1006

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/RHH-RelE
Location 1167455..1167933 Replicon chromosome
Accession NC_002695
Organism Escherichia coli O157:H7 str. Sakai

Toxin (Protein)


Gene name parE_1 Uniprot ID -
Locus tag ECs_1067 Protein ID NP_309094.1
Coordinates 1167456..1167743 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID -
Locus tag ECs_1068 Protein ID NP_309095.1
Coordinates 1167743..1167934 (-) Length 64 a.a.

Regulator (Protein)


Gene name paaR Uniprot ID -
Locus tag ECs_1069 Protein ID NP_309096.2
Coordinates 1167962..1168363 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECs_1057 1162605..1165076 - 2472 NP_309084.1 exonuclease -
ECs_1058 1165170..1165361 - 192 NP_309085.1 hypothetical protein -
ECs_1059 1165358..1165546 - 189 NP_309086.1 cell division inhibition protein -
ECs_1061 1166120..1166305 + 186 NP_309088.1 hypothetical protein -
ECs_1063 1166492..1166881 - 390 NP_309090.1 hypothetical protein -
ECs_1064 1166893..1167021 - 129 NP_303797.1 hypothetical protein -
ECs_1065 1167023..1167178 - 156 NP_309092.1 hypothetical protein -
ECs_1067 1167456..1167743 - 288 NP_309094.1 plasmid stabilization system protein Toxin
ECs_1068 1167743..1167934 - 192 NP_309095.1 hypothetical protein Antitoxin
ECs_1069 1167962..1168363 - 402 NP_309096.2 phage repressor protein Regulator
ECs_1070 1168472..1168744 + 273 NP_309097.1 hypothetical protein -
ECs_1071 1168728..1169153 + 426 NP_309098.1 hypothetical protein -
ECs_5703 1169360..1169815 - 456 YP_009502597.1 hypothetical protein -
ECs_1073 1169894..1170985 + 1092 NP_309100.1 phage replication protein -
ECs_1074 1170992..1171738 + 747 NP_309101.1 phage DNA replication protein -
ECs_1075 1171760..1172530 + 771 NP_309102.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10978.84 Da        Isoelectric Point: 10.1360

>T1006 NP_309094.1 NC_002695:c1167743-1167456 [Escherichia coli O157:H7 str. Sakai]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Download         Length: 288 bp

>T1006 NC_002695:c1167743-1167456 [Escherichia coli O157:H7 str. Sakai]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7025.80 Da        Isoelectric Point: 4.0270

>AT1006 NP_309095.1 NC_002695:c1167934-1167743 [Escherichia coli O157:H7 str. Sakai]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Download         Length: 192 bp

>AT1006 NC_002695:c1167934-1167743 [Escherichia coli O157:H7 str. Sakai]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA

Regulator


Download         Length: 134 a.a.        Molecular weight: 15301.65 Da        Isoelectric Point: 8.0365

>RE1006 NP_309096.2 NC_002695:c1168363-1167962 [Escherichia coli O157:H7 str. Sakai]
MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGKATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPD
TMKIGPRQRELLQAFSAFPEEDQEKMLQEIKDKKKSMEETIARWLAAQKSRRA

Download         Length: 402 bp

>RE1006 NC_002695:c1168363-1167962 [Escherichia coli O157:H7 str. Sakai]
ATGATGAGCGCCCTAGAAGTATCTATGTACAGAATCAGCAAGCTTCTTCAGGAAACTGGATGGAGCCAGGCTGAGCTTGC
CCGTAGAATTGGTGTGACACAACAAACTGTTCAACAATGGGTCAGCGGTAAGGCTACACCTAAAGCCTCAAGTTTGGATA
AACTGGTTGAGGTTTCAGGGCATCCATTGCATTGGTTTTTATTGCCTCCTGAAGAGTGTGAGCAAATTTTCACCCCTGAC
ACGATGAAAATTGGTCCTCGCCAACGCGAACTGCTCCAAGCTTTTAGTGCGTTTCCTGAGGAAGACCAAGAAAAAATGCT
TCAAGAAATCAAAGACAAGAAAAAATCAATGGAAGAAACCATTGCTAGGTGGCTGGCGGCACAAAAAAGCCGCCGGGCGT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1008 Escherichia coli O157:H7 str. Sakai

67.391

100

0.674
T10141 Pseudomonas savastanoi

56.522

96.842

0.547
T10143 Pseudomonas savastanoi

46.154

98.913

0.457
T10139 Pseudomonas savastanoi

40.217

100

0.407
T10119 Escherichia coli

34.409

100

0.348
T229 Caulobacter crescentus CB15

36.364

92.632

0.337
T6097 Sinorhizobium meliloti 1021

33.696

96.842

0.326
T178 Vibrio cholerae O1 biovar El Tor str. N16961

31.183

97.895

0.305

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1008 Escherichia coli O157:H7 str. Sakai

69.643

88.889

0.619
AT10141 Pseudomonas savastanoi

60.784

83.607

0.508
AT10143 Pseudomonas savastanoi

60.784

80.952

0.492
AT10139 Pseudomonas savastanoi

52.727

87.302

0.46

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References

(1) Régis Hallez et al. (2010) New toxins homologous to ParE belonging to three-component toxin-antitoxin systems in Escherichia coli O157:H7. Molecular Microbiology 76(3):719-32. [PubMed:20345661] experimental


experimental experimental literature