Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/RHH-RelE |
| Location | 2203681..2204225 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | P9WHG7 |
| Locus tag | Rv1959c | Protein ID | NP_216475.1 |
| Coordinates | 2203681..2203977 (-) | Length | 99 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | P9WIJ7 |
| Locus tag | Rv1960c | Protein ID | NP_216476.1 |
| Coordinates | 2203974..2204225 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1948c | 2198714..2199064 | - | 351 | NP_216464.1 | hypothetical protein | - |
| Rv1950c | 2199998..2200189 | - | 192 | NP_216466.1 | hypothetical protein | - |
| Rv1951c | 2200190..2200486 | - | 297 | NP_216467.1 | hypothetical protein | - |
| Rv1952 | 2200726..2200941 | + | 216 | NP_216468.1 | antitoxin VapB14 | - |
| Rv1953 | 2200938..2201249 | + | 312 | NP_216469.1 | ribonuclease VapC14 | - |
| Rv1954c | 2201223..2201744 | - | 522 | NP_216470.1 | hypothetical protein | - |
| Rv1955 | 2201719..2202096 | + | 378 | NP_216471.2 | toxin HigB | - |
| Rv1956 | 2202138..2202587 | + | 450 | NP_216472.1 | antitoxin HigA | - |
| Rv1957 | 2202584..2203129 | + | 546 | NP_216473.1 | SecB-like chaperone | - |
| Rv1958c | 2203018..2203632 | - | 615 | NP_216474.1 | hypothetical protein | - |
| Rv1959c | 2203681..2203977 | - | 297 | NP_216475.1 | toxin ParE1 | Toxin |
| Rv1960c | 2203974..2204225 | - | 252 | NP_216476.1 | antitoxin ParD1 | Antitoxin |
| Rv1961 | 2204212..2204706 | + | 495 | NP_216477.1 | hypothetical protein | - |
| Rv1962c | 2204866..2205273 | - | 408 | NP_216478.1 | ribonuclease VapC35 | - |
| Rv1962A | 2205277..2205549 | - | 273 | YP_007410641.1 | antitoxin VapB35 | - |
| Rv1963c | 2205582..2206802 | - | 1221 | NP_216479.1 | transcriptional repressor Mce3R | - |
| Rv1964 | 2207700..2208497 | + | 798 | NP_216480.1 | integral membrane protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 99 a.a. Molecular weight: 11268.64 Da Isoelectric Point: 6.8604
>T105 NP_216475.1 NC_000962:c2203977-2203681 [Mycobacterium tuberculosis H37Rv]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL
Download Length: 297 bp
>T105 NC_000962:c2203977-2203681 [Mycobacterium tuberculosis H37Rv]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9207.17 Da Isoelectric Point: 7.0150
>AT105 NP_216476.1 NC_000962:c2204225-2203974 [Mycobacterium tuberculosis H37Rv]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR
Download Length: 252 bp
>AT105 NC_000962:c2204225-2203974 [Mycobacterium tuberculosis H37Rv]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
46.154 |
92.857 |
0.429 |
| T228 | Caulobacter crescentus CB15 |
43.617 |
97.917 |
0.427 |
| T10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
36.735 |
100 |
0.367 |
| T233 | Caulobacter crescentus CB15 |
37.079 |
90.816 |
0.337 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT228 | Caulobacter crescentus CB15 |
59.259 |
97.59 |
0.578 |
| AT169 | Vibrio cholerae O1 biovar El Tor str. N16961 |
54.795 |
91.25 |
0.5 |
| AT10079 | Pseudoalteromonas rubra strain SCSIO 6842 |
43.243 |
89.157 |
0.386 |
Multiple sequence alignment
References
(1) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]