Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) parDE/RHH-RelE
Location 3202309..3202882 Replicon chromosome
Accession NC_002696
Organism Caulobacter crescentus CB15

Toxin (Protein)


Gene name parE Uniprot ID A0A0H3CBC5
Locus tag CC_RS15575 Protein ID WP_012640626.1
Coordinates 3202309..3202644 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A0H3CC80
Locus tag CC_RS15580 Protein ID WP_024265886.1
Coordinates 3202592..3202843 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CC_RS15545 (CC_2978) 3197657..3198181 + 525 WP_010920815.1 DUF3429 domain-containing protein -
CC_RS15550 3198181..3198903 + 723 WP_012640625.1 SDR family oxidoreductase -
CC_RS15555 (CC_2980) 3198900..3199451 + 552 WP_010920817.1 Lrp/AsnC family transcriptional regulator -
CC_RS15560 (CC_2981) 3199545..3201794 + 2250 WP_010920818.1 excinuclease ABC subunit UvrB -
CC_RS15565 3201973..3202158 + 186 WP_024265885.1 hypothetical protein -
CC_RS15570 (CC_2983) 3202160..3202273 + 114 WP_010920820.1 type II toxin-antitoxin system VapB family antitoxin -
CC_RS15575 (CC_2984) 3202309..3202644 - 336 WP_012640626.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CC_RS15580 (CC_2985) 3202592..3202843 - 252 WP_024265886.1 type II toxin-antitoxin system ParD family antitoxin Antitoxin
CC_RS15585 3202861..3203040 - 180 WP_024265887.1 hypothetical protein -
CC_RS15590 (CC_2986) 3203079..3203486 - 408 WP_024265888.1 lysozyme inhibitor LprI family protein -
CC_RS15595 (CC_2987) 3203860..3204612 - 753 WP_024265889.1 hypothetical protein -
CC_RS15600 3204903..3205052 - 150 Protein_3063 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase -
CC_RS15605 (CC_2988) 3205343..3207067 - 1725 WP_010920825.1 response regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(17-105)

Antitoxin

(6-62)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12367.30 Da        Isoelectric Point: 8.9620

>T233 WP_012640626.1 NC_002696:c3202644-3202309 [Caulobacter vibrioides CB15]
MRFALRRGGGLVWIMSYRLSRKAEQDLIDIYVAGVGLFGVAQAERYQDTLEAAFGAIAAFPHIGRERPELRPPVRVHPCK
SHIILYVLDERGALIVRVRHAGEDWVGEAGG

Download         Length: 336 bp

>T233 NC_002696:c3202644-3202309 [Caulobacter vibrioides CB15]
ATGAGATTCGCGCTGAGGCGCGGCGGCGGGCTGGTGTGGATCATGAGCTATAGGCTCAGCCGGAAGGCCGAGCAGGACCT
GATCGACATCTATGTCGCCGGCGTCGGCCTGTTCGGCGTCGCCCAGGCCGAGCGGTATCAGGACACGCTGGAAGCGGCCT
TCGGCGCCATCGCCGCCTTCCCGCACATTGGCCGCGAGCGCCCCGAACTCCGGCCGCCGGTGCGTGTGCACCCGTGCAAG
TCTCACATCATCCTGTACGTGCTGGACGAGCGCGGAGCGCTGATCGTGCGCGTGCGGCACGCAGGGGAGGATTGGGTCGG
AGAGGCCGGCGGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9569.61 Da        Isoelectric Point: 6.0817

>AT233 WP_024265886.1 NC_002696:c3202843-3202592 [Caulobacter vibrioides CB15]
MATMNVSLPDAMREWVEGQTQSGRYHNASEYVRDLIRRDQERADKIAHLQRLIDEGLDSGVGERSLHEIRAEARRRAGVD
HEL

Download         Length: 252 bp

>AT233 NC_002696:c3202843-3202592 [Caulobacter vibrioides CB15]
ATGGCGACGATGAATGTTTCCCTGCCCGATGCGATGCGCGAGTGGGTCGAGGGCCAGACCCAGTCGGGCCGCTATCACAA
CGCCAGCGAATACGTCCGTGACCTCATCCGTCGCGACCAGGAACGCGCCGACAAGATCGCCCACCTGCAACGCCTGATCG
ACGAAGGCCTAGACAGCGGCGTTGGCGAGCGCTCGCTGCATGAGATTCGCGCTGAGGCGCGGCGGCGGGCTGGTGTGGAT
CATGAGCTATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T105 Mycobacterium tuberculosis H37Rv

37.079

90.816

0.337

T228 Caulobacter crescentus CB15

38.095

87.5

0.333


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT228 Caulobacter crescentus CB15

35.443

95.181

0.337

AT10079 Pseudoalteromonas rubra strain SCSIO 6842

45

72.289

0.325


Multiple sequence alignment    




Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3CBC5


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3CC80

References


(1) Aretha Fiebig et al. (2010) Interaction specificity, toxicity and regulation of a paralogous set of ParE/RelE-family toxin-antitoxin systems. Molecular Microbiology 77(1):236-51. [PubMed:20487277] experimental


experimental experimental literature