122663

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2561684..2562155 Replicon chromosome
Accession NZ_CP038421
Organism Escherichia coli O157:H7 strain 7636

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3152_RS12830 Protein ID WP_001303511.1
Coordinates 2561684..2561962 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3152_RS12835 Protein ID WP_001302048.1
Coordinates 2561964..2562155 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3152_RS12790 2556690..2556874 - 185 Protein_2502 DUF3596 domain-containing protein -
E3152_RS12795 2556909..2557160 - 252 WP_000005552.1 excisionase family protein -
E3152_RS12800 2557233..2559704 - 2472 WP_000048458.1 exonuclease -
E3152_RS12805 2559797..2559988 - 192 WP_001090200.1 DUF1482 family protein -
E3152_RS12810 2559985..2560173 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3152_RS12815 2560574..2560738 + 165 WP_001331716.1 hypothetical protein -
E3152_RS12820 2560742..2560960 - 219 WP_001171930.1 hypothetical protein -
E3152_RS12825 2561032..2561331 - 300 WP_072143447.1 hypothetical protein -
E3152_RS12830 2561684..2561962 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3152_RS12835 2561964..2562155 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3152_RS12840 2562176..2562547 - 372 WP_001169686.1 hypothetical protein -
E3152_RS12845 2562645..2562947 + 303 WP_000172738.1 transcriptional regulator -
E3152_RS12850 2562944..2563369 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3152_RS12855 2563392..2564354 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3152_RS12860 2564361..2565101 + 741 WP_000788938.1 ATP-binding protein -
E3152_RS12865 2565127..2565896 + 770 Protein_2517 DUF1627 domain-containing protein -
E3152_RS12870 2565912..2566307 + 396 WP_001118159.1 DUF977 family protein -
E3152_RS12875 2566364..2566948 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleG7' 2541676..2659399 117723


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122663 WP_001303511.1 NZ_CP038421:c2561962-2561684 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122663 NZ_CP038421:c2561962-2561684 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122663 WP_001302048.1 NZ_CP038421:c2562155-2561964 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122663 NZ_CP038421:c2562155-2561964 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References