140234

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4993350..4993821 Replicon chromosome
Accession NZ_CP044143
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag F4V41_RS25945 Protein ID WP_001303511.1
Coordinates 4993350..4993628 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag F4V41_RS25950 Protein ID WP_001302048.1
Coordinates 4993630..4993821 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS25910 4988575..4988826 - 252 WP_000005552.1 excisionase family protein -
F4V41_RS25915 4988899..4991370 - 2472 WP_000048458.1 exonuclease -
F4V41_RS25920 4991463..4991654 - 192 WP_001090200.1 DUF1482 family protein -
F4V41_RS25925 4991651..4991839 - 189 WP_000449192.1 cell division inhibition protein DicB -
F4V41_RS25935 4992408..4992626 - 219 WP_001171930.1 hypothetical protein -
F4V41_RS25940 4992698..4992997 - 300 WP_001240334.1 hypothetical protein -
F4V41_RS25945 4993350..4993628 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F4V41_RS25950 4993630..4993821 - 192 WP_001302048.1 hypothetical protein Antitoxin
F4V41_RS25955 4993842..4994213 - 372 WP_001169686.1 hypothetical protein -
F4V41_RS25960 4994311..4994613 + 303 WP_000172738.1 transcriptional regulator -
F4V41_RS25965 4994610..4995035 + 426 WP_000693943.1 Rha family transcriptional regulator -
F4V41_RS25970 4995058..4996020 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
F4V41_RS25975 4996027..4996767 + 741 WP_000788938.1 ATP-binding protein -
F4V41_RS25980 4996793..4997562 + 770 Protein_4748 DUF1627 domain-containing protein -
F4V41_RS25985 4997578..4997973 + 396 WP_001118159.1 DUF977 family protein -
F4V41_RS25990 4998030..4998614 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 4978470..5089516 111046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T140234 WP_001303511.1 NZ_CP044143:c4993628-4993350 [Escherichia coli O157]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T140234 NZ_CP059044:238819-239235 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT140234 WP_001302048.1 NZ_CP044143:c4993821-4993630 [Escherichia coli O157]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT140234 NZ_CP059044:238592-238822 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References