Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prpT-prpA/- |
| Location | 23995..24541 | Replicon | plasmid pMBL6842 |
| Accession | NZ_CP013613 | ||
| Organism | Pseudoalteromonas rubra strain SCSIO 6842 | ||
Toxin (Protein)
| Gene name | PrpT | Uniprot ID | A0A0U2Y7P6 |
| Locus tag | AT705_RS24520 | Protein ID | WP_058798965.1 |
| Coordinates | 23995..24291 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | PrpA | Uniprot ID | A0A0U3H4C4 |
| Locus tag | AT705_RS24525 | Protein ID | WP_058798966.1 |
| Coordinates | 24281..24541 (-) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AT705_RS24500 (AT705_24500) | 20050..21162 | + | 1113 | WP_058798961.1 | toprim domain-containing protein | - |
| AT705_RS24505 (AT705_24505) | 21500..21763 | + | 264 | WP_058798962.1 | hypothetical protein | - |
| AT705_RS24510 (AT705_24510) | 22527..23483 | + | 957 | WP_058798963.1 | tyrosine-type recombinase/integrase | - |
| AT705_RS24515 (AT705_24515) | 23610..23975 | + | 366 | WP_058798964.1 | hypothetical protein | - |
| AT705_RS24520 (AT705_24520) | 23995..24291 | - | 297 | WP_058798965.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| AT705_RS24525 (AT705_24525) | 24281..24541 | - | 261 | WP_058798966.1 | type II toxin-antitoxin system ParD family antitoxin | Antitoxin |
| AT705_RS24530 (AT705_24530) | 24628..25047 | - | 420 | WP_058798967.1 | OmpA family protein | - |
| AT705_RS24535 (AT705_24535) | 25056..25550 | - | 495 | WP_058798968.1 | hypothetical protein | - |
| AT705_RS24540 (AT705_24540) | 25763..26728 | + | 966 | WP_058798969.1 | hypothetical protein | - |
| AT705_RS24545 (AT705_24545) | 26747..27376 | - | 630 | WP_058798970.1 | hypothetical protein | - |
| AT705_RS25550 | 27351..27647 | - | 297 | WP_167552028.1 | hypothetical protein | - |
| AT705_RS24555 (AT705_24555) | 28238..28951 | + | 714 | WP_058798972.1 | EAL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..69868 | 69868 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11269.71 Da Isoelectric Point: 5.5010
>T10079 WP_058798965.1 NZ_CP013613:c24291-23995 [Pseudoalteromonas rubra]
VVANTLVLKPRAEQDLERIFEYSYTEFGWQQAQQYISDLDQTFQTLAASTDLAINYDHVRPGLKAFPVGAHIVFFRATDT
GIEVIRVLHQSMDYPRHV
VVANTLVLKPRAEQDLERIFEYSYTEFGWQQAQQYISDLDQTFQTLAASTDLAINYDHVRPGLKAFPVGAHIVFFRATDT
GIEVIRVLHQSMDYPRHV
Download Length: 297 bp
>T10079 NZ_CP013613:c24291-23995 [Pseudoalteromonas rubra]
GTGGTGGCTAATACTCTGGTCCTCAAACCCCGTGCGGAACAAGATTTAGAACGCATTTTTGAATACAGCTATACGGAGTT
TGGCTGGCAACAGGCGCAGCAGTATATCAGCGATCTGGACCAGACCTTTCAGACACTCGCGGCATCGACCGATCTGGCTA
TTAATTACGATCATGTCCGTCCCGGCCTGAAAGCCTTTCCGGTCGGCGCCCATATCGTATTCTTCCGCGCCACAGACACC
GGCATCGAAGTGATCCGGGTGCTGCATCAATCCATGGACTACCCACGTCATGTGTGA
GTGGTGGCTAATACTCTGGTCCTCAAACCCCGTGCGGAACAAGATTTAGAACGCATTTTTGAATACAGCTATACGGAGTT
TGGCTGGCAACAGGCGCAGCAGTATATCAGCGATCTGGACCAGACCTTTCAGACACTCGCGGCATCGACCGATCTGGCTA
TTAATTACGATCATGTCCGTCCCGGCCTGAAAGCCTTTCCGGTCGGCGCCCATATCGTATTCTTCCGCGCCACAGACACC
GGCATCGAAGTGATCCGGGTGCTGCATCAATCCATGGACTACCCACGTCATGTGTGA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9499.63 Da Isoelectric Point: 4.8537
>AT10079 WP_058798966.1 NZ_CP013613:c24541-24281 [Pseudoalteromonas rubra]
MSSRTMTVDTGEELRAFVEGLVESGDYKTNSEVIRDGLRLLQEKTAGSKLAALRQLIDEGEQSGEAVPWDRDSFLARMRQ
KGPRGG
MSSRTMTVDTGEELRAFVEGLVESGDYKTNSEVIRDGLRLLQEKTAGSKLAALRQLIDEGEQSGEAVPWDRDSFLARMRQ
KGPRGG
Download Length: 261 bp
>AT10079 NZ_CP013613:c24541-24281 [Pseudoalteromonas rubra]
ATGTCATCTCGAACTATGACGGTTGATACCGGTGAAGAATTGCGCGCCTTTGTTGAAGGCCTGGTGGAATCTGGCGACTA
TAAGACCAACAGCGAAGTGATCCGCGATGGGCTCCGTCTGCTGCAGGAAAAAACCGCCGGGTCTAAACTGGCAGCATTGC
GCCAGCTCATTGATGAAGGTGAGCAAAGTGGCGAAGCTGTGCCCTGGGATCGAGATAGCTTTTTAGCCCGCATGAGGCAA
AAAGGCCCGCGTGGTGGCTAA
ATGTCATCTCGAACTATGACGGTTGATACCGGTGAAGAATTGCGCGCCTTTGTTGAAGGCCTGGTGGAATCTGGCGACTA
TAAGACCAACAGCGAAGTGATCCGCGATGGGCTCCGTCTGCTGCAGGAAAAAACCGCCGGGTCTAAACTGGCAGCATTGC
GCCAGCTCATTGATGAAGGTGAGCAAAGTGGCGAAGCTGTGCCCTGGGATCGAGATAGCTTTTTAGCCCGCATGAGGCAA
AAAGGCCCGCGTGGTGGCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T105 | Mycobacterium tuberculosis H37Rv |
36.735 |
100 |
0.367 |
| T228 | Caulobacter crescentus CB15 |
35.165 |
94.792 |
0.333 |
| T10141 | Pseudomonas savastanoi |
31.915 |
96.907 |
0.309 |
| T169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
36.145 |
84.694 |
0.306 |
| T10143 | Pseudomonas savastanoi |
28 |
100 |
0.304 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT228 | Caulobacter crescentus CB15 |
45.349 |
100 |
0.453 |
| AT169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
40.244 |
100 |
0.413 |
| AT105 | Mycobacterium tuberculosis H37Rv |
43.243 |
89.157 |
0.386 |
| AT233 | Caulobacter crescentus CB15 |
45 |
72.289 |
0.325 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 7YCS | |
| PDB | 7YCU |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0U3H4C4 |
References
(1) Songwei Ni et al. (2021) Conjugative plasmid-encoded toxin-antitoxin system PrpT/PrpA directly controls plasmid copy number. Proceedings of The National Academy of Sciences of The United States of America 118(4):e2011577118. [PubMed:33483419]