Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 64000..64453 | Replicon | plasmid pPsv48A |
| Accession | NC_019265 | ||
| Organism | Pseudomonas savastanoi | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | H1ZWW7 |
| Locus tag | PSA3335_RS00260 | Protein ID | WP_002556011.1 |
| Coordinates | 64000..64275 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q88BL7 |
| Locus tag | PSA3335_RS00265 | Protein ID | WP_002556012.1 |
| Coordinates | 64253..64453 (-) | Length | 67 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSA3335_RS00250 (PSA3335_A0048) | 62335..63557 | + | 1223 | WP_095178853.1 | IS3-like element IS51 family transposase | - |
| PSA3335_RS00255 | 63618..63956 | + | 339 | WP_168550533.1 | conjugal transfer protein TraR | - |
| PSA3335_RS00260 (PSA3335_A0050) | 64000..64275 | - | 276 | WP_002556011.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| PSA3335_RS00265 (PSA3335_A0051) | 64253..64453 | - | 201 | WP_002556012.1 | hypothetical protein | Antitoxin |
| PSA3335_RS00270 (PSA3335_A0052) | 64716..65297 | + | 582 | WP_244657731.1 | conjugal transfer protein TraT | - |
| PSA3335_RS00275 (PSA3335_A0053) | 65272..68328 | + | 3057 | WP_002556014.1 | conjugal transfer protein | - |
| PSA3335_RS00280 (PSA3335_A0054) | 68352..68588 | + | 237 | WP_002556015.1 | hypothetical protein | - |
| PSA3335_RS00285 (PSA3335_A0055) | 68610..68999 | + | 390 | WP_015060667.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..80123 | 80123 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10254.70 Da Isoelectric Point: 5.0357
>T10139 WP_002556011.1 NC_019265:c64275-64000 [Pseudomonas savastanoi pv. savastanoi]
VLTIRWADEASTDLVEIIDYIEQRSPIAAEALNANILKAVEGLPSAPYLFRHGREPGTRECVLHPNYLVVYQVGADTIDV
LRILHARQQYP
VLTIRWADEASTDLVEIIDYIEQRSPIAAEALNANILKAVEGLPSAPYLFRHGREPGTRECVLHPNYLVVYQVGADTIDV
LRILHARQQYP
Download Length: 276 bp
>T10139 NC_019265:c64275-64000 [Pseudomonas savastanoi pv. savastanoi]
GTGCTAACAATTCGCTGGGCTGATGAGGCCTCCACCGACTTGGTGGAAATTATCGACTACATCGAGCAGCGTAGCCCCAT
CGCCGCCGAGGCGCTGAACGCCAACATATTGAAGGCCGTTGAAGGCCTTCCATCAGCACCCTACCTGTTCCGCCACGGTC
GCGAACCCGGAACGCGTGAATGCGTTTTGCATCCCAACTACCTAGTGGTTTATCAGGTAGGGGCCGACACTATCGACGTG
CTGCGCATCCTACACGCCCGTCAGCAATACCCTTGA
GTGCTAACAATTCGCTGGGCTGATGAGGCCTCCACCGACTTGGTGGAAATTATCGACTACATCGAGCAGCGTAGCCCCAT
CGCCGCCGAGGCGCTGAACGCCAACATATTGAAGGCCGTTGAAGGCCTTCCATCAGCACCCTACCTGTTCCGCCACGGTC
GCGAACCCGGAACGCGTGAATGCGTTTTGCATCCCAACTACCTAGTGGTTTATCAGGTAGGGGCCGACACTATCGACGTG
CTGCGCATCCTACACGCCCGTCAGCAATACCCTTGA
Antitoxin
Download Length: 67 a.a. Molecular weight: 7523.42 Da Isoelectric Point: 8.6836
>AT10139 WP_002556012.1 NC_019265:c64453-64253 [Pseudomonas savastanoi pv. savastanoi]
MSTQLSPIVSEFETQEQADSYDRWFRAKVQEAMNSTKPRLPHDEAMAKVQTALAERRKARANNSLG
MSTQLSPIVSEFETQEQADSYDRWFRAKVQEAMNSTKPRLPHDEAMAKVQTALAERRKARANNSLG
Download Length: 201 bp
>AT10139 NC_019265:c64453-64253 [Pseudomonas savastanoi pv. savastanoi]
ATGAGCACCCAACTTTCCCCCATCGTTTCCGAGTTCGAAACACAGGAACAAGCAGACAGCTATGACCGCTGGTTTCGGGC
CAAGGTGCAGGAAGCCATGAACAGCACTAAGCCACGTTTGCCCCACGACGAAGCAATGGCGAAGGTGCAAACCGCCCTAG
CAGAGCGAAGGAAGGCCCGTGCTAACAATTCGCTGGGCTGA
ATGAGCACCCAACTTTCCCCCATCGTTTCCGAGTTCGAAACACAGGAACAAGCAGACAGCTATGACCGCTGGTTTCGGGC
CAAGGTGCAGGAAGCCATGAACAGCACTAAGCCACGTTTGCCCCACGACGAAGCAATGGCGAAGGTGCAAACCGCCCTAG
CAGAGCGAAGGAAGGCCCGTGCTAACAATTCGCTGGGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10141 | Pseudomonas savastanoi |
45.652 |
100 |
0.462 |
| T10143 | Pseudomonas savastanoi |
44.444 |
98.901 |
0.44 |
| T1006 | Escherichia coli O157:H7 str. Sakai |
40.217 |
100 |
0.407 |
| T1008 | Escherichia coli O157:H7 str. Sakai |
39.13 |
100 |
0.396 |
| T6097 | Sinorhizobium meliloti 1021 |
40.23 |
95.604 |
0.385 |
| T10119 | Escherichia coli |
36.957 |
100 |
0.374 |
| T6134 | Paracoccus aminophilus |
39.759 |
91.209 |
0.363 |
| T229 | Caulobacter crescentus CB15 |
34.568 |
89.011 |
0.308 |
| T705 | Vibrio parahaemolyticus RIMD 2210633 |
30.769 |
100 |
0.308 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10143 | Pseudomonas savastanoi |
57.407 |
81.818 |
0.47 |
| AT1006 | Escherichia coli O157:H7 str. Sakai |
52.727 |
87.302 |
0.46 |
| AT1008 | Escherichia coli O157:H7 str. Sakai |
45.902 |
96.825 |
0.444 |
| AT10141 | Pseudomonas savastanoi |
43.86 |
93.443 |
0.41 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3M5NBQ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3M5NAY4 |
References
(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866]