Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2920027..2920498 Replicon chromosome
Accession NZ_CP038333
Organism Escherichia coli O157:H7 strain N8B7-2

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E4U68_RS15045 Protein ID WP_001303511.1
Coordinates 2920220..2920498 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E4U68_RS15040 Protein ID WP_001302048.1
Coordinates 2920027..2920218 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U68_RS15000 2915234..2915818 - 585 WP_000206793.1 DUF551 domain-containing protein -
E4U68_RS15005 2915875..2916270 - 396 WP_001118159.1 DUF977 family protein -
E4U68_RS15010 2916286..2917055 - 770 Protein_2940 DUF1627 domain-containing protein -
E4U68_RS15015 2917081..2917821 - 741 WP_000788936.1 ATP-binding protein -
E4U68_RS15020 2917828..2918790 - 963 WP_000095670.1 helix-turn-helix domain-containing protein -
E4U68_RS15025 2918813..2919238 - 426 WP_000693943.1 Rha family transcriptional regulator -
E4U68_RS15030 2919235..2919537 - 303 WP_000172738.1 transcriptional regulator -
E4U68_RS15035 2919635..2920006 + 372 WP_001169686.1 hypothetical protein -
E4U68_RS15040 2920027..2920218 + 192 WP_001302048.1 hypothetical protein Antitoxin
E4U68_RS15045 2920220..2920498 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E4U68_RS15050 2920851..2921150 + 300 WP_001240334.1 hypothetical protein -
E4U68_RS15055 2921222..2921440 + 219 WP_001171930.1 hypothetical protein -
E4U68_RS15060 2921444..2921608 - 165 WP_001331716.1 hypothetical protein -
E4U68_RS15065 2922009..2922197 + 189 WP_000449192.1 cell division inhibition protein DicB -
E4U68_RS15070 2922194..2922385 + 192 WP_001090200.1 DUF1482 family protein -
E4U68_RS15075 2922478..2924949 + 2472 WP_000048458.1 exonuclease -
E4U68_RS15080 2925022..2925273 + 252 WP_000005552.1 excisionase family protein -
E4U68_RS15085 2925308..2925495 + 188 Protein_2955 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2829669..2927659 97990


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T121554 WP_001303511.1 NZ_CP038333:2920220-2920498 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T121554 NZ_CP038333:2920220-2920498 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT121554 WP_001302048.1 NZ_CP038333:2920027-2920218 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT121554 NZ_CP038333:2920027-2920218 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References