Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/- |
| Location | 2395..2942 | Replicon | plasmid pJIE512b |
| Accession | NC_025198 | ||
| Organism | Escherichia coli | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | G7QEJ9 |
| Locus tag | H4J97_RS00015 | Protein ID | WP_001384452.1 |
| Coordinates | 2664..2942 (+) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | K4JYR0 |
| Locus tag | H4J97_RS00010 | Protein ID | WP_000079928.1 |
| Coordinates | 2395..2664 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H4J97_RS00005 (D616_p157002) | 455..1486 | + | 1032 | WP_000907874.1 | plasmid replication initiator RepA | - |
| H4J97_RS00010 (D616_p157003) | 2395..2664 | + | 270 | WP_000079928.1 | type II toxin-antitoxin system antitoxin YacA | Antitoxin |
| H4J97_RS00015 (D616_p157004) | 2664..2942 | + | 279 | WP_001384452.1 | type II toxin-antitoxin system toxin YacB | Toxin |
| H4J97_RS00020 (D616_p157005) | 2982..3830 | + | 849 | WP_001057996.1 | 3'-5' exonuclease | - |
| H4J97_RS00025 (D616_p157006) | 3971..4420 | + | 450 | WP_001236003.1 | hypothetical protein | - |
| H4J97_RS00550 | 4897..5163 | - | 267 | Protein_5 | hypothetical protein | - |
| H4J97_RS00030 (D616_p157009) | 5163..5657 | - | 495 | WP_023278058.1 | hypothetical protein | - |
| H4J97_RS00035 (D616_p157010) | 5713..6315 | - | 603 | WP_000517689.1 | ProQ/FINO family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Conjugative plasmid | blaCMY-2 | - | 1..92339 | 92339 | ||
| flank | IS/Tn | blaCMY-2 | - | 6856..9795 | 2939 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10762.38 Da Isoelectric Point: 8.4615
>T10119 WP_001384452.1 NC_025198:2664-2942 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP
Download Length: 279 bp
>T10119 NC_025198:2664-2942 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG
Antitoxin
Download Length: 90 a.a. Molecular weight: 10175.50 Da Isoelectric Point: 7.3210
>AT10119 WP_000079928.1 NC_025198:2395-2664 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK
Download Length: 270 bp
>AT10119 NC_025198:2395-2664 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6097 | Sinorhizobium meliloti 1021 |
43.011 |
100 |
0.435 |
| T10143 | Pseudomonas savastanoi |
38.043 |
100 |
0.38 |
| T10139 | Pseudomonas savastanoi |
36.957 |
100 |
0.374 |
| T6134 | Paracoccus aminophilus |
40.244 |
89.13 |
0.359 |
| T1006 | Escherichia coli O157:H7 str. Sakai |
34.409 |
100 |
0.348 |
| T179 | Vibrio cholerae O1 biovar El Tor str. N16961 |
34.783 |
100 |
0.348 |
| T168 | Vibrio cholerae O1 biovar El Tor str. N16961 |
34.783 |
100 |
0.348 |
| T229 | Caulobacter crescentus CB15 |
36.364 |
95.652 |
0.348 |
| T10141 | Pseudomonas savastanoi |
33.333 |
100 |
0.337 |
| T178 | Vibrio cholerae O1 biovar El Tor str. N16961 |
33.721 |
93.478 |
0.315 |
| T6114 | uncultured bacterium clone 93 |
31.868 |
98.913 |
0.315 |
| T1008 | Escherichia coli O157:H7 str. Sakai |
30.108 |
100 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CL36 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5W2J4D0 |
References
(1) Muhammad Kamruzzaman et al. (2019) A ParDE-family toxin antitoxin system in major resistance plasmids of Enterobacteriaceae confers antibiotic and heat tolerance. Scientific Reports 9(1):9872. [PubMed:31285520]