Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/- |
| Location | 1920..2398 | Replicon | plasmid pPsv48C |
| Accession | NC_019292 | ||
| Organism | Pseudomonas savastanoi | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | H1ZX33 |
| Locus tag | PSPSV_RS00020 | Protein ID | WP_003348565.1 |
| Coordinates | 2105..2398 (+) | Length | 98 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | - |
| Locus tag | PSPSV_RS00015 | Protein ID | WP_122267529.1 |
| Coordinates | 1920..2105 (+) | Length | 62 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PSPSV_RS00005 (PSPSV_C0001) | 1..1314 | + | 1314 | WP_015060860.1 | replication initiation protein | - |
| PSPSV_RS00010 (PSPSV_C0002) | 1540..1833 | + | 294 | WP_002556165.1 | HU family DNA-binding protein | - |
| PSPSV_RS00015 (PSPSV_C0003) | 1920..2105 | + | 186 | WP_122267529.1 | stability determinant | Antitoxin |
| PSPSV_RS00020 (PSPSV_C0004) | 2105..2398 | + | 294 | WP_003348565.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| PSPSV_RS00025 (PSPSV_C0005) | 2508..3392 | - | 885 | Protein_4 | IS3-like element IS51 family transposase | - |
| PSPSV_RS00030 (PSPSV_C0006) | 3440..3973 | - | 534 | WP_057425675.1 | hypothetical protein | - |
| PSPSV_RS00035 (PSPSV_C0007) | 3979..4272 | - | 294 | WP_002556167.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| PSPSV_RS00040 (PSPSV_C0008) | 4260..4532 | - | 273 | WP_002556168.1 | CopG family ribbon-helix-helix protein | - |
| PSPSV_RS00045 (PSPSV_C0010) | 4800..4922 | - | 123 | Protein_8 | stability/partitioning determinant | - |
| PSPSV_RS00050 | 5155..5529 | - | 375 | Protein_9 | integrase core domain-containing protein | - |
| PSPSV_RS00055 (PSPSV_C0013) | 5579..6801 | + | 1223 | WP_168550525.1 | IS3-like element IS51 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..42104 | 42104 | |
| - | inside | IScluster/Tn | - | - | 2508..20294 | 17786 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 98 a.a. Molecular weight: 11136.84 Da Isoelectric Point: 6.2150
>T10141 WP_003348565.1 NC_019292:2105-2398 [Pseudomonas savastanoi pv. savastanoi]
MLPIFWLESADNDLAAIIEYIGSRDIAAAERLWQRLRGIVLPLSEHPYLYAISDRVPGMREIVAHPNYLVFYRVTSTRIE
VVNVVHARQEYPQTGLA
MLPIFWLESADNDLAAIIEYIGSRDIAAAERLWQRLRGIVLPLSEHPYLYAISDRVPGMREIVAHPNYLVFYRVTSTRIE
VVNVVHARQEYPQTGLA
Download Length: 294 bp
>T10141 NC_019292:2105-2398 [Pseudomonas savastanoi pv. savastanoi]
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTCACGTGATATAGC
TGCTGCCGAAAGGTTATGGCAGCGCTTGCGTGGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTCCCAGGAATGCGCGAGATCGTTGCCCATCCTAACTACCTGGTGTTTTATCGCGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCACGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG
ATGCTACCCATTTTCTGGCTAGAATCAGCGGACAACGATTTGGCGGCAATAATCGAATACATAGGTTCACGTGATATAGC
TGCTGCCGAAAGGTTATGGCAGCGCTTGCGTGGCATCGTTCTGCCCCTTTCAGAACATCCTTATCTGTATGCGATAAGTG
ACCGCGTCCCAGGAATGCGCGAGATCGTTGCCCATCCTAACTACCTGGTGTTTTATCGCGTCACCTCAACGCGCATAGAA
GTGGTGAACGTCGTCCACGCACGGCAAGAATATCCCCAAACTGGACTAGCGTAG
Antitoxin
Download Length: 62 a.a. Molecular weight: 7178.09 Da Isoelectric Point: 6.4675
>AT10141 WP_122267529.1 NC_019292:1920-2105 [Pseudomonas savastanoi pv. savastanoi]
MIDRSPIVSEFETEELEANYTVWLRAKVEASLADSRPAIPHDEVERRMAERLSRLRHRRAS
MIDRSPIVSEFETEELEANYTVWLRAKVEASLADSRPAIPHDEVERRMAERLSRLRHRRAS
Download Length: 186 bp
>AT10141 NC_019292:1920-2105 [Pseudomonas savastanoi pv. savastanoi]
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGTCTGGCTACGCGCGAA
GGTGGAGGCCAGTCTGGCCGACTCTCGCCCTGCTATCCCGCATGATGAAGTCGAGCGAAGGATGGCAGAGCGGTTGTCAC
GATTGCGCCATCGCCGAGCGTCCTGA
ATGATTGATCGTTCGCCTATCGTTTCGGAGTTTGAAACTGAAGAGCTAGAGGCCAATTACACCGTCTGGCTACGCGCGAA
GGTGGAGGCCAGTCTGGCCGACTCTCGCCCTGCTATCCCGCATGATGAAGTCGAGCGAAGGATGGCAGAGCGGTTGTCAC
GATTGCGCCATCGCCGAGCGTCCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T1008 | Escherichia coli O157:H7 str. Sakai |
71.739 |
100 |
0.717 |
| T10143 | Pseudomonas savastanoi |
56.044 |
98.913 |
0.554 |
| T1006 | Escherichia coli O157:H7 str. Sakai |
56.522 |
96.842 |
0.547 |
| T10139 | Pseudomonas savastanoi |
45.652 |
100 |
0.462 |
| T10119 | Escherichia coli |
33.333 |
100 |
0.337 |
| T178 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.292 |
98.969 |
0.32 |
| T10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
31.915 |
96.907 |
0.309 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT1008 | Escherichia coli O157:H7 str. Sakai |
69.643 |
91.803 |
0.639 |
| AT1006 | Escherichia coli O157:H7 str. Sakai |
60.784 |
83.607 |
0.508 |
| AT10143 | Pseudomonas savastanoi |
54.167 |
78.689 |
0.426 |
| AT10139 | Pseudomonas savastanoi |
43.86 |
93.443 |
0.41 |
Multiple sequence alignment
References
(1) Leire Bardaji et al. (2019) The toxic guardians - multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids. Mobile DNA 10:7. [PubMed:30728866]