporter permease	-
CDO30_RS23065	952281..952937	+	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
CDO30_RS23070	953310..953666	-	357	WP_003530441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDO30_RS23075	953663..953917	-	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDO30_RS23080	954259..955146	-	888	WP_014528000.1	LysR family transcriptional regulator	-
CDO30_RS23085	955235..956275	+	1041	WP_010968044.1	NAD(P)H-binding protein	-
CDO30_RS34520	956399..956557	+	159	WP_017276730.1	hypothetical protein	-
CDO30_RS34525	956643..956798	+	156	WP_017264742.1	hypothetical protein	-
CDO30_RS23090	956849..957721	-	873	WP_010968043.1	LysR family transcriptional regulator	-
CDO30_RS23095	957812..958516	+	705	WP_010968042.1	haloacid dehalogenase type II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_003037 Sinorhizobium meliloti 1021 Conjugative plasmid TA006097, TA020640, TA020641, TA020642 1..1354226 0	inside	Conjugative plasmid	-	htpB	1..1363628	1363628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T77443 WP_017264738.1 NZ_CP021798:c953917-953663 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T77443 NZ_CP021798:c953917-953663 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13236.85 Da
• Isoelectric Point: 4.9920

>AT77443 WP_003530441.1 NZ_CP021798:c953666-953310 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHFAEARLSA

Nucleotide

• Length: 357 bp

>AT77443 NZ_CP021798:c953666-953310 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGACCATTTTGCCGAGGCGCGCCTGTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: H0G1X1