Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2450259..2450730 Replicon chromosome
Accession NZ_CP028698
Organism Escherichia coli strain 106

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E35_RS12365 Protein ID WP_001303511.1
Coordinates 2450259..2450537 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E35_RS12370 Protein ID WP_001302048.1
Coordinates 2450539..2450730 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E35_RS12330 2445484..2445735 - 252 WP_000005552.1 excisionase family protein -
C9E35_RS12335 2445808..2448279 - 2472 WP_000048458.1 exonuclease -
C9E35_RS12340 2448372..2448563 - 192 WP_001090200.1 DUF1482 family protein -
C9E35_RS12345 2448560..2448748 - 189 WP_000449192.1 cell division inhibition protein DicB -
C9E35_RS12355 2449317..2449535 - 219 WP_001171930.1 hypothetical protein -
C9E35_RS12360 2449607..2449906 - 300 WP_001240334.1 hypothetical protein -
C9E35_RS12365 2450259..2450537 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E35_RS12370 2450539..2450730 - 192 WP_001302048.1 hypothetical protein Antitoxin
C9E35_RS12375 2450751..2451122 - 372 WP_001169686.1 hypothetical protein -
C9E35_RS12380 2451220..2451522 + 303 WP_000172738.1 transcriptional regulator -
C9E35_RS12385 2451519..2451944 + 426 WP_000693943.1 Rha family transcriptional regulator -
C9E35_RS12390 2451967..2452929 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E35_RS12395 2452936..2453676 + 741 WP_000788938.1 ATP-binding protein -
C9E35_RS12400 2453702..2454471 + 770 Protein_2379 DUF1627 domain-containing protein -
C9E35_RS12405 2454487..2454882 + 396 WP_001118159.1 DUF977 family protein -
C9E35_RS12410 2454939..2455523 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2435379..2548334 112955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102890 WP_001303511.1 NZ_CP028698:c2450537-2450259 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102890 NZ_CP028698:c2450537-2450259 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102890 WP_001302048.1 NZ_CP028698:c2450730-2450539 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102890 NZ_CP028698:c2450730-2450539 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References