Detailed information of TA system TA029035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1127136..1127743
• Replicon: plasmid pSymA
• Accession: NC_020527
• Organism: Sinorhizobium meliloti 2011

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: SM2011_RS05490
• Protein ID: WP_017264738.1
• Coordinates: 1127136..1127390 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: H0G1X1
• Locus tag: SM2011_RS05495
• Protein ID: WP_003530441.1
• Coordinates: 1127387..1127743 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SM2011_RS05470	1122537..1123241	-	705	WP_010968042.1	haloacid dehalogenase type II	-
SM2011_RS05475	1123332..1124204	+	873	WP_010968043.1	LysR family transcriptional regulator	-
SM2011_RS35610	1124255..1124410	-	156	WP_017264742.1	hypothetical protein	-
SM2011_RS36095	1124496..1124654	-	159	WP_017276730.1	hypothetical protein	-
SM2011_RS05480	1124778..1125818	-	1041	WP_010968044.1	NAD(P)H-binding protein	-
SM2011_RS05485	1125907..1126794	+	888	WP_014528000.1	LysR family transcriptional regulator	-
SM2011_RS05490	1127136..1127390	+	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
SM2011_RS05495	1127387..1127743	+	357	WP_003530441.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SM2011_RS05500	1128116..1128772	-	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
SM2011_RS05505	1128802..1129752	-	951	WP_010968047.1	ABC transporter permease	-
SM2011_RS05510	1129755..1130726	-	972	WP_010968048.1	ABC transporter permease	-
SM2011_RS05515	1130743..1132236	-	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_003037 Sinorhizobium meliloti 1021 Conjugative plasmid TA006097, TA020640, TA020641, TA020642 1..1354226 0	inside	Conjugative plasmid	-	htpB	1..1352561	1352561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T29035 WP_017264738.1 NC_020527:1127136-1127390 [Sinorhizobium meliloti 2011]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T29035 NC_020527:1127136-1127390 [Sinorhizobium meliloti 2011]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13236.85 Da
• Isoelectric Point: 4.9920

>AT29035 WP_003530441.1 NC_020527:1127387-1127743 [Sinorhizobium meliloti 2011]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHFAEARLSA

Nucleotide

• Length: 357 bp

>AT29035 NC_020527:1127387-1127743 [Sinorhizobium meliloti 2011]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGACCATTTTGCCGAGGCGCGCCTGTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: H0G1X1