Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RHH-RelE |
| Location | 968678..969231 | Replicon | chromosome |
| Accession | NC_002696 | ||
| Organism | Caulobacter crescentus CB15 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | Q9A9T8 |
| Locus tag | CC_RS04550 | Protein ID | WP_010918758.1 |
| Coordinates | 968678..968968 (-) | Length | 97 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | P58091 |
| Locus tag | CC_RS04555 | Protein ID | WP_010918759.1 |
| Coordinates | 968965..969231 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CC_RS04520 (CC_0867) | 964045..965235 | - | 1191 | WP_010918752.1 | erythromycin esterase family protein | - |
| CC_RS04525 (CC_0868) | 965298..965888 | - | 591 | WP_010918753.1 | AAA family ATPase | - |
| CC_RS04530 (CC_0869) | 965885..966952 | - | 1068 | WP_012640075.1 | serine hydrolase | - |
| CC_RS04535 (CC_0870) | 967002..967178 | - | 177 | WP_010918755.1 | hypothetical protein | - |
| CC_RS04540 (CC_0871) | 967257..967784 | - | 528 | WP_012640076.1 | GNAT family N-acetyltransferase | - |
| CC_RS04545 (CC_0872) | 967916..968677 | + | 762 | WP_024265642.1 | MOSC domain-containing protein | - |
| CC_RS04550 (CC_0873) | 968678..968968 | - | 291 | WP_010918758.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CC_RS04555 (CC_0874) | 968965..969231 | - | 267 | WP_010918759.1 | type II toxin-antitoxin system ParD family antitoxin | Antitoxin |
| CC_RS04560 (CC_0875) | 969305..970174 | + | 870 | WP_012640077.1 | peptide chain release factor N(5)-glutamine methyltransferase | - |
| CC_RS04565 | 970212..970436 | + | 225 | WP_012640078.1 | hypothetical protein | - |
| CC_RS04570 (CC_0876) | 970433..971545 | + | 1113 | WP_010918761.1 | DUF4167 domain-containing protein | - |
| CC_RS04575 (CC_0877) | 971713..972468 | + | 756 | WP_010918762.1 | M48 family metallopeptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 97 a.a. Molecular weight: 11057.55 Da Isoelectric Point: 8.9111
>T228 WP_010918758.1 NC_002696:c968968-968678 [Caulobacter vibrioides CB15]
MKPYRLSRRAKADLDDIWTYSEQRWGVEQAADYARELQATIEMIAEHPGMGQPDENLRAGYRRCASGSHVVFYRVGVRVE
IIRVLHQSMNARAHLG
MKPYRLSRRAKADLDDIWTYSEQRWGVEQAADYARELQATIEMIAEHPGMGQPDENLRAGYRRCASGSHVVFYRVGVRVE
IIRVLHQSMNARAHLG
Download Length: 291 bp
>T228 NC_002696:c968968-968678 [Caulobacter vibrioides CB15]
ATGAAGCCCTATCGGCTATCCCGTCGCGCCAAGGCCGACCTCGATGACATCTGGACCTATTCAGAGCAGCGATGGGGCGT
TGAACAAGCCGCCGACTACGCCCGTGAACTGCAGGCCACCATCGAGATGATCGCTGAACATCCGGGTATGGGACAGCCGG
ATGAAAACCTCCGCGCGGGCTACAGGCGATGCGCCAGCGGCTCGCACGTGGTCTTTTACCGCGTCGGCGTGCGGGTCGAG
ATTATCCGCGTGCTACACCAAAGCATGAACGCCAGGGCTCACCTTGGCTGA
ATGAAGCCCTATCGGCTATCCCGTCGCGCCAAGGCCGACCTCGATGACATCTGGACCTATTCAGAGCAGCGATGGGGCGT
TGAACAAGCCGCCGACTACGCCCGTGAACTGCAGGCCACCATCGAGATGATCGCTGAACATCCGGGTATGGGACAGCCGG
ATGAAAACCTCCGCGCGGGCTACAGGCGATGCGCCAGCGGCTCGCACGTGGTCTTTTACCGCGTCGGCGTGCGGGTCGAG
ATTATCCGCGTGCTACACCAAAGCATGAACGCCAGGGCTCACCTTGGCTGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9635.57 Da Isoelectric Point: 4.1523
>AT228 WP_010918759.1 NC_002696:c969231-968965 [Caulobacter vibrioides CB15]
MASKNTSVVLGDHFQAFIDSQVADGRYGSASEVIRAGLRLLEENEAKLAALRAALIEGEESGFIEDFDFDAFIEERSRAS
APQGFHEE
MASKNTSVVLGDHFQAFIDSQVADGRYGSASEVIRAGLRLLEENEAKLAALRAALIEGEESGFIEDFDFDAFIEERSRAS
APQGFHEE
Download Length: 267 bp
>AT228 NC_002696:c969231-968965 [Caulobacter vibrioides CB15]
ATGGCGAGCAAGAACACCTCCGTCGTCCTGGGCGATCACTTCCAGGCGTTCATCGACAGCCAGGTCGCTGACGGGCGCTA
CGGCTCGGCCAGCGAAGTCATCCGCGCGGGGCTGAGGCTCCTCGAAGAGAACGAAGCGAAGCTCGCGGCCCTCCGCGCCG
CCCTGATCGAAGGCGAGGAGAGCGGCTTTATCGAGGACTTCGACTTCGACGCCTTTATCGAGGAAAGGTCTAGGGCATCC
GCTCCGCAGGGGTTCCATGAGGAATGA
ATGGCGAGCAAGAACACCTCCGTCGTCCTGGGCGATCACTTCCAGGCGTTCATCGACAGCCAGGTCGCTGACGGGCGCTA
CGGCTCGGCCAGCGAAGTCATCCGCGCGGGGCTGAGGCTCCTCGAAGAGAACGAAGCGAAGCTCGCGGCCCTCCGCGCCG
CCCTGATCGAAGGCGAGGAGAGCGGCTTTATCGAGGACTTCGACTTCGACGCCTTTATCGAGGAAAGGTCTAGGGCATCC
GCTCCGCAGGGGTTCCATGAGGAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T105 | Mycobacterium tuberculosis H37Rv |
43.617 |
97.917 |
0.427 |
| T169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
38.776 |
100 |
0.396 |
| T10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
35.165 |
94.792 |
0.333 |
| T233 | Caulobacter crescentus CB15 |
38.095 |
87.5 |
0.333 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT105 | Mycobacterium tuberculosis H37Rv |
59.259 |
97.59 |
0.578 |
| AT169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
63.014 |
91.25 |
0.575 |
| AT10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
45.349 |
100 |
0.453 |
| AT233 | Caulobacter crescentus CB15 |
35.443 |
95.181 |
0.337 |
Multiple sequence alignment
References
(1) Kevin M Dalton et al. (2010) A conserved mode of protein recognition and binding in a ParD-ParE toxin-antitoxin complex. Biochemistry 49(10):2205-15. [PubMed:20143871]