1008

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/RHH-RelE
Location 2248879..2249386 Replicon chromosome
Accession NC_002695
Organism Escherichia coli O157:H7 str. Sakai

Toxin (Protein)


Gene name parE_1 Uniprot ID -
Locus tag ECs_2281 Protein ID NP_310308.1
Coordinates 2249244..2249522 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID -
Locus tag ECs_2280 Protein ID NP_310307.2
Coordinates 2249051..2249242 (+) Length 64 a.a.

Regulator (Protein)


Gene name paaR Uniprot ID -
Locus tag ECs_2279 Protein ID NP_310306.2
Coordinates 2248659..2249030 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECs_2271 2244038..2244142 - 105 NP_310298.1 hypothetical protein -
ECs_2272 2244258..2244842 - 585 NP_310299.1 hypothetical protein -
ECs_2273 2244899..2245294 - 396 NP_310300.1 phage exclusion protein -
ECs_2274 2245310..2245978 - 669 NP_310301.1 hypothetical protein -
ECs_2275 2246105..2246845 - 741 NP_310302.1 phage DNA replication protein -
ECs_2276 2246852..2247814 - 963 NP_310303.2 phage replication protein -
ECs_2277 2247837..2248262 - 426 NP_310304.1 hypothetical protein -
ECs_2278 2248259..2248561 - 303 NP_310305.1 phage antirepressor protein Cro -
ECs_2279 2248659..2249030 + 372 NP_310306.2 phage repressor protein CI Regulator
ECs_2280 2249051..2249242 + 192 NP_310307.2 hypothetical protein Antitoxin
ECs_2281 2249244..2249522 + 279 NP_310308.1 hypothetical protein Toxin
ECs_2282 2249953..2250153 - 201 NP_310309.2 hypothetical protein -
ECs_2283 2250246..2250464 + 219 NP_310310.2 hypothetical protein -
ECs_5735 2250468..2250632 - 165 YP_009502635.1 hypothetical protein -
ECs_2284 2251033..2251221 + 189 NP_310311.1 cell division inhibition protein -
ECs_2285 2251218..2251409 + 192 NP_310312.1 hypothetical protein -
ECs_2286 2251502..2253973 + 2472 NP_310313.1 exonuclease -
ECs_2287 2254061..2254297 + 237 NP_310314.2 phage excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2157035..2254487 97452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T1008 NP_310308.1 NC_002695:2249244-2249522 [Escherichia coli O157:H7 str. Sakai]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T1008 NC_002695:2249244-2249522 [Escherichia coli O157:H7 str. Sakai]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT1008 NP_310307.2 NC_002695:2249051-2249242 [Escherichia coli O157:H7 str. Sakai]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT1008 NC_002695:2249051-2249242 [Escherichia coli O157:H7 str. Sakai]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Regulator


Download         Length: 124 a.a.        Molecular weight: 14258.34 Da        Isoelectric Point: 9.7071

>RE1008 NP_310306.2 NC_002695:2248659-2249030 [Escherichia coli O157:H7 str. Sakai]
MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAARRIEQTYGMPEGYLDAEYAEQPGGSPPHAGLTSNQLE
LLQIFSAFPEDEQRQIISELKQKKESMEDLIARWIAAQKCRRA

Download         Length: 372 bp

>RE1008 NC_002695:2248659-2249030 [Escherichia coli O157:H7 str. Sakai]
ATGCAAAAAAAAGAAATTCGCCGTTTACGTCTCAAGGAGTGGTTTAAAGATAAAACTCTGCCACCCAAAGAGAAGAGCTA
CCTATCTCAACTAATGAGTGGGAGAGCCTCGTTTGGAGAAAAGGCTGCCAGAAGAATAGAGCAAACATACGGGATGCCGG
AAGGGTATCTGGATGCGGAATACGCAGAACAACCGGGGGGTTCTCCACCACATGCAGGGTTAACGTCTAATCAACTGGAA
TTATTGCAGATTTTTTCAGCCTTCCCTGAGGATGAGCAACGCCAGATAATCAGCGAGTTAAAGCAGAAAAAAGAATCAAT
GGAAGATCTCATAGCGAGATGGATTGCGGCGCAAAAATGCCGCCGCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10141 Pseudomonas savastanoi

71.739

100

0.717
T1006 Escherichia coli O157:H7 str. Sakai

67.391

100

0.674
T10143 Pseudomonas savastanoi

49.451

98.913

0.489
T10139 Pseudomonas savastanoi

39.13

100

0.396
T229 Caulobacter crescentus CB15

37.5

95.652

0.359
T178 Vibrio cholerae O1 biovar El Tor str. N16961

32.632

100

0.337
T6134 Paracoccus aminophilus

31.522

100

0.315
T10119 Escherichia coli

30.108

100

0.304

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10141 Pseudomonas savastanoi

69.643

91.803

0.639
AT1006 Escherichia coli O157:H7 str. Sakai

69.643

88.889

0.619
AT10139 Pseudomonas savastanoi

45.902

96.825

0.444
AT10143 Pseudomonas savastanoi

51.852

85.714

0.444

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Yann G-J Sterckx et al. (2016) A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. Journal of Molecular Biology 428(8):1589-603. [PubMed:26996937] 3D_structure

(2) Régis Hallez et al. (2010) New toxins homologous to ParE belonging to three-component toxin-antitoxin systems in Escherichia coli O157:H7. Molecular Microbiology 76(3):719-32. [PubMed:20345661] experimental

(3) Yann G J Sterckx et al. (2014) Crystallization and preliminary X-ray analysis of two variants of the Escherichia coli O157 ParE2-PaaA2 toxin-antitoxin complex. Acta Crystallographica. Section F, Structural Biology Communications 70(Pt 9):1284-91. [PubMed:25195911] 3D_structure

(4) Dukas Jurėnas et al. (2021) Bistable Expression of a Toxin-Antitoxin System Located in a Cryptic Prophage of Escherichia coli O157:H7. MBio 12(6):e0294721. [PubMed:34844426] experimental

(5) Yann G J Sterckx et al. (2012) The ParE2-PaaA2 toxin-antitoxin complex from Escherichia coli O157 forms a heterodocecamer in solution and in the crystal. Acta Crystallographica. Section F, Structural Biology And Crystallization Communications 68(Pt 6):724-9. [PubMed:22684081] 3D_structure


experimental experimental literature
3D_structure protein structure literature