Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 39628..40106 | Replicon | plasmid pPsv48C |
| Accession | NC_019292 | ||
| Organism | Pseudomonas savastanoi | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | PSPSV_RS00250 | Protein ID | WP_002555994.1 |
| Coordinates | 39828..40106 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | H1ZX71 |
| Locus tag | PSPSV_RS00245 | Protein ID | WP_002555995.1 |
| Coordinates | 39628..39828 (+) | Length | 67 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSPSV_RS00225 (PSPSV_C0046) | 34713..35288 | + | 576 | WP_220789530.1 | hypothetical protein | - |
| PSPSV_RS00230 (PSPSV_C0047) | 35279..36607 | + | 1329 | WP_179894034.1 | hypothetical protein | - |
| PSPSV_RS00235 (PSPSV_C0048) | 36706..39036 | - | 2331 | WP_002555997.1 | MobQ family relaxase | - |
| PSPSV_RS00240 (PSPSV_C0049) | 39055..39300 | + | 246 | WP_002555996.1 | hypothetical protein | - |
| PSPSV_RS00245 (PSPSV_C0050) | 39628..39828 | + | 201 | WP_002555995.1 | hypothetical protein | Antitoxin |
| PSPSV_RS00250 (PSPSV_C0051) | 39828..40106 | + | 279 | WP_002555994.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| PSPSV_RS00255 (PSPSV_C0052) | 41480..41707 | + | 228 | WP_015060881.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..42104 | 42104 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10732.51 Da Isoelectric Point: 10.0464
>T10143 WP_002555994.1 NC_019292:39828-40106 [Pseudomonas savastanoi pv. savastanoi]
MQIIWRQRARMSLAKIIRYISNEDPQAAQAILERLQSAILPVADHPYLYRPGRVPGTRELVAHPNYVLVYRVTLERIEVV
NVIHARQEYPSL
MQIIWRQRARMSLAKIIRYISNEDPQAAQAILERLQSAILPVADHPYLYRPGRVPGTRELVAHPNYVLVYRVTLERIEVV
NVIHARQEYPSL
Download Length: 279 bp
>T10143 NC_019292:39828-40106 [Pseudomonas savastanoi pv. savastanoi]
ATGCAAATCATCTGGCGGCAACGTGCGCGCATGAGCCTGGCTAAAATCATCCGGTATATCTCCAACGAAGACCCGCAAGC
CGCCCAAGCGATTCTAGAGCGTTTACAGTCGGCCATACTGCCGGTGGCCGATCATCCATACCTGTACCGCCCTGGACGTG
TGCCAGGCACCCGCGAGCTGGTGGCCCACCCGAACTACGTCCTGGTCTACCGCGTCACGCTTGAGCGCATCGAGGTCGTG
AACGTAATCCACGCTAGGCAGGAATACCCCTCGCTCTAA
ATGCAAATCATCTGGCGGCAACGTGCGCGCATGAGCCTGGCTAAAATCATCCGGTATATCTCCAACGAAGACCCGCAAGC
CGCCCAAGCGATTCTAGAGCGTTTACAGTCGGCCATACTGCCGGTGGCCGATCATCCATACCTGTACCGCCCTGGACGTG
TGCCAGGCACCCGCGAGCTGGTGGCCCACCCGAACTACGTCCTGGTCTACCGCGTCACGCTTGAGCGCATCGAGGTCGTG
AACGTAATCCACGCTAGGCAGGAATACCCCTCGCTCTAA
Antitoxin
Download Length: 67 a.a. Molecular weight: 7450.22 Da Isoelectric Point: 4.0927
>AT10143 WP_002555995.1 NC_019292:39628-39828 [Pseudomonas savastanoi pv. savastanoi]
MANLSPIVSEFETDEQAASYDRWFRLQVQASLDDPSPGVPHDQVMAEMDAIIAEAEKRQQDRTKVS
MANLSPIVSEFETDEQAASYDRWFRLQVQASLDDPSPGVPHDQVMAEMDAIIAEAEKRQQDRTKVS
Download Length: 201 bp
>AT10143 NC_019292:39628-39828 [Pseudomonas savastanoi pv. savastanoi]
ATGGCCAACCTCTCACCCATCGTTTCGGAATTCGAAACCGATGAACAAGCAGCAAGCTACGACCGTTGGTTTCGGCTCCA
GGTGCAAGCATCACTGGACGATCCCAGTCCAGGTGTGCCGCACGATCAGGTCATGGCGGAAATGGACGCAATCATTGCCG
AGGCAGAAAAGCGTCAACAGGATCGGACTAAGGTCAGCTGA
ATGGCCAACCTCTCACCCATCGTTTCGGAATTCGAAACCGATGAACAAGCAGCAAGCTACGACCGTTGGTTTCGGCTCCA
GGTGCAAGCATCACTGGACGATCCCAGTCCAGGTGTGCCGCACGATCAGGTCATGGCGGAAATGGACGCAATCATTGCCG
AGGCAGAAAAGCGTCAACAGGATCGGACTAAGGTCAGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10141 | Pseudomonas savastanoi |
56.044 |
98.913 |
0.554 |
| T1008 | Escherichia coli O157:H7 str. Sakai |
49.451 |
98.913 |
0.489 |
| T1006 | Escherichia coli O157:H7 str. Sakai |
46.154 |
98.913 |
0.457 |
| T10139 | Pseudomonas savastanoi |
44.444 |
98.901 |
0.44 |
| T10119 | Escherichia coli |
38.043 |
100 |
0.38 |
| T6097 | Sinorhizobium meliloti 1021 |
38.043 |
100 |
0.38 |
| T229 | Caulobacter crescentus CB15 |
36.047 |
93.478 |
0.337 |
| T6134 | Paracoccus aminophilus |
35.227 |
95.652 |
0.337 |
| T10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
28 |
100 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1006 | Escherichia coli O157:H7 str. Sakai |
60.784 |
80.952 |
0.492 |
| AT10139 | Pseudomonas savastanoi |
57.407 |
81.818 |
0.47 |
| AT1008 | Escherichia coli O157:H7 str. Sakai |
51.852 |
85.714 |
0.444 |
| AT10141 | Pseudomonas savastanoi |
54.167 |
78.689 |
0.426 |
Multiple sequence alignment
References
(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866]